Thermostable alpha-amylase production using Bacillus licheniformis NRRL B14368.

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dc.contributor.authorBose, Ken_US
dc.contributor.authorDas, Den_US
dc.date.accessioned2009-05-28T12:34:28Z
dc.date.available2009-05-28T12:34:28Z
dc.date.issued1996-12-01en_US
dc.description.abstractMaximum amount of extracellular alpha-amylase of B. licheniformis NRRL B14368 was obtained at the stationary phase. Highest yield of alpha-amylase was achieved with high level of crude protein and low carbohydrate level. There was a catabolite repression in the organism. Protease was produced concurrently with alpha-amylase. It was also observed that soyabean acts as an inhibitor of the protease. Optimum pH and temperature of alpha-amylase were 5-7 and 76 degrees C respectively. It was also observed that alpha-amylase production was a non-growth associated product. Maltose was an excellent inducer for alpha-amylase production. Ca2+ (0.01 M) increased the thermostability of the enzyme. Alpha-amylase purification studies were carried out by using isopropanol, acetone, ammonium sulphate solution, ion exchange chromatography. Acetone was found most suitable for the separation of alpha-amylase. Protein recovery and relative enzyme activity (as compared to that of the maximum activity of the crude enzyme) were 30.77% and 3.03 respectively.en_US
dc.description.affiliationBiotechnology Centre, Indian Institute of Technology, Kharagpur, India.en_US
dc.identifier.citationBose K, Das D. Thermostable alpha-amylase production using Bacillus licheniformis NRRL B14368. Indian Journal of Experimental Biology. 1996 Dec; 34(12): 1279-82en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/58266
dc.language.isoengen_US
dc.source.urihttps://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijeb/ijeb0.aspen_US
dc.subject.meshBacillus --enzymologyen_US
dc.subject.meshEnzyme Stabilityen_US
dc.subject.meshFermentationen_US
dc.subject.meshHydrogen-Ion Concentrationen_US
dc.subject.meshTemperatureen_US
dc.subject.meshalpha-Amylases --biosynthesisen_US
dc.titleThermostable alpha-amylase production using Bacillus licheniformis NRRL B14368.en_US
dc.typeJournal Articleen_US
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