Sex determination by amplification of amelogenin gene from dental pulp tissue by polymerase Chain Reaction

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dc.contributor.authorChowdhury, Ripon Mden_US
dc.contributor.authorSinghvi, Abhisheken_US
dc.contributor.authorBagul, Neetaen_US
dc.contributor.authorBhatia, Sanyaen_US
dc.contributor.authorSingh, Gurdeepen_US
dc.contributor.authorGoswami, Surajiten_US
dc.date.accessioned2020-01-02T06:45:23Z
dc.date.available2020-01-02T06:45:23Z
dc.date.issued2018-07
dc.description.abstractForensic odontology necessarily involves the application of dentistry along with various other branches of sciences which deals with proper handling, examination, evaluation, and presentation of dental evidences, that aids to investigate a crime and deliver justice. Sex determination is a part of forensic odontology and an essential priority when traditional identification of the deceased becomes impossible. Aim: To determine Sex by analysis of the Amelogenin gene using Polymerase Chain Reaction (PCR) method on Deoxyribose nucleic acid (DNA) isolated from dental pulp, which was exposed to various environmental conditions created artificially to mimic a forensic scenario. Materials and Method: This in-vitro study was conducted by subjecting extracted teeth to various conditions imitating a forensic scene, viz. desiccation at room temperatures, immersion in salt water, burial in soil and even exposing to extremes of temperatures. DNA was extracted from dental pulp tissue and sex determination was achieved by amplification of the amelogenin gene through AMEL gene based primers in PCR. Result: Among all the samples used in this study, DNA could be extracted from all, except from those that were subjected to a temperature of 350 °C. DNA amplification and sex determination of the samples were found to be accurate when compared to sex of the individual which was recorded initially, during collection of teeth samples. Conclusion: This study shows teeth to be a potent source of DNA even in extreme environmental conditions, barring high temperatures and determination of sex by PCR amplification of AMEL markers to be quite reliable.en_US
dc.identifier.affiliationsDepartment of Oral and Maxillofacial Pathology, Mithila Minority Dental College and Hospital, Laheriasarai, Darbhanga, Bihar, Indiaen_US
dc.identifier.affiliationsDepartment of Oral and Maxillofacial Pathology, Vyas Dental College, Jodhpur, Rajasthan, Indiaen_US
dc.identifier.affiliationsMedinova, West Bengal, Indiaen_US
dc.identifier.citationChowdhury Ripon Md, Singhvi Abhishek, Bagul Neeta, Bhatia Sanya, Singh Gurdeep, Goswami Surajit. Sex determination by amplification of amelogenin gene from dental pulp tissue by polymerase Chain Reaction. Indian Journal of Dental Research. 2018 Jul; 29(4): 470-476en_US
dc.identifier.issn0970-9290
dc.identifier.issn1998-3603
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/192100
dc.languageenen_US
dc.publisherIndian Society for Dental Researchen_US
dc.relation.issuenumber4en_US
dc.relation.volume29en_US
dc.source.urihttps://dx.doi.org/10.4103/ijdr.IJDR_274_17en_US
dc.subjectAmelogeninen_US
dc.subjectdental pulpen_US
dc.subjectDNAen_US
dc.subjectenvironmental conditionsen_US
dc.subjectsex determinationen_US
dc.titleSex determination by amplification of amelogenin gene from dental pulp tissue by polymerase Chain Reactionen_US
dc.typeJournal Articleen_US
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