1-Naphthyl acetate as an alternative substrate of hemolysate cholinesterase: Direct visualization of enzyme activity within 10 minutes on polyacrylamide gels

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dc.contributor.authorChowdhary, Sheemonaen_US
dc.contributor.authorBhattacharyya, Rajasrien_US
dc.contributor.authorBanerjee, Dibyajyotien_US
dc.date.accessioned2020-01-02T06:38:43Z
dc.date.available2020-01-02T06:38:43Z
dc.date.issued2019-09
dc.description.abstractHemolysate cholinesterase is currently recognized as the most preferred biomarker to detect acute organophosphorus poisoning. Direct visualization of cholinesterase activity on polyacrylamide gels is routinely practiced using acetylthiocholine as a substrate. Overnight incubation with the staining solution is required to understand the enzyme activity bands on gels. Therefore, the need arises to explore rapid detection methods, which can specifically detect hemolysate cholinesterase on polyacrylamide gels. Here, we have explored alternative substrates, such as 1-NA and 2-NA which might have the potential to behave as specific substrates for the detection of hemolysate cholinesterase activity on the gels. It is observed by the in silico studies that 1-NA bind at the active site of acetylcholinesterase akin to acetylcholine (ACh) with a better fitness score. Secondly, the hemolysate cholinesterase activity, as well as its inhibition by organophosphorus pesticides is understandable within 10 min using Fast Blue RR dye for the detection of 1-NA. The organophosphorus inhibited activity is regained in the presence of cholinesterase reactivator. Moreover, the enzyme activity bands formed using 1-NA proves the specificity of the substrate for hemolysate cholinesterase as in the presence of specific acetylcholinesterase inhibitors the band formation disappears. On the other hand, ATCh requires minimum 8-12 h staining time for detection of enzyme activity band following Karnovsky and Roots protocol. Our results prove that 1-NA is an alternative substrate of hemolysate cholinesterase which specifically detects the enzyme activity on gel rapidly. We recommend 1-NA for rapid detection of hemolysate cholinesterase activity on the gels.en_US
dc.identifier.affiliationsDepartment of Experimental Medicine and Biotechnology, Postgraduate Institute of Medical Education and Research, Chandigarh-160 012, Indiaen_US
dc.identifier.citationChowdhary Sheemona, Bhattacharyya Rajasri, Banerjee Dibyajyoti. Stress induced changes in osmoprotectants, ionic relations, antioxidants activities and protein profiling characterize Sporobolus marginatus Hochst. ex A. Rich. salt tolerance mechanism. Indian Journal of Experimental Biology. 2019 Sep; 57(9): 672-679en_US
dc.identifier.issn0975-1009
dc.identifier.issn0019-5189
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/191509
dc.languageenen_US
dc.publisherNISCAIR-CSIR, Indiaen_US
dc.relation.issuenumber9en_US
dc.relation.volume57en_US
dc.source.urihttps://nopr.niscair.res.in/handle/123456789/50453en_US
dc.title1-Naphthyl acetate as an alternative substrate of hemolysate cholinesterase: Direct visualization of enzyme activity within 10 minutes on polyacrylamide gelsen_US
dc.typeJournal Articleen_US
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