Evaluation of a colorimetric method for vitamin A estimation.
dc.contributor.author | Panth, M | en_US |
dc.contributor.author | John, A | en_US |
dc.contributor.author | Sivakumar, B | en_US |
dc.date.accessioned | 2009-05-28T16:36:33Z | |
dc.date.available | 2009-05-28T16:36:33Z | |
dc.date.issued | 1989-12-01 | en_US |
dc.description.abstract | A simple colorimetric procedure for plasma vitamin A is evaluated, which does not require sophisticated or expensive equipment. Vitamin A values obtained with 30 human plasma samples and 11 liver samples obtained from lactating rabbits, using a colorimetric procedure based on Carr-Price reaction with ferric chloride and acetyl chloride were compared with those obtained with spectrophotometric and spectrofluorimetric micro methods. With widely varying plasma samples, the values showed a high degree correlation and good agreement. The intraassay variation was 3% which is in the acceptable range. The plasma samples could be analysed within 4 weeks and the reagents, were found to be stable, unlike some batches of trifluoro acetic acid (TFA). | en_US |
dc.identifier.citation | Panth M, John A, Sivakumar B. Evaluation of a colorimetric method for vitamin A estimation. Indian Journal of Experimental Biology. 1989 Dec; 27(12): 1044-7 | en_US |
dc.identifier.uri | https://imsear.searo.who.int/handle/123456789/63447 | |
dc.language.iso | eng | en_US |
dc.source.uri | https://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijeb/ijeb0.asp | en_US |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Carotenoids --analysis | en_US |
dc.subject.mesh | Colorimetry --methods | en_US |
dc.subject.mesh | Female | en_US |
dc.subject.mesh | Humans | en_US |
dc.subject.mesh | Male | en_US |
dc.subject.mesh | Pregnancy | en_US |
dc.subject.mesh | Rabbits | en_US |
dc.subject.mesh | Spectrometry, Fluorescence | en_US |
dc.subject.mesh | Spectrophotometry | en_US |
dc.subject.mesh | Vitamin A --analysis | en_US |
dc.title | Evaluation of a colorimetric method for vitamin A estimation. | en_US |
dc.type | Comparative Study | en_US |
dc.type | Journal Article | en_US |
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