Improvement of Bacillus strains by mutation for overproduction of exopolygalacturonases.

Simple item page
dc.contributor.authorMuzzamal, Hira
dc.contributor.authorLatif, Zakia
dc.date.accessioned2016-11-24T06:55:18Z
dc.date.available2016-11-24T06:55:18Z
dc.date.issued2016-08
dc.description.abstractPectinases, produced by microorganisms, have wide range application in food industry, textile processing, paper making, coffee and tea fermentation, etc. It accounts for 10% of the global industrial enzymes produced. The most important and widely used commercial pectinase polygalacturonase is produced by alkalophilic strains of Bacillus sp. and Streptomyces sp. Here, we explored 29 bacterial strains isolated from rotten mango samples for polygalacturonase production and selected 16 strains through preliminary screening by well-plate method for enzyme activity. The maximum zone of inhibition of pectin was observed up to 28 mm in diameter but one strain ZM11 was exhibiting no activity. Quantitative dinitrisalicylic acid (DNS) assay for polygalacturonase enzyme was also performed for the selected bacterial isolates. All the strains bestowed significant enzyme activity with the highest activity of 2.4 U/µL exhibited by strain ZM3 (P ≤0.05). Characterization of the isolates was performed using different biochemical tests which also confirmed the isolates as members of the genus Bacillus. Mutation was induced to the selected strains by UV light and acridine orange for different periods of time. Qualitative and quantitative assays of the mutant bacterial isolates showed that the enzyme activity increased to 4.62 U/µL which clearly indicated that induced mutation enhanced the ability of Bacillus strains to produce more polygalacturonase enzyme up to 3-fold as compared to the wild strains (P ≤0.05). Molecular characterization by 16S rRNA sequences further confirmed that the bacterial isolates belong to Bacillus subtilis and B. amyloliquefaciens.en_US
dc.identifier.citationMuzzamal, Hira Latif, Zakia. Improvement of Bacillus strains by mutation for overproduction of exopolygalacturonases. Indian Journal of Experimental Biology. 2016 Aug; 54(8): 509.517.en_US
dc.identifier.issn0975-1009
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/178801
dc.language.isoenen_US
dc.source.urihttps://nopr.niscair.res.in/handle/123456789/35142en_US
dc.subject16S rRNA sequencingen_US
dc.subjectAcridine orangeen_US
dc.subjectDinitrisalicylic aciden_US
dc.subjectPectinasesen_US
dc.subjectPolygalacturonase (PG) activityen_US
dc.subjectUV mutagenesisen_US
dc.titleImprovement of Bacillus strains by mutation for overproduction of exopolygalacturonases.en_US
dc.typeArticleen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
ijeb2016v54n8p509.pdf
Size:
310.72 KB
Format:
Adobe Portable Document Format
Description:
Journal article
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Indian Journal of Experimental Biology