Preparation of Protein Extraction from Flower Buds of Solanum lycopersicum for Two- Dimensional Gel Electrophoresis.

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dc.contributor.authorZhao, Xiaocui
dc.contributor.authorRen, Jingqi
dc.contributor.authorCui, Na
dc.contributor.authorFan, Haiyan
dc.contributor.authorYu, Guangchao
dc.contributor.authorLi, Tianlai
dc.date.accessioned2015-08-25T05:39:57Z
dc.date.available2015-08-25T05:39:57Z
dc.date.issued2013-04
dc.description.abstractAims: Find a suitable method for the protein extraction from flower buds of Solanum lycopersicum. Study Design: Compare some kinds of protein extraction methods and find the best one among them suitable to tomato flower buds. Place and Duration of Study: Biological Science and Technology College, between June 2010 and July 2011. Methodology: The proteins for electrophoresis were extracted using different methods, such as trichloroacetic acid /acetone (TCA/acetone), Sodium dodecyl sulfate (SDS), Trissaturated phenol (Tris-Phen), Phenol/SDS and Direct lysis method. After silver staining, different patterns of protein spots were observed in the gels. Results: Few spots were found by SDS and Phenol/SDS extractions, more spots by immediate dissolution but the most impurities, less protein productivity though more spots by Tris-Phen extractions, and more protein productivity and better apart effect by TCA/acetone. The 2-DE image background was the clear and the protein spots were the most by TCA/acetone method. Conclusion: TCA/acetone method is much more suitable as extraction method for protein two-dimensional electrophoresis of tomato flower buds.en_US
dc.identifier.citationZhao Xiaocui, Ren Jingqi, Cui Na, Fan Haiyan, Yu Guangchao, Li Tianlai. Preparation of Protein Extraction from Flower Buds of Solanum lycopersicum for Two- Dimensional Gel Electrophoresis. British Biotechnology Journal. 2013 Apr; 3(2): 183-190.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/162414
dc.language.isoenen_US
dc.source.urihttps://sciencedomain.org/abstract/1155en_US
dc.subjectFlower budsen_US
dc.subjectprotein extractionen_US
dc.subjectSolanum lycopersicumen_US
dc.subjecttwo-dimensional gel electrophoresisen_US
dc.titlePreparation of Protein Extraction from Flower Buds of Solanum lycopersicum for Two- Dimensional Gel Electrophoresis.en_US
dc.typeArticleen_US
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