Evaluation of antimitotic activity of Rotula aquatica (Lour): a traditional herb used in treatment of cancer.

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dc.contributor.authorPatil, Sen_US
dc.contributor.authorNarayanan, Sen_US
dc.contributor.authorEibl, Gen_US
dc.contributor.authorJolly, C Ien_US
dc.date.accessioned2009-05-28T12:27:30Z
dc.date.available2009-05-28T12:27:30Z
dc.date.issued2004-09-07en_US
dc.description.abstractRotula aquatica was extensively used by vaidyas (Ayurvedic practioners) in holistic treatment of cancer. In the present study, an attempt has been made to evaluate the antimitotic activity of R. aquatica. Preliminary antimitotic screening was done using Allium cepa root tip assay. The mitotic index of the root tips markedly decreased with increasing concentration of the aqueous extract. The different fractions obtained by successive extraction of R. aquatica using solvents of increasing polarity were also evaluated for their antimitotic activity. Tannins were isolated which showed a better activity than the non-tannin fraction. Experiments were also carried out with incorporation of folic acid in the aqueous extract. Folic acid inhibited the antimitotic activity of aqueous extract of R. aquatica in a dose dependent manner. The results obtained were compared with methotrexate--a known drug available in market as anti-cancer agent. The studies were extended to human cells using 3 pancreatic cancer cell lines, viz: HPAF-II, BxPC-3, and CAPAN-2. Extract of R. aquatica was found to be extremely effective in the prevention of cell proliferation of the pancreatic cancer cell lines. The phytochemical evaluation revealed presence of polyphenols (tannins) and steroids. A HPTLC fingerprinting was developed and studied. Two compounds were isolated and subjected to spectral studies like UV, IR and mass spectrums. The empirical formula was derived by considering this data with elemental analysis of the compounds.en_US
dc.description.affiliationDepartment of Pharmacognosy and Phytochemistry, Prin K M Kundnani College of Pharmacy, 47, R G Thadani Marg, Worli, Mumbai 400 018, India. s_patil@vsnl.comen_US
dc.identifier.citationPatil S, Narayanan S, Eibl G, Jolly CI. Evaluation of antimitotic activity of Rotula aquatica (Lour): a traditional herb used in treatment of cancer. Indian Journal of Experimental Biology. 2004 Sep; 42(9): 893-9en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/58109
dc.language.isoengen_US
dc.source.urihttps://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijeb/ijeb0.aspen_US
dc.subject.meshAllium --chemistryen_US
dc.subject.meshAntimetabolites, Antineoplastic --pharmacologyen_US
dc.subject.meshBoraginaceae --chemistryen_US
dc.subject.meshCell Division --drug effectsen_US
dc.subject.meshChromatography, High Pressure Liquiden_US
dc.subject.meshFolic Acid --pharmacologyen_US
dc.subject.meshMass Spectrometryen_US
dc.subject.meshMethotrexate --pharmacologyen_US
dc.subject.meshMitotic Indexen_US
dc.subject.meshPancreatic Neoplasms --drug therapyen_US
dc.subject.meshPhytotherapyen_US
dc.subject.meshPlant Extracts --therapeutic useen_US
dc.subject.meshPlant Roots --cytologyen_US
dc.subject.meshSteroids --chemistryen_US
dc.subject.meshTannins --metabolismen_US
dc.titleEvaluation of antimitotic activity of Rotula aquatica (Lour): a traditional herb used in treatment of cancer.en_US
dc.typeComparative Studyen_US
dc.typeJournal Articleen_US
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