Anti-bacterial activity of Achatina CRP and its mechanism of action.

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dc.contributor.authorMukherjee, Sandip
dc.contributor.authorBarman, Soma
dc.contributor.authorSarkar, Shuvasree
dc.contributor.authorMandal, Narayan Chandra
dc.contributor.authorBhattacharya, Shelley
dc.date.accessioned2014-12-11T08:25:42Z
dc.date.available2014-12-11T08:25:42Z
dc.date.issued2014-07
dc.description.abstractThe physiological role of C-reactive protein (CRP), the classical acute-phase protein, is not well documented, despite many reports on biological effects of CRP in vitro and in model systems in vivo. It has been suggested that CRP protects mice against lethal toxicity of bacterial infections by implementing immunological responses. In Achatina fulica CRP is a constitutive multifunctional protein in haemolymph and considered responsible for their survival in the environment for millions of years. The efficacy of Achatina CRP (ACRP) was tested against both Salmonella typhimurium and Bacillus subtilis infections in mice where endogenous CRP level is negligible even after inflammatory stimulus. Further, growth curves of the bacteria revealed that ACRP (50 µg/mL) is bacteriostatic against gram negative salmonellae and bactericidal against gram positive bacilli. ACRP induced energy crises in bacterial cells, inhibited key carbohydrate metabolic enzymes such as phosphofructokinase in glycolysis, isocitrate dehydrogenase in TCA cycle, isocitrate lyase in glyoxylate cycle and fructose-1,6-bisphosphatase in gluconeogenesis. ACRP disturbed the homeostasis of cellular redox potential as well as reduced glutathione status, which is accompanied by an enhanced rate of lipid peroxidation. Annexin V-Cy3/CFDA dual staining clearly showed ACRP induced apoptosis-like death in bacterial cell population. Moreover, immunoblot analyses also indicated apoptosis-like death in ACRP treated bacterial cells, where activation of poly (ADP-ribose) polymerase-1 (PARP) and caspase-3 was noteworthy. It is concluded that metabolic impairment by ACRP in bacterial cells is primarily due to generation of reactive oxygen species and ACRP induced anti-bacterial effect is mediated by metabolic impairment leading to apoptosis-like death in bacterial cells.en_US
dc.identifier.citationMukherjee Sandip, Barman Soma, Sarkar Shuvasree, Mandal Narayan Chandra, Bhattacharya Shelley. Anti-bacterial activity of Achatina CRP and its mechanism of action. Indian Journal of Experimental Biology. 2014 Jul; 52(7): 692-704.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/153749
dc.language.isoenen_US
dc.source.urihttps://nopr.niscair.res.in/handle/123456789/29042en_US
dc.subjectAchatina fulicaen_US
dc.subjectApoptosis-like-deathen_US
dc.subjectC-reactive proteinen_US
dc.subjectMetabolic enzymesen_US
dc.subjectOxidative stressen_US
dc.subject.meshAnimals
dc.subject.meshAnti-Bacterial Agents --pharmacology
dc.subject.meshApoptosis --drug effects
dc.subject.meshBacillus subtilis --drug effects
dc.subject.meshBacillus subtilis --metabolism
dc.subject.meshC-Reactive Protein --isolation & purification
dc.subject.meshC-Reactive Protein --pharmacology
dc.subject.meshGluconeogenesis --drug effects
dc.subject.meshGlycolysis --drug effects
dc.subject.meshGram-Negative Bacterial Infections --drug therapy
dc.subject.meshGram-Negative Bacterial Infections --metabolism
dc.subject.meshGram-Negative Bacterial Infections --microbiology
dc.subject.meshHemolymph --metabolism
dc.subject.meshHomeostasis --drug effects
dc.subject.meshImmunoblotting
dc.subject.meshLipid Peroxidation --drug effects
dc.subject.meshMale
dc.subject.meshMice
dc.subject.meshOxidation-Reduction
dc.subject.meshReactive Oxygen Species --metabolism
dc.subject.meshSalmonella Infections --drug therapy
dc.subject.meshSalmonella Infections --metabolism
dc.subject.meshSalmonella Infections --microbiology
dc.subject.meshSalmonella typhimurium --drug effects
dc.subject.meshSalmonella typhimurium --metabolism
dc.subject.meshSnails
dc.titleAnti-bacterial activity of Achatina CRP and its mechanism of action.en_US
dc.typeArticleen_US
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