In vitro responsiveness of hamster corpora lutea undergoing luteolysis to luteinizing hormone.
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Date
1979-12
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Abstract
Corpora lutea removed from pregnant hamster deprived of endogenous
luteinizing hormone for varying periods were compared for their responsiveness to
externally added luteinizing hormone. The corpora lutea removed on the 8th
day of pregnancy exhibited a dose-dependent increase in progesterone production
in response to added luteinizing hormone upto a concentration of 2·5 μg/ml. The
total progesterone synthesised by the corpora lutea decreased with increase in the
duration of in vivo luteinizing hormone deprivation. However, the hormone deprivation
had to be for a minimum period of 24 h before a marked reduction in the
in vitro responsiveness could be seen. Neutralisation of endogenous luteinizing
hormone increased the luteal cholesterol ester concentration, while in vitro incubation
of such tissue with luteinizing hormone resulted in a marked reduction in cholesterol
ester levels. Corpora lutea removed from hamsters on day 8, 15 and 16 of pregnancy
when compared for their responsiveness in vitro to added luteinizing hormone
showed that the luteal tissue of day 8 produced more progesterone relative to those
of day 15/16. In contrast, depletion of free and esterified cholesterol increased
with the increase in age of corpora lutea (from 15% on day 8 to 67% on day 16).
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Keywords
Luteolysis, luteinizing hormone, progesterone, hamster
Citation
Mukku Venkat Ramaiah, Moudgal N R. In vitro responsiveness of hamster corpora lutea undergoing luteolysis to luteinizing hormone. Journal of Biosciences. 1979 Dec; 1(4): 457-465.