Predictive WBC count and differential WBC on time variation.
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Date
2008-09
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Abstract
Objective: To evaluate the storage time that the concentration of all blood parameters are stable and to propose the predictive formula for the unstable blood parameters.
Methods: EDTA blood samples from 22 healthy volunteer subjects were aliquot and separated into 2 parts, one kept at refrigerator temperature (4oC) and the other kept at room temperature (RT). Complete blood counts (CBC) were determined in each blood sample with a CELL DYN 3200 hematology analyzer every hour for 24 hours and every 2 hours from 26 to 72 hours.
Results: The RBC count, RBC indicies, platelet count, and differential WBC were stable for 72 hr blood storage at 4oC except the WBC count was decreased after 15 hr storage. The predictive WBC count after 15 hr storage at 4oC was WBC0 = WBCT + 0.081(T-15).
Conclusion: This study establishes the predictive formula for the initial time value which is useful for laboratories that have a problem with instrument failure or for mobile units that cannot measure CBC immediately.
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Keywords
Complete blood count, CELL DYN 3200, predictive value, stability
Citation
Tangvarasittichai Orathai, Tangvarasittichai Surapon. Predictive WBC count and differential WBC on time variation. Siriraj Medical Journal, 2008 Sept; 60(5): 248-250.