Protein mediated cholesterol absorption in locusts Schistocerca gregaria (Forskal) and Locusta migratoria (Linn).

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dc.contributor.authorUpadhyay, Ravi Kanten_US
dc.contributor.authorAgarwal, H Cen_US
dc.contributor.authorDhar, Ravien_US
dc.date.accessioned2009-05-28T10:41:41Z
dc.date.available2009-05-28T10:41:41Z
dc.date.issued2002-02-08en_US
dc.description.abstractAbsorption and transport of 3H cholesterol from the midgut to hemolymph and other tissues was studied in the locusts Schistocerca gregaria and Locusta migratoria. S. gregaria are able to absorb dietary cholesterol in the midgut and release into the hemolymph in vivo and into the incubation medium in virto. Certain proteins of midgut origin are involved in the absorption and release of cholesterol. The proteins designated as cholesterol binding proteins (CBP's) were fractionated by gel filtration chromatography using Sepharose CL-6B-200 column. Presence of a protein and its binding with cholesterol is confirmed by TCA precipitation after subsequent incubation of midgut in the incubation medium. Cholesterol binding with the proteins was also confirmed in native polyacrylamide gel electrophoresis. Biosynthesis of this protein takes place in the midgut which is inhibited by a protein synthesis inhibitor, cycloheximide. It also inhibits absorption and release of cholesterol from the midgut. The cholesterol binding activity was associated with a peak containing proteins ranging from molecular weights of 17-32 kDa in SDS-PAGE gels. Treatment of midgut with cycloheximide resulted in reduced cholesterol binding activity. Dilipidation of mucin and transport in presence of bile salts yielded a higher cholesterol binding activity. Although the absorption and release of cholesterol was observed in the hemolymph of both sexes, the ovary exhibited higher cholesterol binding as compared to testis.en_US
dc.description.affiliationDepartment of Zoology, University of Delhi, Delhi 110 007, India. rkupadhya@yahoo.comen_US
dc.identifier.citationUpadhyay RK, Agarwal HC, Dhar R. Protein mediated cholesterol absorption in locusts Schistocerca gregaria (Forskal) and Locusta migratoria (Linn). Indian Journal of Experimental Biology. 2002 Feb; 40(2): 151-61en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/55665
dc.language.isoengen_US
dc.source.urihttps://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijeb/ijeb0.aspen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCholesterol --metabolismen_US
dc.subject.meshChromatography --methodsen_US
dc.subject.meshCycloheximide --pharmacologyen_US
dc.subject.meshElectrophoresis, Polyacrylamide Gelen_US
dc.subject.meshFemaleen_US
dc.subject.meshGrasshoppersen_US
dc.subject.meshHemolymph --metabolismen_US
dc.subject.meshMaleen_US
dc.subject.meshOvary --metabolismen_US
dc.subject.meshProtein Bindingen_US
dc.subject.meshProtein Synthesis Inhibitors --pharmacologyen_US
dc.subject.meshSepharose --pharmacologyen_US
dc.subject.meshTestis --metabolismen_US
dc.subject.meshTime Factorsen_US
dc.subject.meshUltracentrifugationen_US
dc.titleProtein mediated cholesterol absorption in locusts Schistocerca gregaria (Forskal) and Locusta migratoria (Linn).en_US
dc.typeJournal Articleen_US
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