Cloning and expression of a small heat and salt tolerant protein (Hsp22) from Chaetomium globosum.

Simple item page
dc.contributor.authorAggarwal, Rashmi
dc.contributor.authorGupta, Sangeeta
dc.contributor.authorSharma, Sapna
dc.contributor.authorBanerjee, Sagar
dc.contributor.authorSingh, Priyanka
dc.date.accessioned2013-02-28T04:38:58Z
dc.date.available2013-02-28T04:38:58Z
dc.date.issued2012-11
dc.description.abstractThe present study reports molecular characterization of small heat shock protein gene in Indian isolates of Chaetomium globosum, C. perlucidum, C. reflexum, C. cochlioides and C. cupreum. Six isolates of C. globosum and other species showed a band of 630bp using specific primers. Amplified cDNA product of C. globosum (Cg 1) cloned and sequenced showed 603bp open reading frame encoding 200 amino-acids. The protein sequence had a molecular mass of 22 kDa and was therefore, named Hsp22. BlastX analysis revealed that the gene codes for a protein homologous to previously characterized Hsp22.4 gene from C. globosum (AAR36902.1, XP 001229241.1) and shared 95% identity in amino acid sequence. It also showed varying degree of similarities with small Hsp protein from Neurospora spp. (60%), Myceliophthora sp. (59%), Glomerella sp. (50%), Hypocrea sp. (52%), and Fusarium spp. (51%). This gene was further cloned into pET28a (+) and transformed E. coli BL21 cells were induced by IPTG, and the expressed protein of 30 kDa was analyzed by SDS-PAGE. The IPTG induced transformants displayed significantly greater resistance to NaCl and Na2CO3 stresses.en_US
dc.identifier.citationAggarwal Rashmi, Gupta Sangeeta, Sharma Sapna, Banerjee Sagar, Singh Priyanka. Cloning and expression of a small heat and salt tolerant protein (Hsp22) from Chaetomium globosum. Indian Journal of Experimental Biology. 2012 Nov; 50(11): 826-832.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/145322
dc.language.isoenen_US
dc.source.urihttps://nopr.niscair.res.in/handle/123456789/14944en_US
dc.subjectChaetomium globosumen_US
dc.subjectGene cloningen_US
dc.subjectSmall heat shock proteinen_US
dc.subjectStress toleranceen_US
dc.titleCloning and expression of a small heat and salt tolerant protein (Hsp22) from Chaetomium globosum.en_US
dc.typeArticleen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
ijeb2012v50n11p826.pdf
Size:
1.24 MB
Format:
Adobe Portable Document Format
Description:
Journal article
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Indian Journal of Experimental Biology