Update Ayurveda 2014 Abstracts Oral Presentation.
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2015-05
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Abstract
Oral delivery of Bromelain using calcium alginate beads
Devang Sarvaiya, Ujwala Shinde, Pankaj Jain,
Sadhana Sathaye1
1Department of Pharmaceutics, Bombay College of Pharmacy,
Santacruz, Kalina, Mumbai, 2Department of Pharmaceutical
Sciences and Technology, Institute of Chemical Technology,
Matunga, Mumbai, India
E-mail: ddsarvaiya@gmail.com
Background: The present research work was aimed at
preparing microspheres for oral delivery of bromelain, an
anti‑inflammatory enzyme from pineapple fruit (Ananas
comosus). Objective: The objective was to improve the
oral delivery of this proteolytic enzyme by circumventing
its instability in acidic environment and modulating its
release in intestine for its anti‑inflammatory action.
Materials and Methods: Sodium alginate, calcium
chloride, bromelain. Microspheres were prepared by
ionotropic gelatin technique dropping the sodium alginate
solution to magnetically stirred calcium chloride solution.
Results: Optimized batch of microspheres were prepared
using 3% sodium alginate concentration, 0.05M calcium
chloride and curing time of 20 min with highest entrapment
efficiency of 69.98%±0.087%. In vitro release studies
showed very low amount of drug release in 0.1N HCl and
sustained release in simulated intestinal fluid pH 6.8 with
more than 90% drug release at the end of 2 hrs. The drug
release profiles for the optimized batch was best fitted
with Korsmeyer‑Peppas as well as Higuchi model based on
regression coefficients. Bromelain microspheres showed
62.90%±1.44% edema inhibition in rat paw edema
model in comparison to marketed bromelain formulation
which showed 69.35% ±1.44% inhibition at end of 2
hrs. Optimized batch of microspheres were found to be
stable as per ICH guidelines of stability. Conclusion: Thus
alginate based microsphere drug delivery system can be
effectively developed for oral delivery of bromelain and can
be considered as an alternative approach to conventional
NSAIDs and side effects associated with it.
UA/OP/02
Analytical study to explore the pharmacokinetic aspect
of Basti (Rectal drug administration)
Rohini Purohit, B. S. Prasad, Sreelakshmi Chaganti,
Vedantam Giridhar
Department of Panchakarma, KLEU’S Shri BMK Ayurveda
Mahavidyalay, Belgaum, India
Email: dr.rohiniayu@gmail.com
Background: Niruha basti is one among the prime
Panchakarma procedures considered as ardha chikitsa and
also referred as purna chikitsa involves rectal route of drug
administration of the medicated formulation in a quantity
of 250‑300 ml (approx.). This treatment is widely being
practiced and has been proved efficacious in various disorders
like hemiplegia, sciatica, cardiac disorders, avascular
necrosis, diabetes, obesity, rheumatoid arthritis, etc., with
a retention period of 2 min‑10 min (approx.) on an average
as observed practically. Objective: A step forward now in
research towards understanding the pharmacokinetics of
basti which is frequently debated as a local action, placebo,
etc., was tried to explore with the help of technology
which is explained to have an action throughout the body.
Materials and Methods: Madhutailika basti was selected
as it has a single Kashaya dravya (medicine for decoction)
as Eranda (Ricinus communis Linn.) in large quantity along
with other drugs like honey, rock salt, moorchita tila
taila (processed sesamum oil) and Shatapushpa (Anethum
sowa Linn.) as kalka dravya. This basti was administered
to the male wistar rats. Results: The decoction of Eranda
and Madhutailika Basti which were subjected to UV analysis
showed similar peaks. The plasma samples of the rats
both with and without administration of basti showed a
significant variation along with the peaks similar to that
of the decoction and basti formulation on subjecting to
UV analysis. Conclusion: These evidences pave way
towards proving the systemic absorption of Madhutailika
basti in turn implying the mode of action of Niruha
basti (rectal drug administration).
UA/OP/03
Diosgenin ameliorates diabetes related renal
injury through suppression of oxidative stress and
inflammation
Divya M. Kanchan, Gauresh Somani, Vaibhavi Peshattiwar,
Sadhana Sathaye
Institute of Chemical Technology, Department of Pharmaceutical
Sciences & Technology, Matunga (E), Mumbai, India
Email: dmk86in@gmail.com
Background: Diabetes mellitus is a multi factorial metabolic
disorder associated with genesis of diabetes related vascular
diseases. Oxidative stress is claimed to be major impulsive
factor in the etiology of these diabetic complications.
Diosgenin, a steroidal saponin, reported to possess strong
anti-inflammatory and antioxidant activity which may prove
beneficial in combating oxidative stress and inflammatory
responses generated in diabetes. Objective: The current
study was undertaken to evaluate the effect of diosgenin
in early hyperglycemia induced diabetic complications in
kidney. Methods and Materials: Hyperglycemia was induced
by streptozotocin (STZ, 45 mg/kg, i.p.) in rats followed by
treatment with diosgenin (5, 10 & 20 mg/kg, oral 28 days).
Biochemical analysis of oxidative stress parameters such as
superoxide dismutase, catalase, reduced glutathione and lipid
peroxidation (LPO) were evaluated in kidney homogenates.
[Downloaded free from https://www.jaim.in on Monday, February 15, 2016, IP: 115.112.118.203]
Abstracts
These abstracts were presented at the Update Ayurveda 2014 at Mumbai, Maharashtra, India. The abstracts were screened
by the scientific committee of Update Ayurveda 2014 and are not peer reviewed by J-AIM. © Update Ayurveda 2014.
6
Biomarkers of kidney damage such as microalbuminuria,
kidney hypertrophy index and serum creatinine along with
histopathology of kidney was evaluated. Myeloperoxidase
levels were determined in kidney to evaluate the level
of inflammation. Results: The altered oxidative stress
levels in STZ induced diabetic rats were significantly
ameliorated as manifested by decreased LPO and increased
endogenous antioxidant levels by treatment of diosgenin in
a dose dependent manner. Blood glucose was significantly
decreased at 20 mg/kg. Microalbuminuria was significantly
prevented by diosgenin signifying its protective effect on
kidney which was confirmed by histopathological studies.
Decreased myeloperoxidase levels in diosgenin treatment
groups revealed its anti-inflammatory activity. Conclusion:
The study proves diosgenin as a promising candidate in
ameliorating diabetes associated complication through its
anti oxidant and anti-inflammatory activity.
UA/OP/04
Evaluation of anticonvulsant activity of Pistacia
integerrima in zebra fish and rodent models of epilepsy
Pankaj Jain, Rufi Tambe, Mruniya Nahire, Atul Bharadwaj,
Sadhana Sathaye
Department of Pharmaceutical Sciences and Technology,
Institute of Chemical Technology, Mumbai, India
E-mail: pdjain23@gmail.com
Background: Epilepsy is a one of the common CNS disorder
which is manifested by recurrent unprovoked epileptic
seizures. At neuronal level, seizure activity occurs when
glutamatergic excitatory neurotransmitters overrides
gamma‑aminobutyric acid (GABA) mediated inhibition.
Objective: The objective of the present study was to
evaluate Pistacia integerrima extracts for its anticonvulsant
activity in zebra fish and rodent model of epilepsy.
Materials and Methods: Petroleum ether (PEPI‑ 50, 100,
200 mg/kg) and methanolic (MEPI‑ 50 100, 200 mg/kg)
extracts were evaluated in Pentylenetetrazol (PTZ) induced
zebra fish model. The activity of the active extract was
further confirmed in PTZ induced convulsions in mice.
Further these extracts were screened in maximal shock
induced (MES) convulsions. Results: PEPI exhibited dose
dependent anticonvulsant activity in PTZ induced seizure in
zebra fish model which was further confirmed in PTZ and
MES rodent models. MEPI was ineffective in both the models
of epilepsy. Conclusion: PEPI extract demonstrated potential
anticonvulsant activity in zebra fish and rodent models. This
study provides the evidence that anticonvulsant activity of
PEPI extract could be due to modulation of GABAA receptor
or by its ability to block sodium channels.
UA/OP/05
Validation of RP‑HPLC methods for estimation
of Lupeol, Wedelolactone and β ‑Asarone from
Symplocos racemosa, Eclipta Alba, Acorus calamus
and their marketed Ayurvedic and herbal formulations
Sunita Shailajan, Sasikumar Menon1, Gauri Swar, Dipti Singh
Herbal Research Laboratory, Ramnarain Ruia College, Matunga,
Mumbai, 1Therapeutic Drug Monitoring Laboratory, Sion, Mumbai, India
E-mail: dips26098@gmail.com
Background: Symplocos racemosa Roxb. (Symplocaceae,
bark), Eclipta alba Hassk. (Asteraceae, whole plant) and
Acorus calamus Linn. (Araceae, rhizomes) have been
used traditionally in the management of a wide range
of ailments and are important component of Ayurvedic
system of medicine. Lupeol, wedelolactone and β‑asarone
are the active phyto constituents of the above mentioned
plants. Various Ayurvedic formulations like Lodharasava,
Pusyanuga Churna, Mahabhringaraj Taila, Mahavat
Vidhvansan Rasa, Medhya Rasayana, etc., containing these
plants are commercially available. Objective: The main
objective of this study is marker‑based standardization of
these natural resources and their commercially available
Ayurvedic formulations using validated RP‑HPLC technique.
Material and Methods: Separation of biomarkers lupeol,
wedelolactone and β‑asarone was achieved using
RP‑HPLC‑PDA technique and the methods were validated as
per ICH guidelines. As an application of method, estimation
of markers was carried out from Ayurvedic formulations
and samples from different environments. Results: A good
linear relationship was obtained for the calibration plots
of lupeol, wedelolactone and β‑asarone. Methods were
found sensitive for estimation of lupeol, wedelolactone and
β‑asarone (LOD and LOQ values were nearly 0.5 and 1.0 μg/
ml respectively). The validated method was found to be
simple, accurate, rugged and reproducible. These methods
can be recommended for quality assurance and marker‑based
standardization of various plants, their parts and formulations
containing these markers. Conclusion: These methods can
be extended into quantification of plant phyto chemicals,
checking authenticity of herbal formulations and facilitating
routine quality control analysis of commercial Ayurvedic
formulations.
UA/OP/06
Macaranga peltata ‑ Potential Wound Healing Agent
Minakshi Nehete, Pratima Tatke
Department of Pharmaceutical Chemistry, CU Shah College of
Pharmacy, SNDT Women’s University, Santacruz, Mumbai, India
E-mail: minakshi1277@yahoo.co.in
Background: Macaranga peltata is commonly known as
Chandwar. In tribal medicine, gum powder of Macaranga
peltata bark is used to join fractured bones. In a preliminary
study in our laboratory, it is shown to possess antioxidant
and wound healing activities. There are no literature reports
with respect to scientific evaluation on Macaranga peltata.
Objective: Objective of present study was to investigate in
detail the antioxidant, antimicrobial and wound healing activity
of Macaranga peltata. Materials and Methods: The powdered
bark of Macaranga peltata was extracted with various
solvents using soxhlet apparatus. Prepared extracts were
screened by determining total phenolic content (TPC) using
Folin‑Ciocalteu phenol reagent method. Methanol extract
showing highest TPC was evaluated for detailed antioxidant
and antimicrobial activity against wound pathogens. A topical
gel was formulated by incorporating methanol extract in
suitable gelling agent. Wound healing activity of this gel
was studied by using excision and incision wound models.
Betadine was used as a standard. Results: The methanolic
extract showed potent antioxidant and antimicrobial activity.
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Abstracts
These abstracts were presented at the Update Ayurveda 2014 at Mumbai, Maharashtra, India. The abstracts were screened
by the scientific committee of Update Ayurveda 2014 and are not peer reviewed by J-AIM. © Update Ayurveda 2014.
7
The statistical analysis indicated that wounds treated with gel
containing 1% w/w methanolic extract exhibited significant
increase in tensile strength (P < 0.001) as compared to
control. The epithelialization period was found to be less
as compared to control. The results demonstrated that
Macaranga peltata displayed promising wound healing
activity. Conclusion: A topical gel formulation containing
methanol extract of bark of Macaranga peltata was prepared
and evaluated. The gel exhibited excellent wound healing
activity on fresh wound in rats.
UA/OP/07
Cytochrome P450 inhibition potential of Trigonella
(Fenugreek), Trachyspermum (Ajwaine) and Nigella
Sativa (Kalonji) in rat liver microsomes
Dipali Mendhe, Madhav Singh Seervi, Sadhana Sathaye
Pharmacology lab II, Institute of Chemical Technology,
Matunga, Mumbai, India
E-mail: dips.mendhe@gmail.com
Background: The use of natural products as herbal medicines
has increased over the last decade. Such widespread
use of herbal medicinal products throughout the world
has raised serious questions concerning the quality,
safety and efficacy of these products. Literature survey
suggested the crucial role of Cytochrome P450 in drug
metabolism and drug‑herb interactions as approximately
80% of the drugs are metabolized by the same. Trigonella
foenum (Fenugreek), Trachyspermum copticum (Ajwain) and
Nigella sativa (Kalonji) are very commonly used Indian spices
and widely used in traditional herbal medicines as dietary
supplements. Objective: The present study was undertaken
to investigate the effects of methanolic extracts of the
above mentioned herbs on CYP1A2, CYP2D6 and CYP3A4
in rat liver microsomes in vitro. Materials and Methods: Rat
liver microsomes were prepared by calcium aggregation
method. Microsomes were then characterized for the ethoxy
resorufin‑O‑deethylase, dextromethorphan demethylase and
testosterone hydroxylase activities. Inhibition potential of
Trigonella foenum, Trachyspermum copticum and Nigella
sativa extract were evaluated. Results: The result suggested
that Trigonella, Trachyspermum and Nigella extract did not
show any inhibition towards CYP1A2, CYP2D6 and CYP3A4.
The IC50 Values for Trigonella, Trachyspermum and Nigella
were found to be 515, 188 and 136 μg/ml for CYP1A2,
827,107 and 47.4 μg/ml for CYP2D6 and >500, >500
and >500 for CYP3A4 respectively. Conclusion: It can
be concluded that the above mentioned herbs are safe to
use along with other drugs as they exhibited no significant
inhibition towards CYP1A2, CYP2D6 and CYP3A4.
UA/OP/08
PCOS‑Merging Ayurvedic and Allopathic Management
Jayshree S. Dawane, Vijaya A. Pandit, Kapil S.Khade,
Sonali P. Suryawanshi, Asmita Wele1, Madhuri Dalvi1,
Supriya Bhalerao2
Department of Pharmacology, BVDU Medical College, Pune,
1Department of Rasashastra and Bhaishajyakalpanavigyan,
BVU College of Ayurveda, Pune, 2IRSHA, BVU Pune, India
E-mail: jayshreedawane@gmail.com
Background: Polycystic ovarian syndrome (PCOS) is a
complex endocrine disorder characterized by elevated levels
of androgens, insulin resistance, anovulation, amenorrhoea
or oligomenorrhoea, obesity and Polycystic ovaries.
Objective: To evaluate efficacy of Ayurvedic formulations
Nishamalaki and Gugul in PCOS induced in wistar rats.
Material and Method: Nishamalaki, was prepared from
powder of Curcuma longa and fresh juice Emblica officinalis
according to Ayurvedic literature and administered with
honey. PCOS was induced in female rat’s with1mg/kg
body weight letrozole for 28 days. Vaginal smears collected
daily from all the groups and studied for changes in estrous
cycle. In one group i.e. Group I (Control), rats were treated
only with Saline, Letrozole treated 30 female rats were
divided into 5 groups Group II was (Control) PCOS Induced
(No treatment), Group III was Clomiphene citrate, Group IV
was Nishamalaki, Group V was Nishamalaki and Metfromin
and Group VI was Nishamalaki, Metfromin and Gugul and
treatment was given orally according to groups for 42 days.
Body weight, food intake, insulin, blood glucose levels was
checked before and after treatment. Results: PCOS induced
rat showed increase in body weight and insulin levels. Lipid
profile was altered in these animals. Nishamalaki reduced
serum cholesterol (P < 0.05), triglycerides (P < 0.01) and
LDL levels (P < 0.01). The body weight loss (P < 0.001)
was more significant with Nishamalaki treatment. These
findings demonstrate that levels of serum glucose,
cholesterol and triglycerides decreased significantly in PCOS
treated rats with Nishamalaki as compared to Clomiphene
citrate. Conclusion: Nishamalaki is the most promising drug
in PCOS, it has reduced weight, normalised lipid profile and
oestrus cycles.
UA/OP/09
Protective action of Carissa Carandas in Gentamicin
induced nephropathy via attenuation of oxidative
stress
Jayesh B. Dhodi, Deepavali R. Thanekar, Snehal N. Mestry,
Archana R. Juvekar.
Institute of Chemical Technology, Department of Pharmaceutical
Sciences and Technology, Matunga, Mumbai, India
E-mail: jayeshdhodi2@gmail.com
Background: Oxidative stress is reported to play a
pivotal role in the pathogenesis of nephropathy, one of
the co‑morbidities associated with diabetes. A previous
study carried out on methanolic extract of fruits of
Carissa carandas (MCCF) demonstrated the ameliorative
effects in diabetes induced nephropathy in rats. Thus,
in order to understand the mechanism of action of
MCCF, it was evaluated in gentamicin (GM) induced
nephropathy in rats. Gentamicin induces acute kidney
injury through oxidative damage produced by free
radical generation. Objective: To evaluate the effect
of MCCF on oxidative stress in gentamicin induced
nephropathy. Materials and Methods: Male Sprague
Dawley rats were divided into five groups; Group I ‑ normal
control (received distilled water, p.o.), Group II ‑ negative
control (gentamicin 80 mg/kg i.m., 8 days), group III,
IV and V were administered100, 200 and 400 mg/kg
MCCF p.o. respectively along with gentamicin 80 mg/kg
[Downloaded free from https://www.jaim.in on Monday, February 15, 2016, IP: 115.112.118.203]
Abstracts
These abstracts were presented at the Update Ayurveda 2014 at Mumbai, Maharashtra, India. The abstracts were screened
by the scientific committee of Update Ayurveda 2014 and are not peer reviewed by J-AIM. © Update Ayurveda 2014.
8
i.m. for 8 days respectively. Biomarkers of nephropathy
were assessed in serum and urine. Oxidative stress
parameters, superoxide dismutase, catalase, reduced
glutathione and lipid peroxidation were evaluated in
kidney homogenates. The extent of kidney damage was
analyzed by histo‑pathological study. Results: MCCF
significantly improved the altered oxidative stress levels
in gentamicin induced nephropathy in a dose dependent
manner as compared to negative control. The progression
of nephropathy was markedly inhibited as demonstrated by
decreased levels of biomarkers in serum and urine and the
histo‑pathological analysis. Conclusion: MCCF ameliorated
the development of nephropathy by suppressing oxidative
stress in gentamicin induced nephropathy in rats.
UA/OP/10
Saraca Indica fraction prevents cataract development
and progression in Streptozotocin‑induced diabetic
rats: an aldose reductase inhibitory activity
Sadhana Sathaye, Gauresh Somani
Pharmacology Research Laboratory‑II, Department of
Pharmaceutical Sciences and Tech, Institute of Chemical
Technology Mumbai, India
E-mail: sadhanasathaye@hotmail.com
Background: Cataract, characterized by cloudiness or
opacification of the eye lens is the leading cause of blindness
in developed and developing countries. Diabetes is one of the
prime causes for cataract development. Objective: The present
study was designed to investigate the effect of bioactive
fraction of Saraca Indica flowers (SIF) extract on development
and progression in streptozotocin (STZ) induced diabetic
rats. Materials and Methods: The inhibitory effect of SIF
extract and its fraction was tested for inhibition against rat
lens aldose reductase (AR). Furthermore, active fraction was
investigated against high glucose induced opacification of
lens in lens culture and STZ induced diabetic cataract in rats.
Identification of the bioactive components was attempted
using different analytical techniques such as HPTLC, HPLC,
LC‑MS and GCMS. Docking pattern and binding interactions
of the bioactive components gave valuable insights on
their mechanism of action. Results: Ethyl acetate fraction
produced maximum inhibition of aldose reductase activity.
Treatment with SIF fraction at a concentration of 1 mg/ml
lowered lens opacity in 72 hrs. In vivo experiments were
performed with STZ induced diabetic rats. Prolonged
treatment with ethyl acetate extract of SIF to STZ‑induced
diabetic rats decreased the blood glucose levels and inhibited
the AR activity and delayed cataract progression in dose
dependent manner. Conclusion: The present study shows
that ethyl acetate extract of SIF has pharmacologically active
components with a potential to inhibit rat lens AR activity.
UA/OP/11
Assessment of antimicrobial properties of hydro
alcoholic extract of Morinda citrifolia
Rajesh Kumar Suman, Ipseeta Ray Mohanty, Vijay Mane,
Y. A. Deshmukh, A. D. Urhekar
MGM Medical College, Kamothe, Navi Mumbai, India
E-mail: rajeshsuman2043@gmail.com
Background: Morinda citrifolia is a versatile medicinal
plant with a broad spectrum of pharmacological activities
and traditionally being used for the treatment of various
diseases. Increasing emergence of resistance to the currently
available antibiotic has necessitated continuing search for
new antimicrobial compound. Hence, the present work
was designed to investigate the antimicrobial activity
of extract of Morinda citrifolia. Objective: To evaluate
antimicrobial activity of Hydro alcoholic fruit extract of
Morinda citrifolia against E. coli, Psedomonas aeroginosa
and Acinatobactor. Material and Methods: The Hydro
alcoholic extract of Morinda citrifolia (Fruit) was purchased
from Sanat Pharmaceutical, New Delhi. The extract was
tested against standard strains and clinical isolate of
E. coli, Pseudomonas aeroginosa and Acinatobactor by
Agar well diffusion technique using Muller Hinton agar.
Different concentration of test drug 1mg, 2 mg, 4 mg and
8 mg were tested. Cefotaxime was used as control drug.
Results: The Hydro alcoholic extract of Morinda citrifolia at
concentration of 1 mg, 2 mg, 4 mg and 8 mg were tested
against three bacteria. E. coli (ATCC 25922) and E. coli
from clinical isolate (Urine), were significant sensitive with
test drug as compared with control drug. The Pseudomonas
aeroginosa (ATCC 27853) and Pseudomonas aeroginosa of
clinical isolate (Pus) were also significant sensitive by Test
drug. Against, Acinatobactor from clinical isolate (Tracheal
Secretion), the test drug did not demonstrate antimicrobial
activity at 1 mg, while it showed significant activity at Conc.
of 2 mg, 4 mg and 8 mg as compared with control drug.
Conclusion: The herb Morinda citrifolia was found to have
significant antimicrobial properties against test bacteria.
UA/OP/12
Anti‑epileptogenic and anti oxidant effect of Borneol:
assessment in pentylenetetrazole induced kindling
model of epilepsy
Rufi Tambe, Pankaj Jain, Sadhana Sathaye
Pharmacology Lab‑II, Department of Pharmaceutical Sciences and
Technology, Institute of Chemical Technology, Matunga, Mumbai, India
Email: rufitambe@gmail.com
Background: Borneol, a bicyclic monoterpene has been
found to exhibit a positive GABA modulatory activity at
human recombinant GABA receptors. Borneol showed
neuroprotection on cortical neurons against oxygen‑glucose
deprivation/reperfusion in vitro and exhibited anti‑inflammatory
and anti‑oxidant activity on cortical neurons. Objective: The
present study was aimed at investigating the anti‑epileptogenic
effect of Borneol in the pentylenetetrazole (PTZ) induced
kindling model along with its ability to suppress oxidative
stress and neuro‑inflammatory marker glial fibrillary acidic
protein (GFAP). Materials and Methods: Borneol (5, 10,
25 mg/kg, i.p.) was evaluated on the course of kindling
followed by estimation of oxidative stress markers like
superoxide dismutase (SOD), reduced glutathione (GSH),
catalase (CAT) and lipid peroxidation (MDA) in PTZ kindled
mice. GFAP was also evaluated at the end of the study
using immunohistochemistry. Results: The results showed
that treatment with Borneol significantly decreased the PTZ
induced seizure score. The biochemical alterations induced by
PTZ were ameliorated in Borneol treated animals which was
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Abstracts
These abstracts were presented at the Update Ayurveda 2014 at Mumbai, Maharashtra, India. The abstracts were screened
by the scientific committee of Update Ayurveda 2014 and are not peer reviewed by J-AIM. © Update Ayurveda 2014.
9
indicated by decreased malondialdehyde levels and increased
glutathione, catalase and superoxide dismutase levels. It
also alleviated the levels of GFAP as manifested by less
immunostaining for the same. Conclusion: The above results
are suggestive of the anti‑epileptogenic potential of Borneol
and it being a promising candidate in the treatment of epilepsy.
UA/OP/13
Punica Granatum Fruit peel: A potential candidate for
holistic treatment in inflammatory disorder of Intestine
Deepali Mahajan, Usha Satyapal, Pratima Tatke,
Vikram Naharvar
Department of Pharmaceutical Chemistry, C. U. Shah College of
Pharmacy, S.N.D.T Women’s University, Santacruz, Mumbai, India
E-mail: deepali.mahajan@gmail.com
Background: Punica granatum fruit, commonly called
pomegranate belongs to family Lythracea. In Ayurveda
its peels are used in treatment of diarrhea and dysentery.
The peels are rich in polyphenols such as tannins and
flavonoids. Objective: The objective of the present is to
study the effects of methanolic extract of pomegranate
peels (ME‑PP) in diarrhea, intestinal inflammation and pain.
Materials and Methods: The anti diarrheal effect of ME‑PP
was investigated by measuring inhibitory action on diarrhea
and intestinal fluid accumulation induced by castor oil; and
intestinal motility in rats. Effectiveness against intestinal
inflammation induced by intra rectal instillation of TNBS
was assessed in mice. The extent of colonic injury was
evaluated by colonic macroscopic damage scores, weight/
length ratio, histopathology; diarrhea score, feed intake and
bodyweight of mice. The colonic inflammatory response
was assessed by TNFα and IL1‑β; and oxidative stress
by malondialdehyde (MDA) levels. The analgesic effects
were evaluated by writhing test in mice. Results: ME‑PP
showed dose dependent effect against diarrhea, intestinal
inflammation and pain. Administration of ME‑PP delayed
the onset of diarrhea, reduced the intestinal motility, and
decreased the intestinal fluid accumulation. The colonic
mucosal injury and inflammation was significantly reduced
by ME‑PP as evidenced by reduced colonic damage, weight/
length, cytokine levels (TNF‑α and IL1‑β) and MDA levels.
Analgesic effect of ME‑PP was demonstrated by reduction
in the writhing which can be correlated to inflammatory
pain associated with intestinal inflammation. Conclusions:
ME‑PP can be a potential holistic treatment for treating
inflammatory disorders of intestine and relieving the
associated symptoms such as diarrhea and pain.
UA/OP/14
Marker‑based standardization of two ayurvedic
formulations: Arka vati and Lavangadi vati
Sunita Shailajan, Sasikumar Menon, Mayuresh Joshi,
Bhavesh Tiwari
Herbal Research Lab, Ramnarain Ruia College, Matunga, Mumbai,
E-mail: m2j5288@gmail.com
Background: Arka Vati (AV) and Lavangadi Vati (LV) are
traditional Ayurvedic formulations used in the treatment of
indigestion, digestive impairments caused by Kapha Dosha,
allergic respiratory conditions, etc. Though the formula
composition and therapeutic claims of these formulations
are part of Ayurvedic Formulary of India, validated scientific
methods for its quality and safety evaluation are yet to
be documented. Objective: Present study was aimed to
carry out marker‑based standardization of AV and LV using
validated HPTLC method. Material and Methods: Standardized
operating procedure for the preparation of AV and LV were
developed in accordance with Ayurvedic Formulary of India.
Physicochemical and preliminary phyto chemical evaluation
was carried out as per reported methods. Chromatographic
evaluation of these formulations was performed using validated
HPTLC method in terms of their piperine content (piperine a
major component of common ingredient Marica). As an
application of this method, in‑house formulations were
compared with the formulations available in the market.
Safety of the formulation was evaluated in mice as per OECD
guideline no. 420. Results: SOPs’ of both the formulations
has been established. Content of piperine was determined
from both the formulations using a simple, rapid and accurate
HPTLC method. Significant variation in the piperine content
was observed in various AV and LV formulations. Conclusion:
The results of quality control parameters obtained after
scientific evaluation of AV and LV can be used as reference
standard for quality control/assurance in laboratories of
pharmaceutical firms in order to have a proper quality check
over their preparation and processing.
UA/OP/15
Hridayavarga Aushadhi‑Carissa carandas: Evaluation
of its antihypertensive activity
Kalyani H. Barve, Tejas Rami
Shobhaben Pratapbhai Patel‑ School of Pharmacy and Technology
Management, SVKM’s NMIMS, Mumbai, India
E-mail: kalyani.barve@nmims.edu
Background: Carissa congesta belonging to Apocynaceae, is
known as karamarda (Sanskrit). It is found throughout India.
The root is credited with stomachic, anti diarrheal, anthelmintic
properties and contains salicylic acid, lignin and sesquiterpenes:
carissone and carindone. However, in Ayurvedic and Unani
system of medicine it is reported to have an effect on the
cardiovascular system, but no scientific evidence exists
for the same. Even the Charaka samhita mentions this as
Hridayavarga class. Objective: To evaluate Phyto‑chemical
and pharmacological comparison of root and bark extract
and Co‑relation of therapeutic activity with phyto‑constituent
of bark and root. Materials and Methods: Alcoholic extract
of bark (BE) and root (RE) was prepared. The extracts were
screened for detection of different phyto‑constituents and
subjected to fingerprinting by TLC. The proposed active
phyto‑constituents of the extracts were evaluated by
molinspiration software to predict the bioactivity and were
then evaluated at 250mg/kg, to check the antihypertensive
effect in cadmium chloride induced hypertensive animals.
Results: The extracts showed the presence of steroids,
glycosides, tannins and alkaloids. The BE showed a blood
pressure of 94.97 + 11.94 mm Hg whereas the RE showed a
blood pressure 67.33 + 8.79 mm Hg against 115.88 + 4.88
mm Hg pressure in hypertensive rats. Carrisone was the major
phyto‑constituent as revealed by the fingerprinting of BE and
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Abstracts
These abstracts were presented at the Update Ayurveda 2014 at Mumbai, Maharashtra, India. The abstracts were screened
by the scientific committee of Update Ayurveda 2014 and are not peer reviewed by J-AIM. © Update Ayurveda 2014.
10
RE.It further showed nuclear receptor binding activity and
enzyme inhibitory activity. Conclusion: The study proves that
the BE and RE of Carissa carandas possesses antihypertensive
activity. However, further investigation are being undertaken
to find the exact mechanism.
UA/OP/16
An upshayatmaka trial of Shreephaladi Vati and
Marichyadi Churna in Grahani dosha
Anita Meghwal, Nadira Khatoon, Pawankumar Godatwar,
Sevatkar Balkrishan, Sisir Kumar Mandal
Department of Roga and Vikriti Vijnana, National Institute of
Ayurveda, Amer Road, Jaipur, Rajasthan, India
E‑mail: anitameghwal09@gmail.com
Background: In the recent years, people are living with
sedentary lifestyle. Hence list of lifestyle generated
disorders are increasing day by day. Grahani dosha that is a
psychosomatic disorder caused by regular intake of junk food
that weakens the digestive fire and disharmony is produced
between the digestion and absorption of body. Objective: To
conduct an upshayatmaka trial of Ayurvedic drugs on grahani
patients. Materials and Methods: Total 60 randomly selected
cases, excluding dropouts were taken and assigned into two
groups (Group A and B). In Group A Shreephaladi Vati was
given in a dose of 2 Vati twice daily after food with Takra as
anupana and in Group B Marichyadi Churna was given in a
dose of 3 g twice daily after food with Takra as anupana. Total
duration of treatment was 2 months with the regular follow‑up
of an interval of 15 days for observing any side effect of
drugs. Results: Shreephaladi Vati on subjective parameters
showed statistically extremely significant (P < 0.0001)
improvement in Drava mala, muhur muh ur mala, Abdominal
pain/discomfort, mucous in stool, gas/flatulence, odor in
stool, arochaka and balakshaya, while Marichyadi Churna
showed statistically less significant improvement in mucous
in stool, gas/flatulence and balakshaya. Conclusion: Both
Shreephaladi Vati and Marichyadi Churna significantly reduced
the symptoms of grahani dosha, although Shreephaladi Vati
was much more effective in reducing the subjective variables
of Grahani Dosha.
UA/OP/17
Studies on Pravala Moola and Shakha Bhasma and
Pisti w.s.r. to pharmaco‑kinetics
Puja Sansare, K. Shankar Rao
Department of Rasashastra, National Institute of Ayurveda, Jaipur,
Rajasthan, India E‑mail: pujasansare@rediffmail.com
Background: Pharmacokinetics is a branch of pharmacology
that deals with the factors that establishes a relationship
between drug dosage and variation of drug concentration at
the site of action. Pravala is a well‑known drug in Ayurvedic
literature since ages, and it has been used in various forms such
as Bhasma and Pisti. For the present study, Pravala Bhasma
and Pisti of both Shakha and Moola have been evaluated for
its absorption and efficacy through the pharmacokinetics.
Objective: To assess the absorption pattern of all the Pravala
samples in serum at certain intervals. Materials and Methods:
The pharmacokinetics study was conducted on dogs so as
to determine the different plasma concentration of calcium
after oral administration of test drugs. The amount of calcium
present in the serum before and after administration of
samples was assessed. Further assessment was done at an
interval of ½, 1st, 2nd, 3rd, 4th, 5th, 6th, 7th and 8th h. Results:
The results showed that initially serum calcium level started
increasing after ½ h of drug administration. The peak level of
serum calcium reached at 3rd h and it went down subsequently
till 6th h. At 7th and 8th h, the serum calcium level reached
baseline. Conclusion: Overall the present study suggests
that the Pravala Moola bhasma showed comparatively better
results in increasing serum calcium levels.
UA/OP/18
Clinical evaluation of the role of tablet cardicare and
Basti treatment in the management of coronary artery
disease (Hridyaroga)
Hemant Narwade, R. K. Joshi
Department of Kayachikitsa, National Institute of Ayurveda, Jaipur,
Rajasthan, India
E‑mail: hemantsnarwade@gmail.com
Background: In India, approximately 70 million people suffer
from coronary artery disease (CAD). CAD can be correlated
with Hridyaroga, which has been described in Ayurvedic
texts. WHO considered it as a modern epidemic as it is fourth
leading cause of death. As allopathic treatment of CAD need
group of medicines having a lot of side effects, so there is a
need of some herbal formulation. Objective: Present study
was done to assess the efficacy of herbal formulation tablet
cardicare and Basti treatment in comparison to standard
drug diltiazem (tablet dilzem) on the basis of subjective
and objective criteria. Materials and Methods: Totally,
30 registered patients were divided into three groups
(each containing 10 patients) Group 1 patients received tablet
dilzem (diltiazem hydrochloride) 30 mg TDS for 30 days.
Group 2 patients received tablet cardicare 2 tablets TDS
with lukewarm water for 30 days and Group 3 patients
received tablet Cardicare for 30 days and Basti (kalakrama)
with Dashmula taila and Dashmula kwatha. Patients were
followed up after 15 and 30 days of treatment. Result:
Group 2 and Group 3 patients showed highly significant result
and Group 1 patient showed significant result in symptoms
of CAD “grading scale” provided by “Canadian cardiovascular
society” with changes in lipid profile, electrocardiogram, and
computerized treadmill test. Conclusion: The trial drug tablet.
Cardicare having cardio‑protective and anti‑angina action
along with Basti treatment showed significant results in the
management of CAD (Hridroga) as seen with its effect on
various scientific parameters.
UA/OP/19
Pharmacological evaluation of antipyretic and
analgesic activity of Pippali (Piper longum linn.) fruit
lavigated with cow’s urine
Rabinarayan Acharya, Kapil Lathiya, Rasika Kolhe,
Mukesh Nariya
Department of Dravyaguna, IPGT and RA, GAU, Jamnagar,
Gujarat, India
E‑mail: drrnacharya@gmail.com
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Abstracts
These abstracts were presented at the Update Ayurveda 2014 at Mumbai, Maharashtra, India. The abstracts were screened
by the scientific committee of Update Ayurveda 2014 and are not peer reviewed by J-AIM. © Update Ayurveda 2014.
11
Background: In Ayurveda, Pippali (Piper longum L) and
Gomutra have significant therapeutic values in many
diseases. Bhavana (lavigation) is a procedure intended
to increase the efficacy of the drug or to get the desired
effect. In folklore practice, Gomutra Bhavita Pippali (Pippali
lavigated with cow’s urine) is used in the management
of fever. The growing side effects of the synthetic drugs
necessitate the research for the evaluation of safe, effective
and fast‑acting analgesic and antipyretic herbal remedies.
Objective: To evaluate the antipyretic and analgesic effect
of Pippali fruits lavigated seven times with cow’s urine in
the experimental model. Materials and Methods: For each
study, Charles Foster rats of both the sex were divided
into three groups of six animals each. For anti‑pyretic
and analgesic effect; Brewer’s yeast induced pyrexia,
and Tail flick method was adopted respectively. The test
drug was administered orally at a dose of 540 mg/kg
and the activities were compared with standard control
in both models. Result: In antipyretic study, 1 h after
Brewer’s yeast administration in rats, standard control
paracetamol group produced significant decrease (110%)
and Gomutra Bhavita Pippali produced (96%) decrease in
rectal temperature but insignificant (48.9%) effect was
seen in Gomutra Bhavita Pippali after 3 h in comparison
to control group. Gomutrabhavita Pippali produced
non‑significant increase in tail flick response in comparison
to control group, as well as initial values in evaluation of
its analgesic effect. Conclusion: Gomutra Bhavita Pippal
produced significant antipyretic and moderate analgesic
activity.
UA/OP/20
A preliminary phytochemical evaluation of leaf and
seed of Ipoemia pentaphylla linn
Minutee Patel, Chetan Kamble, Sudipta Roy,
Anagha Ranade, V. J. Shukla, R.N. Acharya
Department of Dravyguna, IPGT and RA, GAU, Jamnagar,
Gujarat, India
E‑mail: ckkamble89@gmail.com
Background: The authentic identification and standardization
of Ayurvedic drugs is a very essential part to assess the
genuinity of the crude drugs. Folklore claims, an integral
part of traditional systems of medicine, are needed to be
validated thoroughly. The phytochemical analysis of any
drug is a primary step toward its authentication. Different
parts of Ipoemia pentaphylla Linn., a folklore medicinal
plant, not reported for its chemical nature, is reported for
its blood purifier, anti‑inflammatory actions. Objective: To
assess the analytical parameters of I. pentaphylla leaf
and seed. Materials and Methods: The study included
primary organoleptic character evaluation followed by
physicochemical analysis constituting determination of loss
on drying, total ash, alcohol soluble extractive value, pH,
etc. Qualitative tests were done to identify the presence of
various chemical constituents followed by quantitative
estimation of protein and HPTLC study. All the studies
were carried out following standard laboratory parameters.
Results: The study revealed the presence of higher moisture
content in the seeds than in the leaf Ash values of seed
and leaf were found to be 26.31%w/w and 16.43% w/w
at 4500C. Water‑soluble extractive value of leaf was
higher while alcohol soluble extractive value was higher in
seeds. Preliminary qualitative tests revealed presence of
alkaloids, tannin, steroids, flavonoids, carbohydrates, etc.,
in leaf and amino acids and protein in seed. Quantitative
protein estimation and HPTLC study were also performed.
Conclusion: The present observations may be considered as
a standard parameter for preliminary phytochemical values
of I. pentaphylla Linn.
UA/OP/21
Krimi chiktsa in Amlapitta and its significance ‑ a statistical
analysis
Neha Sharma, Sameer Naik
Department of Kayachikitsa, KLE University’s Shri BMK Ayurveda
Mahavidyalaya, Shahapur, Belagaum, Karnataka, India
E‑mail: dr.nehasharma24@gmail.com
Background: In Ayurveda, Amlapitta is a disorder considered
for gastric irritation, abdominal pain, etc. Amlapitta is
a conditional disorder that is considered as precursor
of Grahani roga and for grahani roga, Krimi chiktsa is
mentioned. Objective: To analyze whether the Krimi
chikitsa can show a significant result in Amlapitta or not.
Materials and Methods: A prospective study was carried out
in individuals having chief complaint of Amlapitta. Totally,
105 subjects were randomly selected strictly on the basis
of inclusion and exclusion criteria who visited the OPD in
the year 2013 and 2014 at our institute. Among them, 32
belonged to vata predominant prakriti (30.47%), 53 pitta
predominant prakriti (50.47%), 20 kapha predominant
prakriti (19.04%). All patients were treated with Krimikuthara
rasa and Vidangarista. Results: It was observed that the
chief symptoms of Amlapitta that is, burning sensation,
abdominal pain, arochaka were reduced up to 30–40%.
Conclusion: The study reveals the beneficiary role of Krimi
Chikitsa in Amlapitta patients.
UA/OP/22
Wound healing activity of standardized methanolic
extract of Randia dometorum (rubiacae)
Chhaya Gadgoli, Priyanka Gosavi
Department of Pharmacognosy, Saraswathi Vidya Bhavan’s College
of Pharmacy, Dombivli, Mumbai, Maharashtra, India
E‑mail: chhayahg@rediffmail.com
Background: Randia dometorum fruits are commonly called
as Madana, utilized in folklore medicine for treatment
of ulcers, inflammation, wounds and skin diseases.
Objective: The present study was carried out with the aim
to evaluate the wound healing activity of methanolic extract
of fruits of R. dometorum. Materials and Methods: The fruits
were extracted successively with methanol and water. The
methanolic extract was incorporated in simple ointment base
in the concentration of 1 and 2% w/w and evaluated for the
wound healing activity in rats using excision and incision
wound models. The monitoring of wound healing process
was done through determination of parameters viz., period of
epithelization and hydroxyproline content in eschar in excision
wound model, while in case of incision wound model the tensile
[Downloaded free from https://www.jaim.in on Monday, February 15, 2016, IP: 115.112.118.203]
Abstracts
These abstracts were presented at the Update Ayurveda 2014 at Mumbai, Maharashtra, India. The abstracts were screened
by the scientific committee of Update Ayurveda 2014 and are not peer reviewed by J-AIM. © Update Ayurveda 2014.
12
strength of newly formed skin was determined. A triterpenoldal
aglycon RD1 isolated from the metahnolic extract was utilized
for the standardization of the extract. Results: The methanolic
extract was found to contain 0.45% w/w of RD1 that was
characterized to be oleanolic acid. The methanolic extract
in the concentration of 2% w/w exhibited good wound
healing activity as significant (P < 0.001) reduction in the
epithelization period and significant (P < 0.001) increase
in the hydroxyproline content and the tensile strength, was
observed as compared to the control group treated with the
simple ointment base. Conclusion: Methanolic extract of the
fruits of R. dometorum has good potential as wound healing
agent and is rich in saponins.
UA/OP/23
Gastro protective effect and biomarker‑based
pharmacokinetic profile of an Ayurvedic formulation:
Bilvadileha
Sunita Shailajan, Archana Matani
Herbal Research Lab, Ramnarain Ruia College, Matunga, Mumbai,
Maharashtra, India
E‑mail: sampatarchana@yahoo.com
Background: The high risk of developing gastric ulcers by
non‑steroidal anti‑inflammatory drugs has affected their
common application in the treatment of various inflammatory
disorders. Several indigenous plants and formulations
have been described in Indian systems of medicine for
such symptoms. Bilvadileha is an Ayurvedic semi solid
formulation prescribed for the treatment of digestive
impairment, tastelessness, emesis and excessive salivation.
Objective: Though composition and therapeutic claims of
Bilvadileha are part of the Ayurvedic Formulary of India, the
scientific validation of its pharmacological profile is yet to
be documented. Materials and Methods: Chromatographic
standardization was carried out using HPTLC, which included
the determination of piperine content in Bilvadileha along with
the evaluation of its stability samples for different storage
periods. Acute toxicity studies were conducted in mice as
per OECD guidelines No. 420. Evaluation of the preventive
effect of Bilvadileha on indomethacin induced (30 mg/kg p.o.)
experimental gastric ulcer in rats (180–200 g) was carried
out and compared with that of synthetic anti‑ulcer drug
Omeprazole (10 mg/kg). Pharmacokinetics of piperine from
Bilvadileha after oral administration to rabbits was evaluated
using validated high‑performance liquid chromatography
method. Results: Piperine content in Bilvadileha was found to
be 0.72 ± 0.02 mg/g using HPTLC. Safety of the formulation
was affirmed in mice at 2 g/kg. Aqueous slurry of Bilvadileha
prevented ulcer formation in indomethacin‑induced ulcer
model. Piperine was detected in rabbit plasma with Cmax and
Tmax of 79.21 ± 1.24 ng/ml and 0.75 h respectively post
administration of Bilvadileha. Conclusion: The current work
demonstrates modern approach toward standardization of
traditional Ayurvedic formulation Bilvadileha.
UA/OP/24
Correlation between Mamsa sarata and muscle
strength
Jitendra Rajput, R. R. Deshpande
Department of Kriya Shareer, College of Ayurved and Research
Centre, Nigdi, Pune, Maharashtra, India
E‑mail: dr.jitendra8rajput@gmail.com
Background: In the Dashvidha pariksha of Ayurveda, it
has stated that Saar pariksha is one of the important
investigation that is, examination for the strength. To
understand the quantitative and qualitative strength of
body, Ayurveda has specifically explained the Dhatu Sarata
concept. Dhatu quality is important for immunity aspect and
body strength. Ayurveda says Mamsa Sara person has a
good quality Bala. Bala can be translated in many ways as
muscle strength or stamina or muscle flexibility. Strength
of the body can be explained as “Bala”. So, the topic has
been selected to find out the relation of Mamsa Sarata
and “Muscle strength” by grip dynamometer. The practical
applicability of this study will be the availability of objective
parameter for Bala, mentioned in Mamsa Sarata examination.
Materials and Methods: Totally, 50 healthy volunteers were
selected. Mamsa Sarata was taken out with the help of the
conventional method (questionnaire). Grip dynamometer
was used for muscle strength measurement. Both the
readings were co‑related with each other. Results: While
doing the study, it was noticed that muscle strength could
be co‑related with mamsa sarata. Uttam mamsa sara
people have good muscle strength while heen mamsa
sarata person have less muscle strength. Conclusion: So
grip dynamometer can be used instead of mamsa sarata
questionnaire for taking out mamsa sarata and muscular
status of a person. This could be very useful and simple
and time‑saving process. This will help in updating Ayurveda
with the modern technology also.
UA/OP/25
Role of modern analytical validation of Ayurveda
Sidhipariksha for ayurvedic formulation w.s.r. to
Bhasma form
Atul Dnyandeo Sanap, J. M. Takalikar
Department of Rasashastra and Bhaishjya Kalpana,
Loknete Rajarambapu Patil Ayurvedic Medical College, Hospital,
Post Graduate Training and Research Center, Urun‑Islampur,
Sangli, Maharashtra, India
E‑mail: samap067@gmail.com
Background: As requirement of increased Ayurvedic drug
use, Ayurvedic pharmaceutical company has to establish
more production of Ayurvedic formulations (Drugs) within the
short period. These productions have affected the properties
and action of Ayurvedic medicine. Also, as the requirement
increases the genuine raw material is hard to get. In such
cases, substandard products may be manufactured specially
the Bhasmas. To hurry in supply the same they show low or
null medicinal effect. This produced bad image of Ayurveda
at national and international level. For avoiding these, there
is need to establish standards for it. Objective: To study
Ayurvedic Bhasma Pariksha in Ayurvedic view, to study
the modern analytical parameters for Ayurvedic Bhasma,
to correlate the Bhasma Pariksha and analytical parameters
for Ayurvedic Bhasma. Materials and Methods: The modern
analytical parameter like XRD, XRF, SEM‑EDX, AAS were
used and correlated with Ayurvedic Bhasma Pariksha such
as Varitaratva, Apunarbhavatva, Rekhapurnatva, Mrutaloha,
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Abstracts
These abstracts were presented at the Update Ayurveda 2014 at Mumbai, Maharashtra, India. The abstracts were screened
by the scientific committee of Update Ayurveda 2014 and are not peer reviewed by J-AIM. © Update Ayurveda 2014.
13
Nirutha, Uttam Pariksha, Amla Pariksha. E.g. Vanga Bhasma
is in oxide form (SnO) with about 98% which can be
correlated with apunrbhavatva and practical size correlates
with Rekhapurnatva. Result: These show that the modern
analytical parameters tests are significant for Ayurvedic
Bhasma or vice‑a‑versa. Conclusion: There is a need to prove
and establish the standards of Ayurvedic Bhasma with the
help of modern parameters.
UA/OP/26
A randomized control trial of Arjuna and Agnimantha
on metabolic syndrome
Sachin Kumar, Pawankumar Godatwar, Himmat Lal Dhaker
Department of Rognidana, National Institute of Ayurveda, Jaipur,
Maharashtra, India.
E‑mail: drsachinrai1983@gmail.com
Background: Metabolic Syndrome has received increased
attention more and more in the past few years globally and
is the burning problem of today’s world. It is a set of risk
factors that includes abdominal obesity, insulin resistance,
hypertension and dyslipidemia. Objective: To evaluate
the efficacy of Arjuna churna and Agnimantha churna in
metabolic syndrome. Methods: Totally, 120 patients were
selected randomly and divided into two groups. Group A
patients were treated with Arjuna churna. Group B patients
were treated with Agnimantha churna. Chief symptoms
of metabolic syndrome along with prakriti and bala were
taken into consideration. The patients were diagnosed
for metabolic syndrome on the basis of criteria laid down
by International Diabetes Federation (2005). Results: In
Group B, chief symptoms of metabolic syndrome that
is, Chala sphika udara stana, Krichha vyavaya, Kshuda
vriddhi, Nidradhikya, Kshudra swasa, Alpa bala, Alasya,
Bahu mutrata, Hriddrava, Kosthabaddhata showed highly
significant improvement, while in Group A only Kshuda
vriddhi Svedadhikya and Kosthabaddhata showed significant
improvement. Biophysical parameters such as body
weight, body mass index, visceral fat, body fat, Basal
metabolic rate, Waist hip ratio, pulse rate, respiratory rate
and blood pressure (systolic and diastolic) were assessed
and significant results were obtained in both the groups.
Conclusions: Arjuna churna showed better results in Bhrama,
Shirah shoola, Hriddrava, while Agnimantha churna showed
better results in most of the other major signs and symptoms
of Metabolic syndrome.
UA/OP/27
Rosa damascena flower petals: A potential agent for
oxidative stress related disorders
Priti Patil, Pratima Tatke, S. Y. Gabhe1
Department of Pharmaceutical Chemistry, C. U. Shah College
of Pharmacy, S.N.D.T Women’s University, Santacruz, Mumbai,
1Department of Pharmaceutical Chemistry, Bharati Vidyapeeth
Deemed University, Poona College of Pharmacy, Erandwane, Pune,
Maharashtra, India
E‑mail: patilpriti25@gmail.com
Background: Rosa damascena mill (Rosaceae) is known
for its perfumery and cosmetic applications. Medicinal
properties of anti‑HIV, antibacterial and antioxidant activities
of R. damascena have been demonstrated. Rose petals
contain volatile oils, glycosides, phenolic such as flavonoids
that are responsible for its free radical scavenging activity.
Objective: The main objective of the study was to evaluate
the free radical scavenging activity of R. damascena flower
petals by various in‑vitro free radical scavenging assay
methods. Materials and Methods: Ethanol, chloroform,
acetone and aqueous extracts of fresh and dried Rose
petals were prepared and evaluated for the presence
of total phenolics in terms of Gallic Acid Equivalent.
Ethanol and acetone extract with high phenolic content
were selected for various in vitro free radical scavenging
assays using 2,2‑diphenyl‑1‑picrylhydrazyl (DPPH),
2,2′‑Azinobis [3‑ethylbenzothiazoline‑6‑sulfonate] (ABTS),
pyrogallol red bleach by peroxynitrite inhibition, xanthine
oxidase inhibition (XOI), superoxide scavenging and lipid
peroxidation assay. Results: All the extracts of fresh and
dried Rose petals were found to contain higher amount
of total phenolic calculated as mg/GAE/g. The amount
was highest in ethanol >acetone >aqueous >chloroform
extract. The ethanol extract of fresh and dried petals showed
lowest IC50 values than the acetone extract by DPPH, ABTS,
peroxynitrite inhibition, XOI, superoxide scavenging and lipid
peroxidation inhibition methods. Conclusion: Ethanol extracts
of fresh and dried Rose petals exhibited promising free radical
scavenging activity. These extracts have good potential in
the treatment of oxidative stress related disorders.
UA/OP/28
Pharmacognostic evaluation of Mimusops elengi L.
flowers: An important Ayurvedic medicinal plant
Deepti Gurjar, Sunita Shailajan
Herbal Research Lab, Ramnarain Ruia College, Matunga, Mumbai,
Maharashtra, India
E‑mail: deepsgurjar@gmail.com
Background: Mimusops elengi L. flowers (Sapotaceae,
Bakul) have been traditionally used in the history of medicine
and Ayurveda as a brain tonic, in the treatment of asthma
and for their different therapeutic activities like antiulcer,
wound healing, antioxidant, etc. Objective: The present
study was aimed to evaluate the pharmacognostic profile
of M. elengi flowers. Materials and Methods: M. elengi
flowers were subjected to evaluation of proximate and
physicochemical parameters. Phytochemical characterization
was carried out using validated HPTLC methods and gas
chromatography–mass spectrometry (GC‑MS) technique.
Safety profile of the plant was evaluated by carrying out
heavy metal analysis and acute oral toxicity study in rats
as per OECD guidelines no. 420. Results: Proximate and
physicochemical parameters of M. elengi flowers were
established. HPTLC analysis of M. elengi flowers revealed the
presence of some bioactive markers like β‑sitosterol, lupeol
and gallic acid whereas ursolic acid, quercetin and kaempferol
were not detected. GC‑MS analysis of flowers revealed the
presence of another bioactive marker eugenol too along
with presence of around 16 different compounds. M. elengi
flowers showed the level of heavy metals (As, Pb, Cd and Hg)
within the permissible limits. Results of the acute oral toxicity
study ensures adequate safety margin of M. elengi flowers at
2000 mg/kg in rats. Conclusion: The pharmacognostic profile
[Downloaded free from https://www.jaim.in on Monday, February 15, 2016, IP: 115.112.118.203]
Abstracts
These abstracts were presented at the Update Ayurveda 2014 at Mumbai, Maharashtra, India. The abstracts were screened
by the scientific committee of Update Ayurveda 2014 and are not peer reviewed by J-AIM. © Update Ayurveda 2014.
14
for M. elengi flowers obtained as a finding of this research
work will contribute in their identification and standardization.
The validated HPTLC methods for estimation of different
bioactive marker can be applied to various other herbal drugs/
formulations for the estimation.
UA/OP/29
Estrogenic effect of an Ayurvedic medicinal plant:
Saraca asoca flowers
Gauri Swar, Sunita Shailajan
Herbal Research Lab, Ramnarain Ruia College, Matunga, Mumbai,
Maharashtra, India
E‑mail: gaurirswar@gmail.com
Background: Saraca asoca (Roxb). De Wilde. (Caesalpiniaceae),
popular as Ashoka, is a medium evergreen tree regarded as
one of the most important indigenous medicinal plants in India,
which has been claimed for its potential use in women’s’
health problems. Flowers are used in the treatment of
diabetes, cancer, and hemorrhagic dysentery and are given
as tonic to females with uterine disorders. Objective: The
study was designed to investigate the estrogenic potential
of standardized ethanolic extract of S. asoca flowers
using ovariectomized female albino Wistar rat. Materials
and Methods: Ethanolic extract of S. Asoca flowers has
been standardized in terms of its kaempferol content using
validated HPTLC method. Safety of the extract was evaluated
at 2000 mg/kg body weight in female albino Wistar rats.
Ovariectomized animals were administered with the extract
at three dose levels ‑ 100 mg/kg, 200 mg/kg and 400 mg/kg
body weight orally once for two weeks. Estrogenic effect was
studied by assaying the activities of various serum and plasma
marker enzymes and hormones in addition to the effect on
vaginal cornification, uterine histology, and uterotonic effect.
Results: The kaempferol content was found to be 2.3 mg/g of
extract. Estrogenic effect of standardized extract of S. Asoca
was found to be in a dose‑dependent manner. Conclusion:
The standardized extract of S. asoca may be used as an
alternative therapy for the treatment of female reproductive
disorders caused due to estrogen deficiency.
UA/OP/30
Evaluation of polyherbal oral formulation for antidiabetic
activity
Vanita V. Gurale, Savita R. Kulkarni, P. K. Khatwani
Department of Pharmacognosy and Phytochemistry, Bombay
College of Pharmacy, Kalina, Santacruz, Mumbai, Maharashtra,
India
E‑mail: vanita7827@gmail.com
Background: The individual extracts of leaves of Murraya
koenigii (MK), Annona squamosa (AS) and roots of Plumbago
zeylanica (PZ) had shown promising results in oral glucose
tolerance test (OGTT) model. Objective: In order to study the
potential synergistic activity of all these herbs, the capsules
of each containing 200 mg extract of each of MK and AS
and 40 mg of powder of PZ were prepared and tested for
antidiabetic activity in STZ induced diabetic rat model.
Materials and Methods: The aqueous extracts of all the
ingredients that is, leaves of MK, AS, and root powder of PZ
were prepared by hot decoction method and extracts were
evaporated to get fine powder. The optimized dose revealed
from OGTT study was selected for the capsule formulation.
All the ingredients of the capsules were mixed together in
required proportion with suitable excipients and filled into the
capsules. The capsules were studied for their physicochemical
stability, content of phytochemical marker by HPTLC and their
antidiabetic activity using STZ induced diabetic rat model.
Results: The OGTT study of individual extracts had revealed
that at the dose of 200 mg for MK and AS, lowered blood
glucose by 64%. The dose of the polyherbal formulation was
calculated by extrapolation of these studies to human dose.
The current study showed that the polyherbal formulation was
64% as effective as glibenclamide in STZ induced diabetic rat
model. All the constituent herbs of capsule contain rutin that
is known to have antidiabetic activity. Conclusion: Hence, a
good oral polyherbal formulation is available for management
of Diabetes.
UA/OP/31
Enhancement of wound healing activity utilizing
combination of napthoquinone dimer from Onosma
echioides and Yashad bhasma
Purnima Vivek, Nikita Gawand, Chhaya Gadgoli
Department of Pharmacognosy, Saraswathi Vidya Bhavan’s College
of Pharmacy, Dombivli, Mumbai, Maharashtra, India
E‑mail: purnimaviv@rediffmail.com
Background: Root bark of Onosma echioides is commonly
utilized in folklore medicines to treat skin eruptions. It is
rich in naphthoquinones, alkanins, and shikonins. Yashad
bhasma is rich in zinc oxide is known as mild astringent,
antiseptic and accelerates wound healing. Objective: The
present study is aimed at preparation of a combination of
isolated naphthoquinone dimer [C40 H44 O10] with Yashad
bhasma and to evaluate its wound healing potential.
Materials and Methods: The isolated compound OE1,
Yashad bhasma and combination of OE1 + Yashad bhasma
were incorporated in simple ointment base in concentration
0.25%w/w, 1.25%w/w and 0.125%w/w + 0.62%w/w
respectively. The above formulations were evaluated
for the wound healing activity in rats using excision and
incision wound models. Determination of parameters
viz. period of epithelization and hydroxyproline content
in Eschar in excision wound model while in incision
wound model the tensile strength of new skin was
determined. Results: The combination of OE1 and Yasahad
bhasma (0.25%w/w + 0.62%w/w) showed to have better
wound healing activity as significant (P < 0.001) reduction
in the epithelization period and significant (P < 0.001)
increase in hydroxyproline content and tensile strength
than the isolated compound (0.25%w/w) and Yashad
bhasma (1.25%w/w). Conclusion: Isolated compound OE1
when used in combination with Yashad bhasma exhibited
better wound healing potential than OE1.
UP/OP/32
Effect of Pratisarniya kshara in the management of
Gudabhramsha
Shikha Nayak, P. Hemantha Kumar
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Abstracts
These abstracts were presented at the Update Ayurveda 2014 at Mumbai, Maharashtra, India. The abstracts were screened
by the scientific committee of Update Ayurveda 2014 and are not peer reviewed by J-AIM. © Update Ayurveda 2014.
15
Department of Shalya Tantra, National Institute of Ayurveda, Jaipur,
Rajasthan, India
E‑mail: shikhanayak24@gmail.com
Background: The disease Gudabhramsha, which is described
in Ayurvedic literature and rectal prolapse are one and the
same disease with different terminology. Sushruta has
described Kshara karma where Pratisaraneeya Kshara Karma
is described in Arsha Chikitsa, in the context of ‘Bhrasta
Guda’ (Rectal Prolapse), which is said to be the effective one.
Objectives: To evaluate the effect of “Pratisaraneeya Kshara
Karma” in the management of Gudabhramsha. Materials
and Methods: Totally, 20 patients were randomly selected
for Apamarga Pratisaraneeya Kshara Karma. During this
treatment, the patients were observed for daily improvement
and relative observations were noted on 1st, 2nd, 3rd, and
7th day. After that, patients were advised to come for
follow‑up once in a week, for 1 month and then once in a
month for 6‑months. All the cases were assessed as per the
clinical criteria fixed for the study that is, degree of prolapse
and anal sphincter tonicity. Results: In the present study, the
improvement of degree of rectal prolapse showed that, on 1st,
2nd, 3rd, and 7th day, it was 91.4% improvement. But at the
end of 1st month, due to recurrence occurred in some cases
the improvement in the degree of Prolapse was decreased
from 91.4% to 87.23%. On 6th month also it was 87.23%.
However, 4th degree rectal prolapse shows poor results in
this study. Conclusion: After collecting all the research data,
clinical profiles, reports, observations and results of this
study, the efficacy of Pratisaraneeya Kshara Karma appears
to be very effective in Gudabhramsha (Rectal Prolapse).
UA/OP/33
Assessment of state of aggravated Doshas through
physical and bio‑chemical examination of blood:
A diagnostic study
Mukesh Bhayal, Naresh Kumar Mahawar, Reetu Sharma,
Sisir Kumar Mandal, Surendra Kumar Sharma
Department of Roga Nidana Evam Vikriti Vijnana, National Institute
of Ayurveda, Jaipur, Rajasthan, India
E‑mail: mukeshbhayal@gmail.com
Background: Ayurveda is the clinical science of medicines
that has holistic approach to improve the quality of life
of an individual. The theory of tridosha is the foundation
of Ayurveda. Doshas are not stable, they decrease,
increase or remain same at every moment and they
govern the physiological and physiochemical activities.
Objective: To establish the correlation between the
states of aggravated doshas and laboratory investigation.
Materials and Methods: Total 99 subjects were selected
randomly from OPD and IPD of our institute. The patients
were examined clinically through questionnaires, and
their physical examinations were recorded in a performa.
Results: Doshik imbalance is the initial cause of any disease.
Doshik imbalance reflects on physical, mental as well as
bio‑chemical character of the body. Low‑density lipoprotein,
triglyceride, very‑low‑density lipoprotein indicates aggravated
kapha involved in diseases like prameha, medoroga, kaphaja
hridroga etc., Blood urea level was slightly higher in pitta and
vata prakriti indicating pitta is usually related to increased
metabolic effect in the body. Conclusion: There are no
objective parameters to assess the doshik state in the body,
we usually assess doshik vriddhi and kshaya state based
on clinical features.It was concluded that there are some
variations in the bio‑chemical factors that may help to assess
the condition of doshas very easily and accurately.
UA/OP/34
Multiple lipomatosis and its Ayurvedic management
Pranav P. Redkar, Yunus G. Solanki
Department of Shalya Tantra, Government Ayurved College,
Osmanabad, Maharashtra, India
E‑mail: drpranav213@gmail.com
Background: Lipomatosis is a rare disorder of unknown
etiology in which multiple lipomas are present on the body.
Many discrete, encapsulated lipomas are formed on the trunk
and extremities, with relatively few appearing on head and
shoulders and not usually seen on face, scalp, plantar and
palmar surface. Lipomas are usually observed between third to
fifth decades of life. Existing Lipomas keep on growing in size
and newer lipomas keep on appearing, disfiguring the body
parts. In Ayurveda this condition is known as Medoj granthi
and it is produced as a result of vitiation of Meda dhatu. There
is no known remedy for multiple lipomatosis in contemporary
that is, modern medicine except surgical removal that is
inadequate. Objective: To assess the effect of Ayurvedic
remedy in the management of multiple lipomatosis. Materials
and Methods: In the present study, seven patients of multiple
lipomatosis were treated with Ayurvedic medicine. Detail
family history was taken, and family members assessed for
multiple lipomatosis. Patients were thoroughly investigated.
According to prakriti and rutu, vidhipurvak shodhan chikitsa
followed by Shaman Chikitsa comprising of trikatu churna,
kanchanar guggul and medohar gana kashaya of Charaka
samhita was given for 6 months. Follow up was taken after
every 15 days for 3 months and thereafter every month for
next 3 months. Result: As a result of above treatment, the
growth of existing lipoma and appearance of new lipomas
was arrested. Conclusion: Ayurvedic formulations followed
by Shodhan chikitsa is effective in multiple lipomatosis and
should be persuade for a longer time.
UA/OP/35
Role of Kushthadi lepa and Guduchi churna in the
management of Vicharchika
Saroj Choudhary, Chetana Agrawal, Sisir Kumar Mandal,
Surendra Kumar Sharma, S. K. Khandal
Department of Roga Nidan and Vikriti Vijnana, National Institute of
Ayurveda, Jaipur, Rajasthan, India
E‑mail: sarojchaudhary@gmail.com
Background: Vicharchika is described under Kshudra kushtha
in Ayurvedic classics, also mentioned as a curable disease
yet the relapsing nature of this disease makes it much
harassment for patient and also troublesome for physician. The
disease Vicharchika is not a life‑threatening one, but makes
patient upset due to its appearance. Objective: To find out
the role of Kushthadi Lepa and Guduchi Churna (Tinospora
cordifolia Thunb. Miers) in the management of Vicharchika.
[Downloaded free from https://www.jaim.in on Monday, February 15, 2016, IP: 115.112.118.203]
Abstracts
These abstracts were presented at the Update Ayurveda 2014 at Mumbai, Maharashtra, India. The abstracts were screened
by the scientific committee of Update Ayurveda 2014 and are not peer reviewed by J-AIM. © Update Ayurveda 2014.
16
Materials and Methods: For this study, total 30 patients of
age group 18 to 70 years were selected and were equally
categorized into three groups. Group A patients were given Lepa
Q. S and Guduchi Churna 5 g twice a day after Samsodhana
karma (Virechana karma). Group B patients were advised
for Lepa along with Guduchi churna. Group C patients were
advised for Lepa only. The duration of the trial was 30 days.
Result: Highly significant relief was seen in all the parameters
of Groups A, B and C respectively that is, marked improvement
in erythema (60%, 47.6%, 55%), Pidika (68.4%, 58%, 54%),
pruritus (56.52%, 59%, 62%), oozing (66%, 83%, 62%).
Although the relief was seen, it was not statistically significant.
Conclusion: Virechana Karma provides comparatively better
results in symptoms of Vicharchika like Shotha, Svayathu,
Kandu and Pidika. The trial drug Guduchi acted as an
immunomodulator to combat the disease.
UA/OP/36
Formulation development of an anti‑inflammatory
herbal transdermal film
Chintan Bhavsar, Trusha Parekh, Anjali Gohel, Tabassum Khan
SVKM’s Dr. Bhanuben Nanavati College of Pharmacy, Mumbai, India
Email: chintanbhavsar93@gmail.com
Background: The conventional oral anti‑inflammatory drugs
available today are associated with side effects like gastric
irritation thereby reducing the patient compliance for chronic
use. Herbal medicines are believed to be relatively safe and
effective. Hence, an anti‑inflammatory herbal transdermal
film was formulated. Objective: To formulate an herbal
transdermal film containing anti‑inflammatory extract.
Materials and methods: The Methanol extract of Acacia
nilotica (F: Mimocaceae) pods (MEA) exhibited a significant
in vivo anti‑inflammatory activity in rats and it also showed
a significant anti‑arthritic activity in our laboratory studies.
Also, Cinnamon oil (Cinnamon zeylanicum) and Lemon
oil (Citrus limon) were incorporated into the formulation
for synergistic activity. 1g each of lemon oil and cinnamon
oil was emulsified in glycerin using Tween80 as emulsifier.
Polyvinyl pyrrolidone and polyvinyl alcohol were dissolved
in adequate hot distilled water. 10% MEA in DMSO and the
emulsion prepared was added to the above aqueous solution
and uniformly stirred. This solution was poured in petri
plates and allowed to dry to form the film. Physicochemical
evaluation tests and preliminary diffusion studies were
carried out using Franz diffusion cell and the results were
obtained. Results: The film obtained was uniform in terms
of weight, flatness, percent moisture loss and thickness.
Preliminary diffusion studies that were carried out showed
satisfactory drug release through the Nylon 6.6‑ 0.45μ lipid
membrane. Conclusion: The herbal transdermal film was
prepared successfully. This film can be used as an alternative
to the oral NSAIDs for chronic treatment of arthritis.
UA/OP/37
A randomized controlled trial of Apamarga (Achyranthus
aspera L.) and Vyoshadi Guggulu on Sthaulya
(Overweight)
Manisha Talekar1, Pawankumar Godatwar1,
Sisir Kumar Mandal1, Sudhir Turi2
1Department of Roga and Vikriti Vijnana, National Institute of
Ayurveda, Jaipur, Rajasthan, 2SSMB Ayu College, Moga, India
Email: dr.mani21jan@gmail.com
Background: Obesity is one of the highly neglected
health problems and a type of which invites many major
and minor diseases. It certainly shortens the life‑span.
Presently obesity has turned in to a pandemic and needs
attention from all aspects of its control. Objective: The
Present study was done to assess the efficacy of trial drug
Apamarga Tandula and Vyoshadi Guggulu in comparison to
standard drug Sibutramine on Anthropometric Parameters.
Materials and Methods: 60 subjects were randomly
divided in three groups. Apamarga Tandula was given
to group A, Apamarga Tandula and Vyoshadi Guggulu
was given to group B and the standard drug Sibutramine
was given to group C. The subjects were assessed every
15 days‑ before start, once during and at the end of trial of
8 weeks. Results: Apamarga Tandula showed significant
improvement in body fat %, Waist circumference and SKFT
while Apamarga Tandula and Vyoshadi Guggulu showed
significant improvement in BMR, Waist circumference and
SKFT. The control drug sibutramine showed significant
improvement in body fat % and Waist circumference.
Conclusion: When the adverse affects of the control
drug sibutramine are taken into account, it can be safely
concluded that the Apamarga Tandula is a better drug for
the management of obesity especially in combination with
Vyoshadi guggulu than the control drug sibutramine.
UA/OP/38
A clinical study of changes in the lipid profile after
administration of Amrutadhya Guggulu w.s.r. to
hyperlipidemia
Barapatre Pranali S, Wankhade VH
Department of Kaychikitsa, Vidarbha Ayurved Mahavidyalaya,
Amravati, India
Email: pbarapatre317@gmail.com
Background: Hyperlipidemia is one of the major lifestyle
disorders. It has been identified as a potential risk factor in
the manifestation of serious diseases like Ischemic Heart
Disease (IHD), Diabetes, Stroke etc., which is an important
cause of morbidity and mortality all over the world. These
lifestyle disorders have been referred to as the Santarpanjanya
Vyadhis in the classical texts. As Guggulu is most potent
drug in Hyperlipidemia also proven its Karshan property,
Guggulu compound i. e. Amrutadhya Guggulu as indicated
in Sthoulya Chikitsa by Aacharya Chakradatta was selected
for the clinical study. Objectives: To evaluate the clinical
efficacy of Amrutadhya guggulu and to study the changes
in lipid profile after administration of Amrutadhya guggulu in
Hyperlipidemia. Material and Methods: 60 clinically diagnosed
patients attending the OPD/IPD of our institute were selected
for the present study randomly and divided in two groups.
Group A contained 30 patients to which Amrutadhya Guggulu
500mg TDS was given and Group B contained 30 patients
which were on placebo. Duration of treatment was 60 days;
follow‑up was taken every 15 days. Result: The result of the
study revealed that Amrutadhya guggulu was found to have a
significant effect in the lipid profile. Conclusion: Amrutadhya
guggulu proved effective in treating Hyperlipidemia.
[Downloaded free from https://www.jaim.in on Monday, February 15, 2016, IP: 115.112.118.203]
Abstracts
These abstracts were presented at the Update Ayurveda 2014 at Mumbai, Maharashtra, India. The abstracts were screened
by the scientific committee of Update Ayurveda 2014 and are not peer reviewed by J-AIM. © Update Ayurveda 2014.
17
UA/OP/39
Virechana karma‑A statistical analysis
Rahul Singh Sisodia, B.S.Prasad
KLE University’s Shri BMK Ayurveda Mahavidyalaya, Belagaum, India
Email: dr.rahulsingh0508@gmail.com
Background: Panchakarma is a set of five procedures
claimed to eliminate the vitiated doshas from the body
and Virechana is one of them. For Virechana karma
kostha assessment is necessary and Virechana karma is
administered with different Virechaka yogas such as Trivrita
Lehya, Dantyavaleha, Abhayadi Modaka etc. Objective: To
assess the efficacy of Trivrita Lehya on types of kosthas.
Materials and Methods: In KLE Ayurveda Hospital, Belgaum
total number of virechana administered from January
2012 to September 2014 is 753. All Virechana karmas
were selected to carry out retrospective study irrespective
of disease. Out of them 409 patients (54.31%) were
having krura kostha, 126 (16.73%) madhyama kostha
and 218 patients (28.95%) Mridu Kostha. Out of them
621 Patients (82.47%) were administered with Trivrita
lehya and rest 132 patients (17.52%) administered with
other Virechaka Yogas. In krura kosthi patients 30 gm
Trivrita Leha is given to 291 (71.14%), in Madhyama
kosthi 92 patients (73.01%) 25 gm Trivrita Leha given, in
Mridu Koshthi 189 (86.69%) 20 gm of Trivrita Leha given.
Results: By Trivrita lehya madhyama, avara, pravara shuddhi
observed in krura, madhyama, mridu kostha respectively.
Conclusion: If kostha assessment and dose calculation is
perfect then Trivrita leha can give good results in Virechana.
UA/OP/40
Handshake of Ayurveda with modern science: Novel
approach towards development of antimicrobial skin
formulations
Divij Kotian, Rujuta Deshpande, Shubha Govil,
Aparna Deshmukh
Thakur College of Science and Commerce, Mumbai, India
Email: drasdeshmukh@gmail.com
Background: Modern antimicrobial formulations are focused
to act on microbes, and not the hosts. Ayurveda prescribes
herbal formulations indicated for multiple skin conditions and
also for improving host immune mechanisms. Benefits of
nanoparticles in antimicrobial formations are already reported.
This prompted us to explore in vitro the novel approach of
combining modern scientific tools with ancient wisdom of
Ayurveda as a step towards development of antimicrobial
formulations. Objective: The study aimed to evaluate in vitro
anti‑microbial and immuno‑modulatory activity of various
herbs indicated in various skin infections, ZnO nanoparticles
and their combinations. Materials and Methods: 23 herbal
preparations of herbs, ZnO nanoparticles and their
combinations were tested for antimicrobial effect against
pathological isolates of Staphylococcus aureus and Candida
albicans using agar cup method. Their effects on the first line
of host defense were studied by determination of neutrophil
phagocytic activity. Results: Rubia cordifolia (Manjishtha),
Allium sativum (Garlic), Piper betle (Beetle), Ocimum
sanctum (Tulsi), Terminalia chebula (Harade), Azadirachta
indica (Neem) and Ficus bengalensis (vat) were effective
against Staphylococcus aureus and Candida albicans.
Maximum stimulation of neutrophil phagocytosis was
observed with Rubia cordifolia (% phagocytosis 86.6 vs.
control 37 ± 10 and phagocytic index 1.66 vs. control
1.3 ± 0.2) and Ocimum sanctum (phagocytic index 1.95
and % phagocytosis 65.4). ZnO nanoparticles in the range
of 0.1‑ 0.5 mg/ml showed antimicrobial activity and also
stimulation of phagocytosis (% phagocytosis 87.5; PI: 1.69).
Conclusion: Amongst the tested combinations, additive but
not synergistic antimicrobial action was observed between
ZnO nanoparticles and Manjishtha decoction.
UA/OP/41
Development and physico‑chemical evaluation of pain
balm ointment by Gunja seeds
Prashant Kumar Singh, SantoshS. B, K.S. Gudaganatti,
R.R.Hiremath
KLEUs BMK Ayurveda Mahavidyalaya Shahapur Belgaum, India
Email: singh9947@yahoo.com
Background: Gunja (Abrusprecatous) is an herbal poisonous
drug mentioned under the group of upavishas in Ayurveda.
External application of paste of Gunja seeds have said to be
useful in the management of pain. Objective: To develop an
ointment by using extract of Gunja seeds and other bases by
fusion method. Materials and Methods: Soxhlet extraction
of Gunja seeds was prepared by using ethyl alcohol. The
ointment base was prepared using fusion method by using
hard paraffin 370 C 5%, Cetostearyl alcohol 48‑560 C 5%,
Lanolin 370 C 8%, Bees wax 62‑640 C 10%, Soft Paraffin
370 C 72%. The ratio of base and extract was base 90% and
extract of Gunja seeds 10%. Soon after cooling the ointment
was stored in air tight container. Results: Physicochemical
results of Gunja seeds showed foreign matter 1%, Total
ash 2.75%w/w, Acid insoluble ash 0.37%w/w, Water
soluble ash 0.74%w/w, Alcohol soluble extracts 12.8%,
Water soluble extracts 19.6%, pH 6.15. HPTLC Analysis of
ethanolic extract of Gunja showed 5 bands at 245nm with
Rf values viz. 0.06, 0.19, 0.24, 0.29, 0.44. And showed
7 bands at 366nm with Rf values viz. 0.03, 0.07, 0.35,
0.42, 0.48, 0.56, 0.67. The analytical results of the Gunja
Ointment is pH 5.9 2, Spread ability 86.8 Sec/G/Cm, MLT
Negative, Viscosity is 5120mPas. Conclusion: Prepared
ointment showed agreeable organoleptic characteristics and
it passes all the analytical tests as conducted for semisolid
dosage forms, along with it showed good stability for the
period of 8 months at room temperature.
UA/OP/42
Treadmill stress test as a measure of Deha bala w.s.r.
to Pandu roga
Ashish Reja1, Pawankumar Godatwar1, Vinod Chandra Singh1,
Sevatkar Balkrishan1, Sisir Kumar Mandal2
1Department of Roga nidana evam Vikriti vijnana, N.I.A. Jaipur,
Rajasthan, 2Durga Ayurveda College, Raipur, Rajasthan, India
Email: dr.ashishreja@gmail.com
Background: Deha‑bala implies physical capability
of an individual. The characteristics of deha‑bala are
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Abstracts
These abstracts were presented at the Update Ayurveda 2014 at Mumbai, Maharashtra, India. The abstracts were screened
by the scientific committee of Update Ayurveda 2014 and are not peer reviewed by J-AIM. © Update Ayurveda 2014.
18
Utsahalakshana when one can perform work in unfavorable
conditions also and Upachayalakshana can be understood
by body built up, stability and compactness of the body.
Objective: The Present study was done to evaluate Treadmill
Stress Test as a measure of Deha‑bala in Pandu roga.
Materials and Methods: 60 subjects of Pandu roga were
recruited for the study after written informed consent and
randomly divided in two groups. Balya churna was given
to group A and Nisha loha vati was given to group B.
Deha‑bala was measured with help of Treadmill Stress Test
which was Bruce protocol (Dr. Robert. A. Bruce, 1963).
Results: A significant increase in deha‑bala was found in
group A from (2.10 to 3.30), while in group B it increased
from (2.07 to 3.23). Conclusion: It can be concluded
that Treadmill Stress Test was an efficient objective for
measuring the Deha‑bala status.
UA/OP/43
Evaluation and standardization of Arogyavardhini Vati
Rohini Waghamare, Pratima Tatke
Department of Pharmaceutical Chemistry, C.U. Shah College of
Pharmacy, S.N.D.T. Women’s University, Santacruz, Maharashtra,
India
Email: rohini.chandratre@gmail.com
Background: Arogyavardhini Vati is an ancient well
known Ayurvedic formulation. It is widely used in the
treatment in the skin disorders, fever and problems
related to digestive system. The Vati consists of different
crude drugs such as Kutaki, Haritaki, Bibhtaka, Amalaki,
Eranda, Guggulu, Neemleaves, Shilajatushudhhaand
Neemjuice and the metal and metal preparations such
as purified mercury, purified sulfur, lauhabhasma,
abhrakbhasma, tamrabhasma. However the systematic
evaluation of this formulation is yet not reported
and published which is essential for its world‑wide
acceptance. Objective: To evaluate and standardize
Arogyavardhini Vati as per Ayurvedic Pharmacopoeia
of India. Methodology: Macroscopic and microscopic
evaluation of the Arogyavardhini Vati is carried out by
pharmacopoeial methods. The other parameters such
as, weight variation, size, hardness, disintegration time,
friability, heavy metal analysisare also evaluated and
standardized as per Ayurvedic Pharmacopoeia of India.
HPLC profiles/HPTLC finger prints have been developed.
Results: Microscopic evaluation confirmed the presence
of the crude drugs ingredients. The HPLC profile/HPTLC
finger prints reports the marker compound from the
crude drug in the formula. The other pharmacopoeial
parameters are within the limits standards specified in
Ayurvedic Pharmacopoeia of India. Conclusion: The study
evaluates and compares Arogyavardhini Vati of two
different manufacturers. This study will set the guidelines
for quality control of Arogyavardhini Vati.
UA/OP/44
Comparative in vitro Anthelmintic activity of two
ayurvedic herbal drugs
Kulkarni Aniruddha Shreedhar1, Kulkarni Anuja Aniruddha2,
Khot Vidyarani Sujitkumar3
1Vaidyaraj Datar Panchabhautik Chikitsa and Samshodhan Kendra,
Sangli, 2Department of Hospital and clinical pharmacy, Dr. J. J. Magdum
Pharmacy College, Jaysingpur, 3Department of Pharmacology and
Toxicology, Dr. J. J. Magdum Pharmacy College, Jaysingpur, India
Email: askulkarni2@gmail.com
Background: The World Health Organization (WHO) estimates
that infection with round worm, whipworm and hookworms
with associated morbidity, affect approximately 250 million,
46 million and 151 million people, respectively. So, worm
infestation as a public health problem needs immediate
attention. Sadly, very few drugs are used for deworming
specially in children. Objective: The main objective of
the present study was to compare in vitro anthelmintic
activity of two herbal ayurvedic drugs namely Kapila and
Chaturthak yoga, used as vermicides along with Albendazole
as a standard reference. Materials and Methods: The two
Ayurvedic herbal drugs presented in this study are Kapila
and Chaturthaka yoga. The aqueous and methanolic extracts
of these drugs were prepared using maceration, soxhlet
apparatus and thermostatic bath. These extracts were
investigated for anthelmintic activity using earthworms
(Pherentima posthuma). Albendazole was used as a reference
standard at 10 mg/ml. Results: Aqueous and methanolic
extracts of these two drugs at different concentrations
(10, 20 and 40mg/ml) demonstrated dose dependent
vermicidal activities. Aqueous extract of Kapila at 40 mg/ml
caused paralysis and death at 18.83 and 36.5 minutes;
while it’s methanolic extract showed paralysis and death at
21.33 and 46.66 minutes respectively. Aqueous extract of
Chaturthaka yoga at 40 mg/ml caused paralysis and death at
10 and 18.83 minutes; while it’s methanolic extract showed
paralysis and death at 6.16 and 19.33 minutes respectively.
Conclusion: Both the drugs possess potent anthelmintic
activity when compared to Albendazole. Chaturthaka yoga
is the most effective drug.
UA/OP/45
Anti secretary and anti ulcer activity of Yashtimadhu
Ghrita and Pittantak Yog‑ An experimental study
Varsha Mundhra, Jagadeesh G. Mitti, K. Shankar Rao
National Institute of Ayurveda, Jaipur, India
Email: dr.varshamundhra@gmail.com
Background: The science of Ayurveda, directs towards
the different secretions from gastrum and liver as
pitta. Abnormal secretions may leads to acid peptic
disorders (APD) which can be correlated with diseases
viz., Amlapitta, Annadrava shool and Parinaam shool.
These may lead to hematemesis, melena, and perforation
if failed to treat them in time. Objective: To evaluate the
gastric anti secretary and anti ulcer activity of Yastimadhu
Ghrita and to find out the effective formulation among
the two selected formulations. Materials and Methods:
Yashtimadhu ghrita (anubhoot yog) and Pittantak Yog (NIA
pharmacopeia) were taken for the study. Yashtimadhu
ghrita and Pittantak yog were compared experimentally
with Ranitidine (standard drug) for anti‑secretory and
anti‑ulcer activity in albino rats weighing 150‑200 gm.
in four groups (six rats in each). Results: The ulcer index
in Control, Standard, Piitantaka yoga and Yastimadhu
ghrita group was 6.3, 0.85, 0.9 ± 0.2, 1.3 ± 0.28 and
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Abstracts
These abstracts were presented at the Update Ayurveda 2014 at Mumbai, Maharashtra, India. The abstracts were screened
by the scientific committee of Update Ayurveda 2014 and are not peer reviewed by J-AIM. © Update Ayurveda 2014.
19
1.5 ± 0.22. The results were statistically significant by‘t’
test. Conclusion: The results found were significant and
equipotent with each other.
UA/OP/46
Concept of Oja vis‑a‑vis endoplasmic reticular
function: an approach to decode the holistic view
Anagha Ranade, Rabinarayan Acharya
Department of Dravyaguna, IPGT and RA, G.A.U, Jamnagar, India
Email: anagharanade11@gmail.com
Background: In Ayurveda, Oja, being one of the most
distinctive concepts is a factor of prime importance related to
vitality. Ayurveda depicts the definition, types (Para, Apara);
site (Hridaya‑ Heart, sarvashariravyapi – presence in cellular
level), quantity (vindu ‑ in drops), function (bala‑vitality) and
its derangement etiology etc., of Oja.Various researchers
based on their own ideas have given its modern parlance
with immunity and stem cell etc. Objective: To decipher
the fundamental concept of Oja vis‑à‑vis endoplasmic
reticular function. Material and Methods: A critical
analysis of information on Oja from available Ayurvedic
literature and endoplasmic reticular function form modern
Physiology texts. Result: ‘Endoplasmic reticulum (ER)’
is a multifunctional membranous organelle forming a
network in the whole body and is responsible for lipid
biosynthesis, calcium storage and protein folding and
processing. Etiological factors of Ojadushti (dysfunction)
are (Abhighata‑may be environmental stress), (shoka‑mental
stress), (shrama‑physical stress, etc) which share similarity
with stressors that disrupt function of ER ultimately
leading to ER stress causing lifestyle disorders. Achieving
cellular homeostasis is now being emphasized in the
management of various diseases, in conventional medicine.
The cellular homeostasis is dependent largely on proper
folding of proteins where as in proper folding results into
accumulation of misfolded proteins ultimately enhancing
cellular death. Scientific reports on vayathapana herbs
like Amalaki, Punarnava, Guduchi etc., to alleviate ER
stress can be give an evidence to correlate the ER with
Oja. Conclusion: The present hypothesis gives an insight
to decipher a novel concept of Oja and its relation with
Endoplasmic reticulum.
UA/OP/47
Pharmacognostical evaluation of Ipomoea pentaphylla
Linn
Minutee Patel, Rajesh Mer, Preeti Pandiya, Anagha Ranade,
RN Acharya
Department of Dravyguna, IPGT and RA, GAU, Jamnagar, India
E-mail: drrnacharya@gmail.com
Background: In the present era, the genuine identification
of crude drug is posing a problem to the physicians and
pharmaceuticals. Medicinal plants used in Ayurveda
are often found to be adulterated and substituted
which decreases the efficacy of the drugs in practice.
Pharmacognosy is a prime tool of basic authentication of
medicinal plants in order to standardize them. Ipomoea
pentaphylla Linn, a tribal claimed medicinal plant, not
scientifically evaluated and reported till yet, was assessed
for its basic pharmacognostical characters. Objective: To
evaluate pharmacognostical parameters of whole plant of
I. pentaphylla. Material and Methods: The fresh plant was
collected, identified, authenticated and assessed for its
macroscopy followed by microscopy of leaf, petiole and
seeds. The powder microscopy, histo‑chemical study and
stomatal index were also studied following the standard
procedures given in authentic text and the measurements
of specific characters were noted and microphotographs
were recorded. Results: The morphological characters of
I. pentaphylla strongly support the Convolvulaceae family.
The microscopic characters of root, stem, leaf, petiole, and
seed showed presence of cortex, epidermis, parenchymatous
tissue and vascular bundle along with presence of cluster,
rosette and prismatic crystals, trichomes, starch grains, etc.
The seed mainly showed presence of mucilage containing
gland. The stomatal index was 20 (upper surface) and
20 (lower surface). Powder microscopy revealed presence
of simple and compound starch grains, cluster and rosette
crystals of calcium oxalate, oil globules, and paracytic
stomata. Conclusion: The present pharmacognostical
observations may be considered as a standard parameter
for basic identification of I. pentaphylla linn.
UA/OP/48
Clinical Study on the effect of Bringaraj Punarnava
Ghana Vati on Uccharaktachapa with special reference
to essential hypertension
Anupama Mahadeorao Bathe, Ajay Kumar Sharma,
Ajay Kumar Sahu
Department of Kayachikitsa, National Institute of Ayurveda, Jaipur,
Rajasthan, India
Email: dranupamabathe@gmail.com
Background: Hypertension is asymptomatic in nature but
produces dreadful effects on the body. So it is called
as silent or hidden killer. Due to its high prevalence
Indian Council of Medical Research (ICMR) and All India
Institute of Medical Sciences (AIIMS) have declared
India as a “Nation of Hypertension”. Whole world is
looking for the Ayurvedic management of the same.
Objective: To study the efficacy of Bhringaraj Punarnava
Ghanvati in Uccharaktachapa (Essential‑hypertension).
Material and Method: 15 patients were selected randomly
from Arogyashala OPD and IPD, of our institute, which were
well diagnosed of Uccharaktachapa/Essential Hypertension
I and II grade as per 7th JNC and WHO criteria for Diagnosis
Hypertension and symptoms described in Ayurvedic classical
text. Bringraj‑Punarnava ghana vati was given in dose of
2 tab. (each 500 mg.) three times in a day with lukewarm
water for 45 days and IPD subjects were assessed routinely
while OPD in 7 days follow up. Results: Bringraj‑Punarnava
ghana vati showed highly significant result in both the
systolic and diastolic blood pressure and significant
improvement in subjective parameters shirashool, bhrama,
klama, hritspandan and anidra. Conclusion: Therapy in the
form of administration of Bhringraj Punarnava ghan vati is
safe and effective in the management of Uccharaktachapa
(Essential Hypertension).
[Downloaded free from https://www.jaim.in on Monday, February 15, 2016, IP: 115.112.118.203]
Abstracts
These abstracts were presented at the Update Ayurveda 2014 at Mumbai, Maharashtra, India. The abstracts were screened
by the scientific committee of Update Ayurveda 2014 and are not peer reviewed by J-AIM. © Update Ayurveda 2014.
20
UA/OP/49
Can In Silico Study Shift The Paradigms In Ayurveda
Drug Research Leading To Prakruti Based Medication?
Harshad Satish Rasane, Ravindra P. Bhurke
Department of Kriya Sharir, R.A. Podar Medical College (Ayu.),
Worli, Mumbai, Maharashtra, India
Email: drrasane@gmail.com
Ayurveda advices the use of various medicinal herbs in
treatment as well as in day to day activity. This conceptual
application directly or indirectly tries to maintain the body’s
equilibrium. The proper knowledge of such equilibrium
is best available under the concept of Prakruti. The
genetic and the genomic study can be correlated with the
concept of Prakruti. The boom in the field of genome
has led to existence of huge magnitude of data which is
better maintained, utilized and applied with the help of
bioinformatics. The in‑depth knowledge of Prakruti and
in silico study with simultaneous aid of bioinformatics can
be beneficial and lead to development of Prakruti based
medicine.
UA/OP/50
Validation of Retopareeksha vis‑à‑vis semen analysis
Sukumar Nandigoudar, Anju Sharma1
Department of Kayachikitsa, KLEU’s Shri BMK Ayurved Mahavidyalaya,
PG Studies and Research Centre, Belgaum, Karnataka, 1Department of
Svasthavritta, National Institute of Ayurveda, Jaipur, Rajasthan, India
E-mail: dr.annu1802@gmail.com
Background: Śukra is considered as supreme dhatu
and its dushti leads to male infertility and sexual
dysfunction. Retopareeksha is a source to understand
various Śukra dushti. Semen analysis is one of the important
diagnostic tools in the management of male infertility.
Objectives: To understand and validate retopareeksha as
a standard Ayurvedic diagnostic tools in male infertility.
Materials and Methods: Charaka and sushruta samhita
are the literary resource to assess Shudha śukra (healthy)
and śukra dushti (morbid semen). The qualities of shudha
and dushta śukra will be understood and assessed with
the help of modern seminal parameters. To assess the
parameters of Retopareeksha modern instruments like
phase contrast microscope, Rohem’s sperm counting
chamber, pH measuring strips, calibrated pipette etc., are
used and eosin stains to examine the viability of sperms.
Results: Abnormal physical characteristics of the semen
can be understood by the doshic involvement. Phenila,
Tanu, Ruksa represents vataja śukradushti; Vivarna (neela/
peeta/ashveta) indicate pittaja śukradushti; Atyarta picchila
denotes Kapha dosha; Putipuya indicate pittakapha
involvement and anyadhatu samsrishti is due to shonitadi
dhatu. Conclusion: Retopareeksha focus on panchendriya
pareeksha to assess physical characters and morbid dosha of
semen. Phalavattata, a main character may be attributed to
viability, motility, count and normal morphology of sperms.
The sperm endowed with normalcy of all these is able to
fertilize the ovum that is, Garbhakara. Retopareeksha with
the aid of modern tools gives exact idea about the pathology
involved and thus helps in effective management.
UA/OP/51
An Ayurvedic approach in the management of
neurocysticercosis: A case study
Suraj Rathod, Amit Nakanekar, Sunanda Bhopale,
Ajit Devikar, J. D. Gulhane
Government Ayurved College, Nagpur, Maharashtra, India
E‑mail: suraj_rathod78@yahoo.com
Background: Neurocysticercosis is infection of the brain
by the larval form of the pork tapeworm taenia solium.
Neurocysticercosis is form of cysticercosis and is the most
common infection of central nervous system. In 80% cases
symptoms are seizures, headache, visual disturbance, nausea,
abdominal cramps and diarrhea. Among the rare incidences
some abnormal symptoms occur such as dysarthria,
extraocular movement palsy, hemiplegia, movement
disorders, and gait disturbances. Objective: To see the effect
of Krimi treatment in a case of neurocysticercosis. Materials
and Methods: A 49‑year‑old male patient came to out‑patient
department of our institute with complaints of weakness in
left side of upper and lower limb, slurred speech, difficulty
in walking since 10 days and having H/O seizures 30 years
ago. Patient was diagnosed as a case of neurocysticercosis
through computed tomography (CT) brain, cerebrospinal
fluid, and other hematological investigations. Patient was
admitted and treated with Ayurvedic principle of krimi
management. Results: Patient showed complete recovery in
clinical assessments in terms of speech, gait weakness and
CT scan. Conclusion: Ayurvedic krimi treatment is effective
in the management of neurocysticercosis. However, larger
studies are required.
UA/OP/52
A novel dosage form for enhancement of bioavailability
of herbal topical formulations
Sonali R. Mali, Jyotsna M. Taklikar, Rupali A. Mali1
Department of Rasashastra and Bhaishajya Kalpana,
Loknete Rajarambapu Patil Ayurvedic Medical College,
Post Graduation and Research Center, Islampur, 1Department of
Pharmaceutical Chemistry, Pravara Rural College of Pharmacy,
Pravaranagar, Maharashtra, India
E‑mail: dr.sonalimali@gmail.com.
Ayurveda is a life science, which explains about the
different dosage forms. Topical delivery systems for drugs
make localized administration of the drug anywhere in the
body through ophthalmic, vaginal, skin and rectal routes.
Herbal medicine has become an item of global importance
both medicinal and economical. Although the usage of
these herbal medicines has increased, their quality, safety
and efficiency are serious concerns in industrialized and
developing countries. Hence, turning to safe, effective and
time tested Ayurvedic herbal drug formulation would be a
preferable option for the better patient acceptance. Recently,
pharmaceutical scientists have shifted their focus to
designing a drug delivery system for herbal medicines using
a scientific approach. For a long‑time herbal medicines were
not considered for development as novel formulations owing
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Abstracts
These abstracts were presented at the Update Ayurveda 2014 at Mumbai, Maharashtra, India. The abstracts were screened
by the scientific committee of Update Ayurveda 2014 and are not peer reviewed by J-AIM. © Update Ayurveda 2014.
21
to lack of scientific justification and processing difficulties in
complex polyherbal systems. Novel drug delivery system in
the field of medicine had taken a popular attention as it makes
the intake, bioavaibility and overall therapeutics of drug easier
and in a short period of time. An added complication is that
both the traditional and modern methods of preparation of
herbs are sometimes selected on the basis of technologically
feasible, convenient, or affordable, rather than might be
ideal from the consideration of traditional practices or
clinical efficacy. Considering these facts that review is an
attempt to present a concise profile of different traditional
topical herbal formulations includes lepa, malahara, upanaha,
creams, ointments, pastes and novel topical formulations like
oragnogel, emulgel, ampiphilic gel and transdermal patch.
UA/OP/53
Epidemiological survey of Pranvaha srotodusti w.s.r.
to Vayudusti (air pollution)
Vidya Kumari Meena, Birendra Kumar1,
Surendra Kumar Sharma, Piyush S. Mehta
Department of Roga and Vikriti Vijnana, National Institute
of Ayurveda, Jaipur, Rajasthan.
E‑mail: drmeenavidya@gmail.com
Background: In the present era, due to modern civilization,
urbanization, and industrialization anthropogenic sources are
becoming most responsible for the air pollution. Transport,
power plants, oil refining, burning crop waste, fumes from
paints varnish, aerosol sprays, nuclear weapons, toxic
gases, etc., are main anthropogenic sources responsible for
air pollution. Objective: This study was done to find out the
prevalence of pranvaha sroto dusti in general population of
Jaipur. Materials and Methods: For epidemiological study,
total 500 subjects were surveyed from 5 different places of
Jaipur by camping. Total five groups were made (100 subjects
per group). They were assessed on the basis of subjective
parameters lakshans of pranvaha sroto dusti (swasa,
kasa, hikka and pratishyay) and also on the basis of peak
expiratory flow rate recorded by peak flow meter as objective
parameters. Results: This study showed that prevalence
of pranvaha sroto dusti was more in overweight persons,
short stature persons and smokers. Swas and kasa are the
main symptoms, which persists for long‑time in those who
were exposed for a longer period in that particular polluted
atmosphere. Conclusions: Based on this study, it may be
concluded that pranvaha srotasa is directly connected with
the ambient air (pran vayu) so as the air becomes polluted,
the pranvaha srotasa also becomes vitiate and different kinds
of diseases begin to start in that particular srota.
UA/OP/54
Physicochemical study of different methods of
Gandhaka Shodhana and evaluation of its antimicrobial
property
Shraddha R. Waghmare, Shymal Nirmal
Department of Rasashastra, Ashwi Rural Ayurved College,
Ahmednagar, Maharashtra, India
E‑mail: shraddhawaghmare1989@gmail.com
Background: Gandhak is one of the uprasa mentioned
in rasashasatra. It has been given prime importance in
rasashasatra, because of its rasayana properties. It is also
mentioned in Ayurvedic texts that gandhaka is krumighna.
This property is considered the main section of the desired
study. Micro‑organisms selected for the study will be
accessed by the antimicrobial property of three purified
gandhaka samples obtained after three different types of
shodhana vidhis. Objectives: Physicochemical study of
different methods of gandhaka shodhana and to evaluate
the evaluation its antimicrobial property. To carry out the
shodhana of gandhaka by different methods, to carry out
the physicochemical analysis of samples prepared and to
find out the antimicrobial property of prepared samples.
Materials and Methods: Material which was required for
the shodhana method was procured from local market. For
analytical study and antimicrobial study, material available
in the laboratory was used. Gandhaka purification was
done by Goghrit and Godugdha, Bhrungraj swarasa and Til
Tail. Physicochemical analysis was done before and after
purification of Gandhaka. Antimicrobial study was done
by well diffusion assay. Results: There was a remarkable
difference observed in antimicrobial activity of raw and
shodhita samples of Gandhaka by different methods.
Conclusion: This study concluded that there was the
difference observed between raw and shodhita samples.
UA/OP/55
The technical development of Bahyaswedana (external
sudation) Karma w.s.r Sthanika (Kati, Greeva, Prusta,
Janu and Hrud) Basti
Umesh S. Holennavar, B. S. Prasad, R. V. Sanapeti
Department of Panchakarma, KLEU’s Shri BMK Ayurved
Mahavidyalaya, Belgaum, Karnataka, India
E‑mail: Umesh.holennavar@gmail.com
Background: Ayurveda is an indigenous system of medicine
practicing in Indian sub‑continent since ages. It has a
special mode and modalities of drug administration called as
Panchakarma and some modified pre Panchakarma procedure
Bahyaswedana like sthanika basti‑Kati basti and Jaanubasti.
These procedures are costly and required huge amount of oil
to perform and also time consumption hence modification
of these technique with modern technique hence present
concept has been put forth. Objective: Implementation of
new technology in external sudation therapy with view of
giving good effect of therapy. Materials and Methods: The
Shanikabasti has main procedural component of constant
maintenance of temperature this can be performed by
adopting near‑infrared (NIR) light along with oil. Moreover,
NIR light has a special property of analgesic thus modified
procedure can become worth and cost‑effective procure.
Results: The NIR light has analgesic, wound healing, and
tissue reforming capacity. The NIR disassociates nitric oxide
leads to restoration of adenosine triphosphate production, a
reduction of oxidative stress and subsequently reduction in
inflammation. This can enhance the effect of Sthanikabasti by
performing with oil. This procedure may have few limitations
like selective hypersensitivity to NIR light. Conclusion:
Adaptation of modern technique in Ayurvedic science is a
need of the hour in Ayurveda. Present procedure is one such
[Downloaded free from https://www.jaim.in on Monday, February 15, 2016, IP: 115.112.118.203]
Abstracts
These abstracts were presented at the Update Ayurveda 2014 at Mumbai, Maharashtra, India. The abstracts were screened
by the scientific committee of Update Ayurveda 2014 and are not peer reviewed by J-AIM. © Update Ayurveda 2014.
22
innovative thought, and this can surely cost‑effective and
much more effective than normal Sthanikabasti technique.
UA/OP/56
The ocular hypotensive effect of Gokshuradi guggul
in primary open angle glaucoma: A Pilot Study
A. Patil Ashwini
Department of Shalakyatantra, Dr. D Y Patil College of Ayurved
and Research Institute, Nerul Navi Mumbai, Maharashtra, India
E‑mail: drashwinipatil14@gmail.com
Background: Glaucoma is a group of eye diseases that
damages the optic nerve, which carries information from
the eye to the brain. Glaucoma is estimated to affect 60.5
million persons worldwide by the year 2010. The estimated
prevalence of glaucoma for India is 11.9 million. Glaucoma
damage vision so gradually that nobody notice it until the
disease is at an advanced stage. Either accidentally or
in the advanced stage, glaucoma is diagnosed. This can
permanently damage vision in the affected eye (s) and leads
to blindness if left untreated. It is normally associated with
increased intraocular pressure (IOP). Primary open‑angle
glaucoma (POAG) is one of the most common causes of
irreversible blindness. The progressive nature of glaucoma and
its growing incidence makes its therapy an important target
for research. Herein, we evaluate the effect of Gokshuradi
guggul on IOP in eyes with POAG. Objectives: To evaluate
the ocular hypotensive effect of Gokshuradi guggul in
POAG. Materials and Methods: Patients in Shalakyatantra
out‑patient department and in‑patient department were
recruited in this study after proper consent. Subjects in
this study were treated with Gokshuradi guggul 250 mg 2
bd – 4 weeks. Followed by 2 hs for 8 weeks. Twenty‑four
eyes of clinically stable POAG patients were enrolled in this
pilot study. Results: IOP was significantly reduced after
3 weeks of treatment. Conclusions: Gokshuradi guggul
seems to exert an ocular hypotensive effect in POAG. This
effect became evident after 3 weeks of therapy.
UA/OP/57
Clinical efficacy of Anjana Varti and Vidalaka in
Kaphaja abhishyanda w.s.r. to allergic conjunctivitis
Kirti Kumar Akhand, Babban Prasad, Pankaj Kundal
Department of Shalakya Tantra, National Institute of Ayurveda,
Jaipur, Rajasthan, India
E‑mail: kirti.akhand12@gmail.com
Background: Kaphaja Abhishyanda where itching and
grittiness of the eye, foreign body sensation of eyes,
watering, congestion, eye lid oedema, scanty and ropy
discharge from eyes, heaviness of eyes, etc. It is quite
similar to allergic conjunctivitis is a common ocular ailment
of childhood and adolescent age group as described in the
modern ophthalmology text. Seasonal allergic conjunctivitis
and perennial allergic conjunctivitis accounts for 66–90% of
all cases of allergic eye diseases. Objective: To assess the
efficacy of Anjana varti and Vidalak in the management of
Kaphaja Abhisyanda w.s.r. Allergic conjunctivitis. Materials
and Methods: A total of 45 patients (age of 15–70 years)
were selected randomly 15 in each Group A, B and C. The
selection of patients was done from out‑patient department/
in‑patient department of Shalakya Tantra and Shreedhareeyam
eye unit, N.I.A. Jaipur. Group A, patients were administered
Anjana Varti/Aschyotana for 15 days. Group B, patients
were administered Anjana Varti/Aschyotana and Vidalaka for
15 days. Group C, patients were administered rose water
for 15 days. Results: Group A showed statistically highly
significant improvement (P < 0.001) in I, Lacrimation, Burning
sensation, heaviness of eye, oedema of eye lids and conjuctival
congestion. Group B showed statistically highly significant
improvement (P < 0.001) in I, lacrimation, heaviness of eye,
edema of eyelids and the conjuctival congestion, whereas
Group C showed statistically insignificant improvement in
these symptoms. Conclusion: It was concluded that Anjana
Varti/Aschyotana and Vidalaka were very effective in Kaphaja
Abhishyanda w.s.r. allergic conjunctivitis, while Group C
showed no significant response.
UA/OP/58
Standardization of Khadir chhal churna and its
conversion in modern tooth paste formulation and
evaluation of its antimicrobial activity
Abhimanyu V. Patankar, Amit C. Firke, Sheela Pargunde,
Leena Nandeshwar, V. V. Gurale1, S. R. Kulkarni1
Department of Rasa Shastra and Baishajya Kalpana,
YMT Ayurvedic Medical College, Kharghar, Navi Mumbai,
1Department of Pharmacognosy and Phytochemistry, Bombay College of
Pharmacy, Kalina, Santacruz, Mumbai, Maharashtra, India.
E‑mail: patankarabhimanyu63@gmail.com
Introduction: Dant rog, mukhrog samprapti and chikitsa
have been described in Laghutrayi and Brihatrayi. The
formulations used for the chikitsa are mainly churna
for gharshana and kwath for kawal. Churnakalpana is
a super fine pounded dry herb, which is again sieved
through cloth and Pratisaran Churna is to be rubbed over
teeth in oral cavity. Objective: In the current study, this
is sincere effort to make the toothpaste formulation of
Khadir (acacia catechu) chhal churna. As the herb Khadir
has been advised to use in many of the mukha‑rogas,
here we evaluate the krumighna guna of khadir by in vitro
anti‑microbial activity against Streptococcus mutans.
Materials and Methods: Paste was prepared by using the heat
to soften the base prior to incorporating the solids, which
have been comminuted. The paste was subjected for in vitro
anti‑microbial activity against S. mutans and chlorhexidine
were used as standard (200 μg). Results: Toothpaste shows
zone of inhibition of 2 cm and 2.5 cm at dose of 50 mg and
100 mg respectively. Standard chlorhexidine shows zone of
inhibition 1.5 cm at the dose of (200 μg). Conclusion: Hence,
this study revealed that the modern toothpaste formulation
had good antimicrobial activity.
UA/OP/59
Role of Apamarga Pratisarneeya Kshara in the
management of rectal prolapse: A case study
Aditya Kumar Shil, P. Hemantha Kumar, B. Swapna
Department of Shalya Tantra, National Institute of Ayurveda, Jaipur,
Rajasthan, India
E‑mail: adipbt27@gmail.com
[Downloaded free from https://www.jaim.in on Monday, February 15, 2016, IP: 115.112.118.203]
Abstracts
These abstracts were presented at the Update Ayurveda 2014 at Mumbai, Maharashtra, India. The abstracts were screened
by the scientific committee of Update Ayurveda 2014 and are not peer reviewed by J-AIM. © Update Ayurveda 2014.
23
Background: Kshara karma is the para‑surgical procedure
described in Ayurvedic classics. As the surgery is an ultimate
treatment in rectal prolapse with its own limitations. Kshara
Karma can be recommended as an emerging alternative to
surgery with minimal postprocedure complications. Objective:
To develop a cost‑effective, conducive para‑surgical procedure
in the management of rectal prolapse. Materials and Methods:
This study was conducted on a female patient aged 48 years
who was diagnosed as complete rectal prolapse, admitted
in Shalya Tantra in‑patient department of our institute.
Procedure was performed under local anesthesia. After
reducing the prolapsed rectum the Apamarga Pratisarneeya
Kshara was applied circumferentially on the rectal mucosa by
using a slit proctoscope and applicator. Kshara was applied
for 2 min (100 Matrakala) till the color of rectal mucosa was
changed to Pakwvajambuphalavarna, then the area was
washed with distilled water, followed by Nimbuswarasa. The
process was repeated 4 times to cover whole circumference
of the rectal mucosa. Matravasti with Yashtimadhughrita
was given twice daily for 7 days from first postprocedure
day, followed by with Changerighrita twice daily for 15 days.
Patient was advised pathyaapthyaaahara‑vihara. Results:
Patient had relief from symptoms of rectal prolapse. No rectal
prolapse was complained during follow‑up period. Conclusion:
Kshara karma is a conducive, day care procedure and is very
cost‑effective, which can be performed under local anesthesia
with minimal and trivial postprocedure complications which
are negligible and can be recommended as an emerging
alternative to surgery.
UA/OP/60
Understanding congestive cardiac failure by
pulse – Nadipariksha
Anil Digambar Vaidya
Ayurveda Clinic Marve, Mumbai,
E‑mail: drvaidya46@yahoo.com
Background: Pulse is one of the parameters for heart
working. Using Nadipariksha reduction in pumping of the
heart in congestive cardiac failure (CCF) can be graded from
0 to 15, where 0 indicates 100% pumping. According to
gradation, we can understand the condition of the heart
and thereby improvement in patient health state. Also,
through this method we can differentiate CCF into left
or right ventricular failure. Objectives: To prove relation
between the pulse and reduction in pumping of the heart,
to judge improvement in patient by pulse gradation and to
correlate the pulse gradation with left ventricular ejection
factor (LVEF). Materials and Methods: A total of 151
subjects of heart disease including CCF, other noncuring
heart diseases and from relapse of previous illnesses
like ischemic heart disease were studied on out‑patient
department basis for last 20 years. Improvement in CCF
patients was judged by gradation, which correlates with
disappearing signs symptoms and improvement in health.
Patients with noncuring diseases, e.g., bone fracture,
giddiness, Amavat were diagnosed as CCF by their pulse
and were treated with Ayurvedic medicines along with
treatment of the disease. Results: The diagnosis was done
by pulse gradation and echo‑cardiogram. Improvement after
treatment was observed in health status and as judged by
pulse gradation. LVEF did not show improvement in aortic
regurgitation and weakness. Conclusion: The pulse is
definitely effective in diagnosing CCF. Its gradation is useful
in understanding heart’s power and health improvement.
Pulse gradation can be of clinical significance.
UA/OP/61
Effect of Painmukti MJ tablets, Painmukti cream and
Painmukti‑sandhical tablets in patients suffering from
chronic pain (including arthritis)
Renuka Munshi, Dipti Kumbhar, Supriya Bhalerao,
Jairam Jagasia1, Ashish Parikh1
Departments of Clinical Pharmacology and 1Orthopedics, TNMC and
BYL Nair Ch. Hospital, Mumbai, Maharashtra, India
E‑mail: dipti.wadangekar@gmail.com
Background: Common chronic pain conditions include
headache, low back pain and arthritic pain. Although there
are many analgesics available, their chronic use can lead
to adverse effects on the body. Ayurveda recommends
various preparations for the management of pain. Painmukti
MJ tablets contain herbs like Nirgundhi, Rasna, Shallaki
and Shunthi while Painmukti‑Sandhical tablets contain
Asthishrunkhla, Lakha, Aswagandha, Mukta. Objective: To
evaluate comparative efficacy and safety of Painmukti MJ
tablets, Painmukti‑cream and Painmukti‑sandhical tablets
in patients with chronic pain. Materials and Methods: This
study was initiated after Institutional Ethics Committee
approval. Patients with chronic pain lasting >3 months
were randomized to one of 3 groups and received either
tablet Painmukti MJ + Painmukti cream (Group I), tablet
Painmukti MJ + Painmukti‑sandhical tablets + Painmukti
cream (Group II) and placebo tablets + diclofenac
gel (Group III) for 3 months. Monthly assessments included
pain relief using modified Western Ontario and McMaster
Universities Arthritis Index (WOMAC), visual analog
scales (VAS), Quality of life using EQ‑five‑dimensions
questionnaire and analgesic requirement. Results: Totally
106 patients were recruited (85 with arthritis) of which
2 patients were withdrawn following adverse events.
Significant improvement in pain score (WOMAC and VAS)
was seen in both Groups I and II at the end of 3 months
of therapy as compared to the baseline (34–21 in Group I;
33–24 in Group II and 36–26 in Group III). Quality of life
improved significantly in Group I with a decreased need
for analgesics. Conclusion: Thus, Painmukti and Sandhical
medications, which contain a combination of herbs with
analgesic and anti‑inflammatory properties, possess qualities
that enhance agni, pacify ama and vitiate vata dosha.
UA/OP/62
Evaluation of the anti‑depressant activity of the
aqueous extract of Tinospora cordifolia in two
experimental models of depression using behavioral
and biochemical parameters
Renuka P. Munshi, Bhagyeshri N. Rane, Deepika N. Kshirsagar
Department of Clinical Pharmacology, TNMC and BYL Nair Ch. Hospital,
Mumbai, Maharashtra, India
E‑mail: dkkulkarni10@gmail.com
[Downloaded free from https://www.jaim.in on Monday, February 15, 2016, IP: 115.112.118.203]
Abstracts
These abstracts were presented at the Update Ayurveda 2014 at Mumbai, Maharashtra, India. The abstracts were screened
by the scientific committee of Update Ayurveda 2014 and are not peer reviewed by J-AIM. © Update Ayurveda 2014.
24
Background: Depression is referred to as the common
cold of psychiatry, but it makes light impact of an illness
that can steal from a person his ability to conduct and
enjoy a normal life. Current treatment provides some
improvement in clinical condition of patients with burden
of their adverse effects hence need to explore alternatives
from Indian medicinal system that are effective and
safe. Objective: To explore antidepressant potential of
Tinospora cordifolia (TC) by exploring its role on the
neurotransmitters – serotonin, acetylcholine esterase and
monoamine oxidase (MAO) enzymes. Methods: Wistar
rats were evaluated for anti‑depressant activity using tail
suspension test (TST) and forced swimming test (FST)
in separate groups. Fluoxetine: 20 mg/kg/day and test
drug (crude aqueous extract of TC 400 mg/kg/day) was
administered orally for 21 successive days, 1‑h prior
to subjecting the groups to respective behavioral tests.
Blood serotonin was analyzed on weekly intervals. On day
21, animals were sacrificed and the excised brain was
assessed for serotonin, acetyl‑cholinesterase (ACh) activity,
and MAO‑A and MAO‑B levels. Results: A significant
decrease in immobility period (1.835 ± 0.61 and
2.8075 ± 0.39) in both models. Significant increase in
blood (9.552 ± 0.366 and 12.331 ± 0.306) and brain
serotonin (0.866 ± 0.021 and 0.895 ± 0.056) levels,
significant diminution in brain ACh (0.0543 ± 0.003
and 0.0439 ± 0.0113); MAO‑A (23.79 ± 1.06 and
26.61 ± 1.10) and MAO‑B (8.33 ± 1.80 and 8.74 ± 0.89)
levels was observed in TST and FST group, demonstrating
anti‑depressant activity. Conclusion: TC modulated altered
neurotransmitter and enzyme levels towards normal
demonstrating anti‑depressant effect.
UA/OP/63
Pharmacological evaluation of anti‑inflammatory
and analgesic activity of Blepharispermum
subsessile DC root on albino rats
Amruta Jadhav, R. N. Acharya1, Mukesh Nariya2
Department of Dravyaguna, DY Patil School of Ayurveda, Nerul,
Navi Mumbai, Maharashtra, India, 1Department of Dravyaguna,
I.P.G.T. and R.A., Gujarat Ayurved University, 2Pharmacology lab,
I.P.G.T. and R.A., Gujarat Ayurved University, Jamnagar, Gujarat, India
E‑mail: dramruta113@gmail.com
Background: In Ayurveda the root of Rasna is a most frequently
used herbal drug for the management of vaticdisorders (pain
and inflammation). The root Blepharispermum subsessile
DC (Asteraceae), known as Rasna by the traditional
practitioner of Odisha and Chhattisgarh region of India, is
marketed with a highest price as Rasna in this region. Until
now, the drug is not evaluated for its therapeutic claim in a
scientific way. Objective: To evaluate the anti‑inflammatory
and analgesic effect of root of B. subsessile. Materials
and Methods: The decoction of B. subsessile root (4.5 ml/
kg), was evaluated on Wister strain albino rats, for its
anti‑inflammatory (carrageenan and formalin‑induced paw
edema) and Analgesic activity (tail flick and formalin‑induced
paw licking method). Results: Decoction of B. subsessile
inhibited both the phases of carrageenan‑induced
inflammatory responses significantly compared to the control
group. However, in formalin‑induced paw edema model the
drug showed moderate anti‑inflammatory response. In the
formalin‑induced paw licking both test drugs enhanced
the latency of onset of paw licking as well as decrease in
number of formalin‑induced paw licking. Though the result
found is not statistically significant but the percentage wise
decrease in number of paw licking is suggestive that the test
drugs have mild inhibitory activity against formalin‑induced
inflammatory pain while the drug is having minimal effect
against radiant heat‑induced pain in rats compared to
control group. Conclusion: DC B. subsessile root possesses
significant anti‑inflammatory and mild analgesic activity
while tested on Wister strain albino rats.
UA/OP/64
Effect of Ashwagandharishta on learning and memory
in experimental models of scopolamine induced
amnesia
S. Shirolkar, S. B. Raut, Rao Rajendra Prasad, P. A. Marathe,
N. Rege Nirmala
Department of Pharmacology and Therapeutics, Seth GS
Medical College and KEM Hospital, Mumbai, Maharashtra, India
E‑mail: rajendra.rajen74@gmail.com
Background: Ashwagandharishta (ASH), a polyherbal
formulation is recommended in Ayurveda for loss of memory,
weight loss, fainting, headache, psychosis, senile debility.
Effects of ASH on memory have not been studied before.
Objective: To evaluate the effect of ASH on learning and
memory using scopolamine‑induced memory impairment in
animals. Materials and Methods: Following Animal Ethics
Committee permission, 18 Wistar rats and 18 Swiss albino
mice were used in two behavioral paradigms; Morris water
maze (MWM) and elevated plus maze (EPM) respectively.
Each experimental model had three groups of 6 animals each
such as distilled water control, rivastigmine (2.4 mg/kg/day)
as positive control and ASH (0.13 ml/kg to mice and
4.9 ml/kg to rats). All drugs were administered orally for
15 days. Scopolamine was given to all animals in the
dose of 3 mg/kg I.P. to induce amnesia 90 min after the
last dose on 15th day. Training was started 45 min after
scopolamine administration in MWM for rats and EPM for
mice. Twenty‑four hours later, memory of the animals
was assessed using escape latency (MWM) and transfer
latency (EPM). Data was analyzed using ANOVA with
Tukey’s post‑hoc test (P < 0.05). Results: ASH showed
statistically significant results as compared to vehicle
control and comparable findings to rivastigmine in both the
experiments (P < 0.05). Conclusion: Administration of ASH
for 15 consecutive days significantly improved memory and
learning of experimental animals. ASH may be considered
as a promising agent for enhancing learning and memory.
UA/OP/65
Effect of systemic Ayurvedic medicines in management
of branch retinal vein occlusion with chronic macular
edema: A case study
Dipti Nene
Department of Shalaya Tantra, DY Patil School of Ayurveda, Navi
Mumbai, Maharashtra, India. E‑mail: dipti.nene@gmail.com
[Downloaded free from https://www.jaim.in on Monday, February 15, 2016, IP: 115.112.118.203]
Abstracts
These abstracts were presented at the Update Ayurveda 2014 at Mumbai, Maharashtra, India. The abstracts were screened
by the scientific committee of Update Ayurveda 2014 and are not peer reviewed by J-AIM. © Update Ayurveda 2014.
25
Background: Branch retinal vein occlusion (BRVO) is retinal
vascular disease. Arteriosclerosis and hypertension are its
predisposing factors. It presents as sudden onset of painless
unilateral visual field defect, metamorphopsia. It complicates
into chronic macular edema (CME) leading to poor visual acuity.
Though pan‑retinal photocoagulation (PRP) is the treatment
for CME, it is not much beneficial in chronic cases. There is a
need for Ayurvedic solutions in chronic cases such as BRVO
and CME. Objectives: To explore pathogenesis in Ayurvedic
terms and to find out Ayurvedic treatment for this condition.
Methodology: A 59‑year‑old male patient (known hypertensive)
came to out‑patient department for diminished vision of right
eye (6/60 and 6/60 with glasses), central scotoma, abnormal
color vision and metamorphopsia. He had a sudden onset
of these symptoms 17 months back. He was previously
diagnosed as BRVO and CME and treated with topical steroids
followed by intra‑ocular Avastin injection twice. PRP was
also done. Patient then approached for Ayurvedic treatment.
After drawing suitable Ayurvedic pathogenesis (samprapti)
by ashtavidha parikshan, following treatment was given:
Oral pittashaman chikitsa and anuloman dravya and local
bidalak (application of herbal paste over eyelid) for 3 months.
Results: The distortion of images (metamorphopsia) was
significantly reduced as recorded by Amsler grid. Before
treatment visual acuity of affected (right) eye was 6/60 and
6/60 with glasses. After treatment visual acuity of affected eye
was 6/18 and 6/9 with glasses. There was an improvement in
near vision and color vision. Conclusion: Systematic Ayurvedic
treatment is effective in BRVO with CME.
UA/OP/66
Clinical study of psoriasis research
Vikram Pandit
Aparna Clinic, Solapur, Maharashtra, India
E‑mail: researchvikram@yahoo.co.in
Background: Psoriasis is a noncontiguous inflammatory
skin diseases characterized by recurring reddish patches
covered with silvery scales. Symptoms and signs of
psoriasis resembles with kitibh kushtha as mentioned
in Ayurveda. 8% of the world’s population is suffering
from psoriasis. The changes occurring in the skin due to
psoriasis can be reverted by treatment with Ayurvedic
medicinal plants. Objectives: To evaluate the effect of
Ayurvedic drug regimen in the management of psoriasis.
Materials and Methods: A total of 25 patients of psoriasis
were selected for the study and individual consent was
taken. Ayurvedic drug regimen consisting of Tinospora
cardiofolia 500 mg bd, Withania somnifera 500 mg hs,
Azadirakta indica, Neem leaves Churna 100 mg daily after
lunch, external application of Neem soap to reduce itching,
Shatadhauta Ghrita and Aloe vera for local application,
Aloe vera preparation like kumari asava (200 ml before
meal) if the liver function was abnormal was advised to
the patients. The patients were also asked to listen to
music especially kedar raga in the evening (6 p.m.–8 p.m.).
10 patients having low Vitamin B12 levels were advised to
take 500 ml cow milk daily against treatment for Vitamin
B12 deficiency and calcium deficiency. Pathya apathya
was prescribed as per Charak Samhita. Hematological
investigations viz. Serum IgE, serum IgM, serum IgA and
routine biochemicals were done before and after treatment.
Results: Ayurvedic drug regimen gave promising result in
improving psoriasis. Vitamin B12 deficiency in psoriasis
was resolved by Cow’s milk. Improvement in ejection
fraction values was observed. Also, punch biopsy was
done to observe histopathological changes. There was
hematological and histopathological reversion. Conclusion:
Psoriasis can be treated with our Indian medicinal
plants Ayurveda siddhanta and hematological reversion.
Histo‑pathological reversion is the final answer for this
autoimmune disease.
Description
Keywords
Citation
Update Ayurveda 2014 Abstracts Oral Presentation. Journal of Ayurveda and Integrative Medicine. 2015 May; 6(suppl_5): 5-25.