Enzyme Based Asparagine Biosensor for the Detection of Asparagine Levels in Leukemic Samples.
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Date
2015-10
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Abstract
Plants have been used for the treatment of different diseases since 1100 BC. Catharanthus
roseus is one of the medicinally important plants showing anti-tumor, anti-mutagenic and anti-microbial effects.
L-asparaginase is extracted by C. roseus and is used for the development of biosensors. L-asparaginase breaks
down asparagine into aspartic acid and ammonia, thus killing the tumor cells due to asparagine starvation. In the
present study the L-asparaginase was extracted from C. roseus and immobilized in different matrixes such as
agar, soil, clay and k-carrageenan. Out of the various immobilization techniques k-carrageenan gave the fastest
response time, so it was choosen for the development of asparagine biosensor. In the leukemic blood samples
the asparagine concentration was 10-2 to 10-3 M whereas in normal blood samples it was 10-5 to 10-6 M. The
developed biosensor gave the fast response and were sensitive and reliable.
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Keywords
L-asparaginase, immobilization, leukemia, k-carrageenan, C. roseus
Citation
Punia Sandeep, Kumar Raman, Kumar Kuldeep. Enzyme Based Asparagine Biosensor for the Detection of Asparagine Levels in Leukemic Samples. International Journal of Applied Biology and Pharmaceutical Technology. 2015 Oct-Dec; 6(4): 40-43.