Ureide biogenesis and the enzymes of ammonia assimilation and ureide biosynthesis in nitrogen fixing pigeonpea (Cajanus cajan) nodules.
Loading...
Date
1984-06
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Allantoic acid production from IMP, XMP, inosine, xanthosine, hypoxanthine,
xanthine, uric acid and allantoin was investigated by incubating each of these substrates with
Cajanus cajan cytosol and bacteroid fractions separately in the presence and absence of NAD+
and allopurinol. Allantoic acid synthesis by bacteroid fraction could only be observed with uric
acid and allantoin as substrates. Addition of NAD+ or allopurinol to the reaction mixtures had
no effect. However, with cytosol fraction, allantoic acid was produced by each of these
substrates, with maximum rate with allantoin. With NAD+ or with allopurinol, allantoic acid
was produced only with uric acid and allantoin as substrates. NADH production with cytosol
fraction could again be observed with all the substrates. Except with uric acid and allantoin,
allopurinol completely inhibited NADH formation. Regardless of the presence or absence of
allopurinol, none of the substrates exhibited significant activity with bacteroid fraction. Based
on the activities of glutamine synthetase, glutamate synthase, glutamate dehydrogenase,
aspartate aminotransferase, asparagine synthetase, nucleotidase, nucleosidase, xanthine dehydrogenase,
uricase and allantoinase and their intracellular localisation in various nodule
fractions, a probable pathway for the biogenesis of ureides in pigeonpea nodules has been
proposed.
Description
Keywords
Pigeonpea, Cajanus cajan, nodules, ureide biogenesis, intracellular location
Citation
Amarjit, Singh Randhir. Ureide biogenesis and the enzymes of ammonia assimilation and ureide biosynthesis in nitrogen fixing pigeonpea (Cajanus cajan) nodules. Journal of Biosciences. 1984 June; 6(2): 185-192.