Study of virulence factors of uropathogenic Escherichia coli and its antibiotic susceptibility pattern.
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Date
2014-01
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Abstract
Context: Urinary tract infection (UTI) is one of the most common nosocomial
infections, caused by Escherichia coli. This study determined the presence
of virulence factors in the organism and correlates it with the multi-drug
resistance (MDR). Aims: The aim of the following study is to assess the
virulence factors of uropathogenic E. coli and antibiotic susceptibility
pattern. Settings and Design: This was a prospective study conducted
in the Department of Microbiology in PT. B. D. Sharma, PGIMS, Rohtak.
Subjects and Methods: The study was conducted over a period of 1 year.
Urine samples received were processed as per standard microbiological
procedures. Virulence factors such as hemolysin, hemagglutination, cell surface
hydrophobicity, serum resistance, gelatinase and siderophore production
were studied. The antimicrobial susceptibility was done as per Clinical and
Laboratory Standard Institute Guidelines. Statistical Analysis Used: The data
was analyzed by using SPSS(Statistical Package for the social sciences) IBM
Corporation version 17.0. A two sided P ≤ 0.05 was considered to be signifi cant.
Results: Hemolysin production was seen in 47.4%, hemagglutination in 74.8%,
cell surface hydrophobicity in 61%, serum resistance in 59%, gelatinase in 67.5%
and siderophore production in 88% isolates. Nitrofurantoin was found to be most
effective followed by, gatifl oxacin and gentamicin. Twenty nine percent (29.62%)
isolates were MDR. Conclusions: Therefore, the knowledge of virulence factors
of E. coli and their antibiotic susceptibility pattern will help in better understanding
of the organism and in the treatment of UTI.
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Antibiotic susceptibility, multiple drug resistance, uropathogenic Escherichia coli, virulence factors
Citation
Mittal Seema, Sharma Madhu, Chaudhary Uma. Study of virulence factors of uropathogenic Escherichia coli and its antibiotic susceptibility pattern. Indian Journal of Pathology & Microbiology. 2014 Jan-Mar 57 (1): 61-64.