Studies on influence of natural biowastes on cellulose production by Aspergillus niger.
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Date
2011-11
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Abstract
The objective of this study was to determine the influence of natural biowaste substrates such as banana peel
powder and coir powder at varying environmental parameters of pH (4-9) and temperature (20-50oC) on the
cellulase enzyme production by Aspergillus niger. The cellulase enzyme production was analyzed by
measuring the amount of glucose liberated in IU ml-1 by using the dinitrosalicylic acid assay method. The
substrates were pretreated with 1% NaOH (alkaline treatment) and autoclaved. The maximum activity of the
enzyme was assayed at varying pH with temperatures being constant and varying temperatures with pH
being constant. The highest activity of the enzyme at varying pH was recorded at pH 6 for banana peel
powder (0.068 + 0.002 IU ml-1) and coir powder (0.049 ± 0.002 IU ml-1) and the maximum activity of the
enzyme at varying temperature was recorded at 35oC for both banana peel powder (0.072 ±0.001 IU ml-1)
and coir powder (0.046 ±0.003 IU ml-1). At varying temperatures and pH the high level of enzyme production
was obtained at 35oC and pH 6 by using both the substrates, respectively. However among the two
substrates used for the production of cellulases by Aspergillus niger banana peel powder showed maximum
enzymatic activity than coir powder as substrate.
Description
Keywords
Natural biowaste, Cellulase activity, Aspergillus niger
Citation
Kiranmayi M Usha, Poda Sudhakar, Charyulu P B B N, Vijayalakshmi M, Krishna P V. Studies on influence of natural biowastes on cellulose production by Aspergillus niger. Journal of Environmental Biology. 2011 Nov; 32(6): 695-699.