Detection of p53 codon 72 gene polymorphism in patients with oral cancer
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Date
2010-04-06
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Thai Cancer Journal
Abstract
Oral cancer is a serious malignant disease that caused vastly losses in Thailand annum. The potential risk factor for predicting and screening of high-risk populations that developed early stage oral cancer followed by immediately intensive counseling and efficiency treatment is an important strategy to control this harmful cancer. To address on the genetic risk factor for oral cancer was investigated. The association between the p53 codon 72 gene polymorphism and oral cancer susceptibility in Thai people. The frequency of p53 codon 72 gene polymorphism (Arginine/Arginine, Arginine/Proline and Proline/Proline genotypes) in 80 oral cancer patients, 80 chronic oral disease patients and 80 age-matched healthy controls was determined by using a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay. Statistically significant difference in the overall genotype frequencies between cases and controls comprising chronic oral disease patients and healthy controls was observed (p \< 0.05). Proline/Proline genotype carriers had 2.8-fold increased risk for oral cancer as compared with Arginine/Arginine genotype carriers (Odds ratio = 2.8, 95% confidence interval = 1.0-4.7). Among oral cancer patients, statistical significant difference in p53 genotype frequencies between clinical stages was also observed. The results in this study suggest that the p53 codon 72 gene polymorphism may associate with oral cancer susceptibility in Thai population, particularly the Proline/Proline genotype carrier. The suggestion is that the detection of p53 polymorphism may be a useful tool for screening of the high-risk group as well as prognosis of oral cancer in Thai people.
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Citation
Thai Cancer Journal; Vol.28 No.1 January-March 2008; 24-33