Monoclonal antibodies to microfilariae of Wuchereria bancrofti
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Date
1992
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Publisher
University of Colombo: UC(MED).
Abstract
A panel of mouse monoclonal antibodies (Mabs) against microfilariae of Wuchereria bancrofti were produced and designated as MfWb1. MfWb1 was stage and species specific to microfilariae of W. bancrofti. Specificities were established by the indirect fluorescence antibody test (IFAT). There was no reactivity in the IFAT against infective larvae of W. bancrofti, microfilariae of Brugia pahangi, microfilariae of Brugia malayi, adult Onchocerca gibsoni, adult O. gutturosa and Acanthocheilonema viteae. MfWb1 reacted only with the surface antigens on the sheath of microfilariae of W. bancrofti and the location was determined by IFAT and immunocytochemistry. Three prominent antigenic polypeptide bands and one less prominent band with molecular weights ranging from 95 to 230 kDa were identified by MfWb1 on western blots. By immunoprecipitation, seven common and highly immunogenic parasitic surface antigens were recognized by MfWb1. The range of relative molecular weights of these antigens were from 30 kDa to 60 kDa. The functional significance of MfWb1 wa also assessed by performing feeding experiments using laboratory bred Aedes togoi and it was found that MfWb1 has an effect on the transmission of W. bancrofti. The infection rate in MfWb1 fed mosquitoes was lower when compared to controls. At lower dilutions of up to 1 : 6, MfWb1 completely inhibited the development of microfilariae in infected mosquitoes. This effect starts to decrease from a dilution of 1 : 10. It is also probable that the MfWb1 reacts on the sheath of the microfilaria after a blood meal and decreases the infectivity within the 1st six days. Development of various immunodiagnostic tests for the detection of infected patients with bancroftian filariasis was attempted by using MfWb1. An ELISA incorporating MfWb1 was attempted. The sensitivity of the ELISA assay that was attempted was inadequate to detect circulating antigens in infected patients. Though the dot blot assay and the inhibitory IFAT were useful to detect some of the infected patients, none gave a 100 percent positivity. However the dot blot assay was more sensitive than the inhibitory IFAT for the detection of microfilaraemic patients.
Description
Dissertation: M.Phil., University of Colombo: UC(MED), 1992.
Keywords
Filarioidea
Citation
JAYAWARDANA, KH, Monoclonal antibodies to microfilariae of Wuchereria bancrofti, University of Colombo UC(MED), 1992: 138p.