Microbiology Research Journal International
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Chief Editor: Prof. En Tao Wang
ISSN: 2456-7043
Frequency: Quarterly
Language: English
Open Access Peer-reviewed journal
Web site: https://www.journalmrji.com/index.php/MRJI
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Browsing Microbiology Research Journal International by Subject "16S rDNA"
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Item Isolation and Identification of Streptomyces rochei Strain Active against Phytopathogenic Fungi.(2014-10) El Hussein, Adil A; Alhasan, Rihab E M; Abdelwahab, Suhair A; El Siddig, Marmar AA total of 104 actinomycete isolates were recovered from farming soil samples collected from 11 states in Sudan. Upon screening for potential antifungal activity, an actinomycete isolate (R92) was found to be highly antagonistic against all of the tested phytopathogenic fungi. It was identified as Streptomyces rochei on the basis of its morphology, chemotaxonomy and 16S rDNA sequence analysis. In vivo antagonistic activities of the nbutanol extract of R92 culture were significant since the progress of Drechslera halodes leaf spot on sorghum and Alternaria alternata early blight on tomato was highly restricted and incidence of both diseases was greatly suppressed. Trials to evaluate the In vivo control efficacy of this extract under field conditions is recommended.Item Phenotypic and Genotypic Characteristics of Rhizobia Isolated from Meknes-tafilalet Soils and Study of Their Ability to Nodulate Bituminaria bituminosa.(2014-04) Messaoud, Btissam Ben; Aboumerieme, Imane; Nassiri, Laila; Fahime, Elmostafa El; Ibijbijen, JamalAims: The objectives were to isolate and characterize phenotypically and genotypically the rhizobial strains from the soils belonging to the Meknes-Tafilalet region in order to select strains that are able to nodulate Bituminaria bituminosa. Study Design: An experimental study. Place and Duration of Study: Department of biology (Soil & Environment Microbiology Unit) Faculty of Sciences, Moulay Ismail University and Technical Support Unit for Scientific Research, CNRST in Rabat; between January and August 2010. Methodology: Samples from 23 different sites belonging to the Meknes-Tafilalet region were collected in order to select rhizobial strains that are able to nodulate Bituminaria bituminosa. The morphological, cultural and phenotypic parameters of isolated strains were studied. The phenotypic characteristics include colony morphology, growth speed, tolerances to temperature, salt and pH. To assess the genotypic diversity among the isolates, molecular characteristics based on 16S rDNA gene sequencing were performed. Results: The majority of the isolated strains showed fast-growing capacity (75%). Most strains tolerate neutral to alkaline pH, however some strains (18%) showed weak growth capacity at pH 4. All isolates were tolerant to high salt stress ([NaCl] = 3%). The genotypic characterization based on16S rDNA gene sequencing of the twelve strains showed a high diversity between the isolates. Conclusion: Taken together, our results highlight the important biodiversity of the isolated rhizobial strains and open opportunities for the development of new bio-fertilizer.