Browsing by Author "Yoshida, Hitomi"
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Item Ethanol extract of rosemary repressed pten expression in K562 culture cells.(2011-01) Yoshida, Hitomi; Okumura, Naoko; Kitagishi, Yasuko; Nishimura, Yuri; Matsuda, SatoruSeveral medicinal herbs are found to have a potent transcriptional activity for the gene involved in cellular function. In the present study, we aimed to determine the effects of such herbs on PTEN (phosphatase and tensin homolog deleted on chromosome 10) gene, which is a candidate gene involved in cancer development. The in vitro effect of a Rosemary (Rosmarinus officinalis) extract on the reduction of PTEN expression was confirmed by reverse transcriptase-polymerase chain reaction (RT-PCR). In addition, western blotting also confirmed the down-regulation of the protein at dose dependent manner of the Rosemary extract. Our results show that component(s) of Rosemary inhibits the expression of PTEN in K562 myeloid cell line cells.Item Genistein diminished CIZ1 expression in daudi lymphoid cells.(2010-11) Okumura, Naoko; Nagata, Yuka; Yoshida, Hitomi; Nishimura, Yuri; Kitagishi, Yasuko; Matsuda, SatoruCip-interacting zinc finger protein 1 (Ciz1), stimulates DNA replication and has been implicated in tumorigenesis of breast cancer cells. In order to investigate the possibility of using medicinal isoflavone agaist breast cancer, we studied whether some isoflavones could affect the expression of the Ciz1. The in vitro effect of isoflavone treatment on the reduction of Ciz1 expression was detected by reverse transcriptase-polymerase chain reaction (RT-PCR). Western blotting also confirmed the down-regulation of the protein at dose dependent manner of the genistein treatment in Daudi lymphoid cell line cells.