Browsing by Author "Singh, B K"
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Item Abnormal foreign body in the orbit.(1987-05-01) Verma, R; Singh, B KItem Application of polymerase chain reaction (PCR) for diagnosis of equine herpes virus-1 (EHV-1).(1996-11-01) Gupta, A K; Singh, B K; Yadav, M PFifty aborted foetus samples were diagnosed for the presence of equine herpes virus-1 (EHV-1) by polymerase chain reaction (PCR) technique. Specific primer pair for amplification of a particular segment of EHV-1 DNA in gc region having 3 Hae III restriction endonuclease sites was used. A 409 base pair segment obtained as PCR amplification product in 9 samples was digested with Hae III to confirm the presence of EHV-1 as the infectious agent in aborted tissues. It was observed that PCR technique was more sensitive, specific and rapid than the conventional virological diagnostic methods.Item Cryptosporidium infection in children with acute diarrhoea.(1988-06-01) Singh, B K; Gulati, A K; Prasad, K N; Singla, P NItem Interactions between the renin-angiotensin system and dyslipidemia: relevance in atherogenesis and therapy of coronary heart disease.(2001-07-05) Singh, B K; Mehta, J LHypertension and hypercholesterolemia, two major risk factors for atherosclerotic disease, frequently coexist in patients with hypertension and CAD. Data from clinical studies suggest the existence of lipoprotein-neurohormonal interactions that may adversely affect vascular structure and reactivity. Data from preclinical studies suggest that RAS may be upregulated by abnormal lipids, most likely via production of ox-LDL. On the other hand, activation of RAS leads to release of ROS and transcriptional upregulation of LDL and ox-LDL uptake in macrophages, smooth muscle cells and endothelial cells. These findings extend our understanding of the interplay among risk factors to synergistically increase cardiovascular risk, and of the anti-atherosclerotic effects of local ACE inhibition to reduce cardiovascular risk. Trials aimed at modifying RAS along with drugs lowering total- and LDL-cholesterol levels and inhibitors of oxidative modification of LDL-cholesterol will address the clinical relevance of this biological interaction.Item Production of monoclonal antibodies against equine influenza A/Equi-2 (H3N8) Indian isolate.(1993-09-01) Singh, B K; Sinclair, R; Gupta, A K; Uppal, P K; Yadav, M P; Mumford, J ASeven hybrid cell lines of mouse myeloma cell line NSO and spleen cells of BALB/c mice producing monoclonal antibodies (MAbs) against equine influenza A/Equi-2/Ludhiana/87 (H3N8) virus were developed. These MAbs were purified, isotyped and characterised by enzyme linked immunosorbent assay (ELISA), fluorescent antibody test (FAT), haemagglutination inhibition (HI) and virus neutralization (VN) tests. The titres of ascitic fluids induced by hybridomas as estimated by ELISA ranged from 1:25,600 to 1:51,200. Monoclonality of these clones was confirmed using a panel of 5 viral antigens, each belonging to a single isotype. MAbs (5) belonged to IgM and one each to IgG1 and IgG2a. Two epitopes appeared to be closely resembling by HI and VN tests but other two epitopes appeared to be different.Item Rib tip syndrome.(1981-10-01) Razaque, M A; Singh, B K