Browsing by Author "Sharma, R.K."
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Item Comparison of Wound Healing Properties of Herbal Ointments with Povidone iodine on Basis of Histological Changes(Association of Mastitis, 2019-02) Sharma, Neha; Singh, Randhir; Jawre, Shobha; Vaish, Rakhi; Sharma, R.K.Present study was conducted on 24 dogs, aged between 1 to 8 years, irrespective of sex, breed. The animals were randomly divided in to four groups. In group I, animals were treated with standard dressing material Povidone iodine (5.0%). In animals of group II, III, IV herbal ointments (10% w/w) of hydroalcoholic extract of Marigold leaves, Guava leaves and mixture of both Marigold and Guava leaves extracts respectively were used for dressings of wounds for 7 consecutive days. In animals of group IV, marked re-epithelialization and moderate inflammatory cells, neovascularization and thicker but scattered collagen fibrils were seen on day 7 and mature collagen fibres were observed with least number of inflammatory cells on day 14 as compared to other three groups. Therefore, it can be concluded that group IV (mixture of Marigold and Guava leaves hydroalcoholic extract) revealed better wound healing properties as compared to group I (Povidone iodine 5.0%), group II (Marigold leaves hydroalcoholic extract) and group III (Guava leaves hydroalcoholic extract).Item Effect of Eugenia jambolana and Psidium guajava Leaf Meal Mixture Supplementation on Performance, Biochemical Profile and Histopathological Changes of Broiler Chicks(Association of Mastitis, 2020-04) Zargar, M.A.; Pathak, A.K.; Rahman, S.; Sharma, R.K.; Daing, M.I.Present study was undertaken in 120 day-old broiler chicks, randomly distributed into 4 dietary treatments (T1, T2, T3 and T4 supplemented with leaf meal mixture (LMM) of Eugenia jambolana and Psidium guajava @ 0, 2.5, 5.0 and 7.5 % of diet, respectively) each having 3 replicates (10 chicks/ replicate) in a complete randomized block design (CRD). Experimental chicks were fed basal diets (starter and finisher) supplemented with graded levels of LMM. Feed intake, body weight gain and feed conversion ratio (FCR) were recorded at weekly intervals. Two birds from each replicate were slaughtered at the end of feeding trial (42 days). Blood samples were collected and serum separated for biochemical profiles. For histopathological examination, representative tissue samples were collected in 10% neutral buffer formalin and then processed for paraffin embedding employing alcohol as dehydrating agent and xylene as clearing agent. Sections were cut at 4-5µm thickness and stained by routine haematoxylin and eosin method. On histopathological examination of liver, kidney, heart and intestine of T1 and T2 groups showed normal integrity, mild to moderate histopathological changes in T3 group, while, T4 showed drastic histopathological changes. It was concluded that E. jambolana and P. guajava LMM supplementation (2.5% or even 5%) maintained birds performance, minor histopathological changes and producing healthy low cholesterol broiler meat. The LMM incorporation (2.5%) in the diet of broiler chicks may be recommended as socioeconomic, alternative functional feed resourceItem Evaluation of Wound Healing Properties of Herbal Medicaments on the Basis of Clinical and Haemato-biochemical Changes(Association of Mastitis, 2020-02) Sharma, Neha; Singh, Randhir; Shahi, Apra; Sharma, R.K.; Vaish, Rakhi; Vandre, R.K.The present study was conducted on 24 dogs irrespective of sex, breed and aged between 1year to 8 years with different types of wounds. The animals were randomly divided into four groups. Each group consisted of 6 dogs. The animals of group I were treated with povidone iodine (5.0%) and in group II, III, IV animals were treated with the herbal ointment which were prepared from hydroalcoholic extract of Marigold leaves, Guava leaves and 1:1 mixture of both extract for 7 consecutive days. Clinical and haematological parameters were recorded at different time intervals in all four groups. The study revealed better wound healing properties of the Ointment of 1:1 mixture of Marigold and Guava (10% w/w) leaves extract without any side effect as compared to povidone iodine 5%, ointment of Marigold leaves extract (10% w/w) and ointment of Guava leaves extract (10% w/w) on the basis of clinical and haematological parametersItem Influence of Eugenia jambolana and Psidium guajava Leaf Meal Mixture Supplementation on Carcass Characteristics and Economics of Broiler Chickens(Association of Mastitis, 2020-06) Zargar, M.A.; Pathak, A.K.; Sharma, R.K.; Daing, M.I.Present study was conducted in 120 day-old unsexed broiler chicks. They were randomly distributed into 4 dietary treatments (T1, T2, T3 and T4) supplemented with leaf meal mixture (LMM) of Eugenia jambolana and Psidium guajava@ 0, 2.5, 5.0 and 7.5 % of diet, respectively of 30 birds per treatment, each having 3 replicates (10 chicks per replicate) in a complete randomized block design (CRD). Significantly (P<0.05) higher mean body weight was observed in T1 as compared to T2, T3 and T4 groups. Carcass yields viz. live weight, de-feathered weight, slaughtered weight and eviscerated weights (in gram and % LWt.) were statistically non significant (P>0.05) with and without LMM supplemented diets. However, weights of spleen and liver were significantly (P<0.000, P<0.026) higher in LMM supplemented groups as compared to un-supplemented control (T1). Dietary supplementation significantly reduced abdominal fat content in T4 compared to T1, whereas, T2 and T3 have an intermediate values between T1 and T4 and increased the net profit, it ranged from ` 30.41 to 34.24 per birdItem Isolation, Identification and Antibacterial Activity of Endophytic Bacteria from Leaves of Acacia catechu(Association of Mastitis, 2020-06) Soman, Swathi; Sharma, R.K.; Sharma, Varsha; Gautam, Vidhi; Shrman, K.; Sawarkar, Alka; Kewat, Rituraj; Kumar, ManishDrug resistance in bacteria is becoming an alarming problem in recent years. Post-antibiotic era is not so far. The need for novel antibiotics, chemotherapeutic agents and agrochemicals are utmost. Endophytes are microorganisms that reside in the tissues of living plants which are relatively unstudied and are potential sources of novel natural products for exploitation in medicine, agriculture and industry. The endophytes of medicinal plants participate in biochemical pathways and produce novel bioactive compounds. The aim of the study was to isolate, identify and to assess antibacterial activity of endophytic bacteria from leaves of Acacia catechu. 18 endophytic bacteria had been isolated and identified by sequencing 16S rRNA gene. The isolated endophytic bacteria were identified as Bacillus humi. Considerable in vitro antibacterial activities against a panel of human pathogenic microorganisms (Staphylococcus aureus, Streptococcus pyogenes, Bacillus cereus, Klebsiella pneumoniae, Salmonella Typhimurium and Escherichia coli) were recorded. Among 18 isolates, 15 isolates had shown inhibitory activity against Staphylococcus aureus and Salmonella Typhimurium and 10 isolates had shown inhibitory activity against Klebsiella pneumoniaeItem Landuse impact on soil physical variability and erodibility in North Western subtropics of India(Triveni Enterprises, 2019-07) Abrol, V.; Sharma, R.K.; Sharma, V.; Sharma, P.; Sharma, K.R.; Kumar, A.; Sharma, M.Aim: The study was conducted to determine the impact of landuse on soil physical properties and erodibility. Methodology: Representative soil samples were collected from surface and sub-surface soil depths. Soil physical properties and erodibility indices viz suspension percentage, dispersion ratio, clay/moisture equivalent ratio, erosion ratio, percolation ratio, clay ratio, erosion index and modified clay ratio were determined using standard procedures. Interrelationship among soil properties and erodibility indices were evaluated using Pearson correlation analysis (at 95% and 99% significance level). Results: Among landuse systems, the clay was highest in forest while least in soils under barren landuse. Lowest bulk density and highest value of maximum water holding capacity was found in forest soils. According to susceptibility to erosion, the landuse systems were found in the following order barren lands > cultivated systems (agriculture and horticulture) > forest lands. The ‘r’ values showed positive and highly significant correlations between sand content and bulk density with suspension percentage (SP), clay ratio (CR), modified clay ratio (MCR), dispersion ratio (DR), percolation ratio (PR), erosion ratio (ER), erosion index (EI) while negative and significant correlations of these erodibility indices were found with clay and porosity. Among the erodibility indices SP, DR, PR, ER and EI were positively and significantly correlated with each other. Interpretation: Physical quality of soil was higher in forest compared to other landuse systems. Erodibility of different landuse systems could be arranged in order barren lands > cultivated systems (agriculture and horticulture) > forests. The study was useful for suggesting remedial measures and landuse planning for futureItem Nutrient Composition, Metabolites and Microbial Counts in Litter Material of Broiler Chicken Fed Condensed Tannins Containing Guava Leaf Meal Supplemented Diets: Reduce Environmental Pollution and Alternate Feedstuffs for Ruminants(Association of Mastitis, 2020-06) Saharan, Vivek; Pathak, A.K.; Sharma, Neelesh; Sharma, R.K.; Kumar, HimalayaPresent study was conducted in 240 day-old broiler chicks to assess the effect of condensed tannins (CT) containing guava leaf meal (GLM) supplemented diet on nutrient composition, litter metabolites viz. pH, moisture and ammonia concentration) and microbial counts after completion of feeding trial of 42 days. Chicks were randomly allocated into 4 dietary treatments, with 4 replicates of 15 chicks in each pen in a completely randomized block design (CRD). Chicks were fed basal diets supplemented with GLM @ 1.5, 2.5 and 3.5 percent, in T1, T2 and T3 groups, respectively, while, T0 group fed only basal diet. Litter materials of chicks showed good nutrient profiles and properly sterilized litter material act as alternative feed ingredient for ruminants feed. Supplementation of GLM in the diets of chicks significantly (P<0.05) reduced moisture, pH, NH3 concentration, harmful Eschericia coli and Clostridia spp. counts in the litter materials and reduced environmental pollution. The litter material of GLM supplemented (T1, T2 and T3) chicken showed significantly higher (P<0.05) CP contents as compared to un-supplemented control. It can be concluded that properly sterilized litter material of GLM supplemented chicks act as cheaper alternative feedstuffs for ruminant’s ration and reduce environmental pollution by lowering NH3 concentration, harmful E. coli and Clostridia counts in broiler litter materialItem Organ Weights and Intestinal Morphology of Broiler Chickens Fed Diets Containing Different Supplements(Association of Mastitis, 2020-06) Chauhan, S.S.; Sharma, R.K.; Singh, B.; Shukla, S.K.; Palod, J.; Singh, M.K.The present study was evaluated the effect of dietary inclusion of various feed supplements and their combination on the performance index, glucose and serum cholesterol for 6 weeks. Day-old broiler chicks (n=252) were randomly assigned to seven treatment groups, each with 3 replicates. The first treatment was designated as control (T0) in which no supplement was added to the feed, while in treatments T1; organic mineral mixture (Organomin forte), T2; organic mineral mixture (Vannamin), T3; probiotics (Microguard), T4; enzyme (Brozyme -XPR) and probiotics, T5; emulsifier (Lipigon) were provided through feed. In the T6 group, 3 percent less energy was given through feed. The weight of vital organs viz. heart, liver, gizzard, and spleen were significantly (P<0.05) increased by the feed supplements of which were in the normal range. The gut morphology was also favorably altered due to feed supplementations. This indicates that the organic mineral mixtures, probiotics, enzymes and emulsifiers, and their combinations can be used as a growth promoter in broiler diets and can improve gut health. These products show promising effects as alternatives for antibiotics as pressure to eliminate growth-promoting antibiotic use increases