Browsing by Author "Saini, Abhishek"

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    Comparative Study Of The Serum Tissue Plasminogen Activator(T朠a) Levels In Diabetic Patient With And Without Foot Ulcer.
    (Indian Society for Health and Advanced Research, 2024-01) T P, Elamurugan; Saini, Abhishek
    Background: Diabetic foot ulcer is associated with signi?cant microangiopathy which causes damage to the vascular endothelial cells leading to increased risk of thrombus formation along with impaired process of ?brinolysis. tPA is the most important part of the ?brinolytic system. It has been proven that the level of tPA in the blood re?ects ?brinolytic activity. Hence t-PA levels can be used to identify patients who have microangiopathy and are at risk of developing diabetic foot ulcer To compare the. Objective: concentration levels of serum tissue plasminogen activator (t-PA) levels in Diabetic patients with and without foot ulcer. Materials and methods: This study was conducted in the Department of General Surgery during the period October 2017 to May 2019. Serum Tissue plasminogen activator (tPA) levels in Diabetic patients with and without foot ulcer were assessed and compared. All the demographic parameters studied were also compared with mean tPA and their signi?cance were analyzed. Results: A total of 85 patients with Diabetic foot ulcer and 85 Diabetic patients without foot ulcer were recruited in the study. Median tPA levels (ng/ml) in a diabetic patients with and without foot ulcer was 3.7 and 4.6 with a statistical signi?cance (p value=0.005). Mean tPA levels in patients who presented with and without gangrene among ulcer group was 3.17�05 and 4.52�69 respectively(p value<0.001). Mean tPA levels in ulcer group who had Wagner's grade of ?2 and >2 were 5.87�79 and 3.51�09 respectively. (p value<0.001). Tissue plasminogen activator (t-PA) concentration is signi?cantly lowerConclusions: in diabetic patients with foot ulcer. Lower concentration of t-PA is associated with microangiopathy complications like foot gangrene and higher Wagner's grade
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    The utility of the Ion Torrent PGM next generation sequencing for analysis of the most commonly mutated genes among patients with colorectal cancer in India
    (Wolters Kluwer - Medknow, 2022-06) Mehra, Simmi; Tiwari, Aseem Kumar; Mehta, Swati Pabbi; Sachdev, Ritesh; Rajvanshi, Chhavi; Chauhan, Rajni; Saini, Abhishek; Vaid, Ashok
    Background: The requirement for the mutation analysis for Kirsten rat sarcoma viral oncogene (KRAS) in colorectal cancer (CRC) is rapidly increasing as it is a predictive biomarker and also, its absence signifies response to anti?epidermal growth factor receptor (anti?EGFR) antibody treatment. The aim of our study was to investigate the pathological diagnosis and distribution of KRAS mutations in colorectal cancer with the use of next generation sequencing platform (Ion Torrent). Methods: A total of 56 CRC samples were tested to identify the genetic mutations, especially KRAS using the primers which included ~2800 COSMIC mutations of 50 oncogenes. Ion Torrent personal genome machine (semiconductor?based sequencing) was used for the sequencing and analysis. Along with KRAS, other 49 genes were also studied for COSMIC mutations. Results: KRAS mutation 25 (44.6%) had the highest frequency, followed by TP53 10 (17.9%) and PIK3CA mutation 4 (7.1%). Of all the KRAS mutations identified, mutations in codon 12 were most frequent followed by mutations in codon 13 and 61. The most frequent substitution was glycine to aspartate mutation in codon 12 (p.Gly12Asp) followed by glycine to valine (p.Gly12Val). Combinations of mutations were also studied. Our study revealed that seven cases (12.5%) had both KRAS and TP53 mutations (highest of all the combinations). Conclusion: The analysis of KRAS mutation frequency and its mutational subtype analysis in human CRCs by using semiconductor?based platform in routine clinical practices have been performed in Indian population. The findings were similar to earlier published reports from the Western literature.