Browsing by Author "Maleewong, W"
Now showing 1 - 20 of 32
Results Per Page
Sort Options
Item Affinity purified oval antigen for diagnosis of Opisthorchiasis viverrini.(2001-12-15) Wongsaroj, T; Sakolvaree, Y; Chaicumpa, W; Maleewong, W; Kitikoon, V; Tapchaisri, P; Chongsa-nguan, M; Cross, J HMonoclonal antibodies (MAb) were raised against an oval antigen of the liver fluke Opisthorchis viverrini which is the causative agent of a parasitosis, i.e. opisthorchiasis in Thailand. The antibodies were used in an affinity column to purify the O. viverrini oval antigen from a crude extract of adult parasites by chromatography. The oval antigen was then used in a membrane (dot) ELISA for detecting antibodies in serum samples of parasitologically confirmed Opisthorchis viverrini infected individuals (adult parasites were found in stools after praziquantel treatment and salt purgation), as well as of individuals infected with other parasites and parasite-free controls. The MAb-based dot-ELISA using the affinity purified O. viverrini oval antigen revealed 100% sensitivity, specificity and accuracy for detecting O. viverrini infection. The test is simple, rapid and highly reproducible. Several samples can be tested at the same time without the requirement for special equipment or much increase in testing time; thus it is suitable for mass screening for O. viverrini exposure, especially in new endemic areas. Furthermore using serum specimens could increase patient and community compliance compared to the conventional parasitological survey which uses stool samples for the detection of O. viverrini ova, without treatment and subsequent salt purgation, this conventional method shows a low sensitivity and is also unpleasant to both the sample donors and the laboratory technicians which has historically shown a further negative impact on the final outcome.Item Analysis of antibody levels before and after praziquantel treatment in human paragonimiasis heterotremus.(1992-06-01) Maleewong, W; Wongkham, C; Pariyanonda, S; Intapan, PEnzyme immunoassays (ELISA) and Western blot analysis were used to determine IgG antibody levels in patients infected with Paragonimus heterotremus from Thailand before and after treatment with praziquantel. An IgG antibody ELISA showed that a substantial reduction of antibody levels occurred after one year of treatment. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot analysis showed that P. heterotremus adult extract is highly complex, consisting of more than 9 antigenic bands with molecular size ranging from 123 kDa to less than 12.3 kDa. Two prominent bands of 31.5 kDa and 18.5 kDa were found to show consistent reactions with all serum samples from the pretreatment group. There was a marked reduction in the intensity of the reaction of the 31.5 kDa band with each serum sample from post-treatment patients but the other bands disappeared during the one year interval.Item Anatomical localization of Gnathostoma spinigerum larval antigens by an indirect fulorescent antibody test.(1989-06-01) Morakote, N; Nateewatana, N; Maleewong, W; Uthong, TIndirect fluorescent antibody test (IFAT) was performed on sections of Gnathostoma spinigerum advanced third-stage larva with gnathostomiasis, angiostrongyliasis, trichinosis, strongyloidiasis and cysticercosis sera. Positive fluorescence was observed with the first three sera. Fluorescence was associated with the anterior part of the esophagus, surface of the cuticle and cytoplasmic granules of the intestine. Absorption of sera with gnathostome antigen did not elicit fluorescence. The results suggest that substances secreted from the esophagus and intestine constitute antigens in excretory-secretory products of the larva.Item Antigenic components of Gnathostoma spinigerum recognized by infected human sera by two-dimensional polyacrylamide gel electrophoresis and immunoblotting.(2000-03-28) Wongkham, C; Maleewong, W; Ieamviteevanich, K; Intapan, P M; Morakote, NAntigenic components of Gnathostoma spinigerum larval extract were revealed by two-dimensional gel electrophoresis (2-DE) and immunoblot analysis using sera from patients with 6 proven cases of gnathostomiasis, 5 presumptive cases of gnathostomiasis, 3 proven cases of angiostrongyliasis, 3 proven cases of cysticercosis, and pooled sera from healthy adults. By the 2-DE, the larval extract was highly complex and consisted of more than 75 polypeptides. Immunoblotting analysis of this larval extract after reaction with each of 6 proven gnathostomiasis sera revealed various numbers of antigenic spots ranging from 30 to 70 spots at the approximate molecular masses of less than 14.4 to more than 94 kDa with isoelectric points (pI) of less than 4.65 to 9.6. Antigenic spots at the approximate molecular mass of more than 30 kDa were recognized with the proven angiostrongyliasis, proven cysticercosis and healthy control sera but these sera did not react with the spots at approximate molecular masses of 23-25 kDa with pI of 8.3-8.5. The reacted spots, which consisted of at least 1 to 2 spots, were unique for the recognition of gnathostomiasis sera. Five out of 6 (83.3%) proven and 4 out of 5 (80%) presumptive gnathostomiasis sera reacted with these specific spots.Item Antigenic components of somatic extract from adult Fasciola gigantica recognized by infected human sera.(1997-12-14) Maleewong, W; Intapan, P M; Tomanakarn, K; Wongkham, CThe antigenic components of Fasciola gigantica somatic extract were revealed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting technique using sera from patients with F. gigantica infection, patients with clinically diagnosed fascioliasis, patients with other infections/illness and healthy adults. By SDS-PAGE, it was found that the somatic product comprised more than 22 polypeptides. Immunoblotting analysis revealed at least 13 components which were strongly recognized by sera of patients with fascioliasis. These antigenic components had molecular weights ranging from less than 14.4 to more than 94 kDa. One antigenic component, i.e. 38 kDa was found to give a consistent reaction with sera of patients with fascioliasis (100% sensitivity and 96.7% specificity). The finding suggests that the 38 kDa components may be a potential diagnostic antigen for fascioliasis.Item Comparison of adult somatic and excretory-secretory antigens in enzyme-linked immunosorbent assay for serodiagnosis of human infection with Fasciola gigantica.(1996-09-01) Maleewong, W; Intapan, P M; Wongkham, C; Tomanakan, K; Daenseekaew, W; Sukeepaisarnjaroen, WAdult somatic antigen extract of Fasciola gigantica was compared with excretory-secretory (ES) antigen in an enzyme-linked immunosorbent assay (ELISA) for serodiagnosis of human fascioliasis gigantica. The absorbance values in ELISA using the adult somatic antigen were not significantly different from the values obtaining using ES antigen (p > 0.05). The diagnostic sensitivity, specificity and positive and negative predictive values of the test using adult somatic extract as antigen were 100%, 98%, 70% and 100%, respectively. On the other hand, these values of the test using adult ES antigen were 100%, 99.3%, 87.5% and 100%, respectively. It appears that both somatic and ES antigens are effective antigens for use in the serodiagnosis of human fascioliasis gigantica.Item Control of Opisthorchis viverrini cercariae using the copepod Mesocyclops leuckarti.(1992-06-01) Intapan, P; Kaewkes, S; Maleewong, WItem A dot-ELISA test using monoclonal antibody-purified antigens for the diagnosis of paragonimiasis caused by Paragonimus heterotremus.(1997-09-30) Maleewong, W; Intapan, P M; Wongkham, C; Pajongthanasaris, M; Morakote, N; Tapchaisri, P; Chaicumpa, WA dot enzyme-linked immunosorbent assay (dot-ELISA) using antigens purified by monoclonal antibody-affinity chromatography was developed for detecting antibodies to Paragonimus heterotremus in four groups of subjects. They consisted of 30 patients with P. heterotremus infection, 93 patients with other parasitic infections, 18 patients with pulmonary tuberculosis and 30 normal, healthy controls. Sensitivity, specificity, as well as positive and negative predictive values of the test were 100, 97, 88, and 100%, respectively.Item Excretory-secretory antigenic components of adult Fasciola gigantica recognized by infected human sera.(1998-09-07) Intapan, P M; Maleewong, W; Wongkham, C; Tomanakarn, K; Ieamviteevanich, K; Pipitgool, V; Sukolapong, VThe immunogenic components of Fasciola gigantica excretory-secretory (ES) products were revealed by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting technic using sera from patients with F. gigantica infection, from patients with clinical suspected fascioliasis, from patients with other illness and from healthy adults. By SDS-PAGE, it was found that the ES products comprised more than 6 polypeptides. Immunoblotting analysis revealed 12 components which were strongly recognized by fascioliasis antisera. These antigenic components had a molecular mass ranging from less than 14.4 to 38 kDa. One antigenic band of 27 kDa was found to give a consistent reaction with fascioliasis antisera (100% sensitivity and 98% specificity). The present findings suggest that the 27 kDa components are sensitive and specific for the diagnosis of human F. gigantica infection.Item Gnathostoma spinigerum: analysis of protein patterns by two dimensional gel electrophoresis.(2000-03-07) Wongkham, C; Maleewong, W; Ieamviteevanich, K; Intapan, P M; Morakote, NThe protein extracts from male (MS) and female (FS) adults and advanced third-stage larvae (LS) of Gnathostoma spinigerum were separated by high resolution two-dimensional gel electrophoresis (2-DE). The polypeptide spots, as detected by silver staining, were subsequently identified. The spot patterns of LS, MS and FS were highly complex and consisted of more than 75, 44, 52 prominent spots, respectively. In addition, the stage-specific protein patterns were identified. This 2-DE database should provide an important reference for future biological and biochemical studies of G. spinigerum.Item Immunoblot evaluation of the specificity of the 29-kDa antigen from young adult female worms Angiostrongylus cantonensis for immunodiagnosis of human angiostrongyliasis.(2001-12-15) Maleewong, W; Sombatsawat, P; Intapan, P M; Wongkham, C; Chotmongkol, VThe antigenic components of Angiostrongylus cantonensis young adult female worm somatic extract (FSE) were revealed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting. The sera tested were from patients with proven angiostrongyliasis, other parasitic diseases, and healthy adults. Both the sera and cerebrospinal fluid (CSF) were tested from patients with clinical angiostrongyliasis. The CSF from patients with other neurological diseases were also included. Using SDS-PAGE, we found that the FSE comprised more than 30 polypeptides. Immunoblot analysis revealed at least 12 or 13 antigenic bands in patients with proven or clinical angiostrongyliasis, respectively. The patterns of reactivity recognized by the serum and CSF antibodies against FSE were similar. These antigenic components had molecular masses ranging from less than 14.4 to more than 94 kDa. The prominent antigenic band of 29-kDa might serve as a reliable marker for the diagnosis of angiostrongyliasis. The sensitivity, specificity, positive and negative predictive values of immunoblot analysis in this antigenic band were 55.6%, 99.4%, 83.3% and 97.4%, respectively.Item Laboratory evaluation of Bacillus thuringiensis H-14 against Aedes aegypti larvae in the northeast region of Thailand.(1991-09-01) Pipitgool, V; Maleewong, W; Daenseegaew, W; Thaiklar, KLaboratory bioassays using a preparation of Bacillus thuringiensis H-14 (Bt.H-14), namely Skeetal were conducted to determine their effectiveness against late 3rd/early 4th instar larvae of Aedes aegypti. The larvae were collected from municipal areas in 7 provinces, namely Burirum, Roi-Et, Khon Kaen, Ubol Ratchatani, Nakorn Phanom, Surin and Nakorn Ratchasima, in the Northeast of Thailand. It was found that for Skeetal, LC50 ranged from 128 to 151 nl/l (average 143) and LC90 ranged from 254 to 289 nl/l (average 275). The mortality rate of Ae. aegypti larvae in the 7 provinces did not differ significantly (p greater than 0.05) at a concentration of 300 nl/l. The result of the bioassays show that the preparation of Bt.H-14 is very effective against Ae. aegypti larvae in Northeast of Thailand and the mosquito larvae in the various areas were nearly equal in susceptibility to Bt.H-14.Item Mammonogamus (Syngamus) laryngeus infection: a first case report in Thailand.(1992-06-01) Pipitgool, V; Chaisiri, K; Visetsupakarn, P; Srigan, V; Maleewong, WItem Partially purified antigens of Paragonimus heterotremus for serodiagnosis of human paragonimiasis.(1994-03-01) Wongkham, C; Maleewong, W; Intapan, P; Morakote, N; Chaicumpa, WThe preparative crude extract of Paragonimus heterotremus was fractionated by isoelectric focusing. Fractions at pH 5 which contained a specific antigen with a relative molecular weight of 31.5 kDa were pooled and used in an indirect enzyme-linked immunosorbent assay (ELISA) and immunoblot analysis for diagnosis of human paragonimiasis. The sensitivity and specificity of ELISA were found to be 100% and 99% respectively. The band of 31.5 kDa antigenic component was found to give consistent reaction with paragonimiasis sera. The sensitivity, specificity and predictive value (positive and negative) of immunoblot analysis for the 31.5 kDa band were all 100%.Item Prevalence and intensity of Opisthorchis viverrini in rural community near the Mekong River on the Thai-Laos border in northeast Thailand.(1992-04-01) Maleewong, W; Intapan, P; Wongwajana, S; Sitthithaworn, P; Pipitgool, V; Wongkham, C; Daenseegaew, WThe prevalence and intensity of Opisthorchis viverrini in fourteen villages in Nakhon-Phanom province, Northeast, Thailand have been investigated. Overall prevalence of O. viverrini infection was 66.4 per cent in a total population of 2,412 individuals. The prevalence was 18.5 per cent in children under 5 years, 38.9 per cent in those aged 5-9 years, and ranged from 64.9 per cent to 82.2 per cent in the age group above 10 years. The intensity of O. viverrini infection increased with age. The mean faecal egg output was highest in the 30-34 year age group and remained relatively constant through older ages. In all age groups the prevalence and intensity of infection in both men and women were similar. The population was divided according to the presence and intensity of infection as follow, 33 per cent were uninfected, 59 per cent had light infections (less than 1,000 eggs per g of faeces; EPG), 7 per cent had moderate infections (1,000-10,000 EPG), and 1 per cent had heavy (greater than 10,000 EPG). Other important intestinal infections found in this community are hookworm, Taenia spp. and Trichuris trichiura with the prevalence of 17.9 per cent, 1.1 per cent and 1.1 per cent respectively.Item Prevalence of toxoplasma antibodies in blood donors and pregnant women in Khon Kaen Province.(1989-05-01) Maleewong, W; Lulitanond, V; Pipitgool, V; Auwijitaroon, Y; Kuttsarejariya, S; Morakote, NSerum samples from blood donors and pregnant women in Khon Kaen were examined for antibodies to Toxoplasma by an indirect hemagglutination and indirect fluorescent antibody techniques. It was found that 6.4 per cent of the blood donors were positive by the indirect hemagglutination and 6.2 per cent by indirect fluorescent antibody tests. The seroprevalence in pregnant women were 12.0 per cent by indirect hemagglutination and 4.7 per cent by indirect fluorescent antibody tests. The frequency distribution curves of indirect hemagglutination titers were unimodal in both the groups studied. From the basis of these findings, it was concluded that toxoplasmosis is not endemic in Khon Kaen and the transmission occurs at a very low level.Item Recent advances in diagnosis of paragonimiasis.(1997-03-01) Maleewong, WParagonimiasis in endemic areas can be diagnosed by clinical symptoms. However, the diagnosis should always be confirmed by microscopic examination of the sputum or stool in order to find Paragonimus eggs. Within recent years marked advances in diagnosis of paragonimiasis have been made. Two new approaches comprising a genetic probe and immunological tests have been developed with claims to be as good or better than microscopic examinations. This report reviews these two areas, especially in paragonimiasis caused by Paragonimus heterotremus and P. westermani. In addition, problem areas in assay development are discussed.Item Scanning electron microscopic study of nocturnally subperiodic Brugia malayi (Filarioidea: Onchocercidae), Narathiwat, Southern Thailand.(1987-03-01) Choochote, W; Maleewong, W; Sucharit, S; Pipitgool, V; Khamboonruang, CScanning Electron Microscopic (SEM) observations were made on the adult females and males of nocturnally subperiodic Brugia malayi (Narathiwat, Southern Thailand) from 8-month-old intra-peritoneally infected jirds (Meriones unguiculatus). Descriptions of the morphological surfaces of anterior end, vulva, body cuticle, anus, posterior end of females and anterior end, body cuticle, cloaca, caudal papillae, spicules, sheath, posterior end of males were demonstrated. The comparison among these and other filarial parasites were also investigated.Item Scanning electron microscopic study of pupal seta 9-111-V of Anopheles balabacensis (Perlis Form) and Anopheles dirus (Bangkok strain).(1987-12-01) Choochote, W; Maleewong, W; Sucharit, S; Tesana, SItem Scanning electron microscopic study of third-stage larva of Wuchereria bancrofti and Brugia malayi in Thailand.(1987-06-01) Maleewong, W; Choochote, W; Sukhavat, K; Khamboonruang, C; Arunyanart, C