Partially purified antigens of Paragonimus heterotremus for serodiagnosis of human paragonimiasis.
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Date
1994-03-01
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Abstract
The preparative crude extract of Paragonimus heterotremus was fractionated by isoelectric focusing. Fractions at pH 5 which contained a specific antigen with a relative molecular weight of 31.5 kDa were pooled and used in an indirect enzyme-linked immunosorbent assay (ELISA) and immunoblot analysis for diagnosis of human paragonimiasis. The sensitivity and specificity of ELISA were found to be 100% and 99% respectively. The band of 31.5 kDa antigenic component was found to give consistent reaction with paragonimiasis sera. The sensitivity, specificity and predictive value (positive and negative) of immunoblot analysis for the 31.5 kDa band were all 100%.
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The Southeast Asian Journal of Tropical Medicine and Public Health.
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Citation
Wongkham C, Maleewong W, Intapan P, Morakote N, Chaicumpa W. Partially purified antigens of Paragonimus heterotremus for serodiagnosis of human paragonimiasis. The Southeast Asian Journal of Tropical Medicine and Public Health. 1994 Mar; 25(1): 176-80