Browsing by Author "Goyal, Manu"
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Item CALLA negative precursor B lymphoblastic leukemia with MLL gene translocation and an unusual FISH signal pattern.(2011-01) Devi, Sandhya G; Goyal, Manu; Ramakrishna, N V S S; Murthy, S SudhaRearrangements of the mixed lineage leukemia (MLL) gene at 11q23 commonly occur in infants with CALLA negative B lymphoblastic leukemia (B-ALL). Most often, these are detected by conventional karyotyping; however, fluorescent in-situ hybridization (FISH) with the help of a dual-color break-apart probe is used to identify cryptic translocations. When there is an MLL gene translocations the usual FISH signal pattern is 1 red-1 yellow fusion signal pattern We present a case of an infant with CALLA negative precursor B-ALL with a characteristic translocations t(4;11) (q21;q23),however,with an unusual MLL FISH signal pattern.Item Cryptic PML-RARα positive acute promyelocytic leukemia with unusual morphology and cytogenetics.(2010-10) Goyal, Manu; Dattatreya, Palinki S; Goud, Irawathy; Murthy, Sudha SAcute Promyelocytic Leukemia (APL) is different from other forms of acute myeloid leukemia (AML), to the reason being the potential devastating coagulopathy and the sensitivity to all-trans retinoic acid (ATRA) and arsenic trioxide (As 2 O 3 ). We hereby present a case of APL, morphologically distinct from the hypergranular APL; however, the flow cytometry revealed a characteristic phenotype showing dim CD45, bright CD13, bright CD33 and dim CD117 positivity. These were negative for CD34, HLA-DR, B-lymphoid and T-lymphoid lineage markers. Conventional cytogenetics revealed a distinct karyotype of a male with translocation t(4;15)(q34.2:q26.3). However, interphase florescence-in-situ hybridization (FISH) revealed PML/RARA fusion signal on chromosome 15 in 90% cells. The cryptic translocations may be missed on conventional cytogenetics, however, need to be picked by other techniques as FISH.Item dic(7;9) with loss of Tp53 gene in acute lymphoblastic leukemia.(2016-10) Gupta, Anurag; Reddy, Krishna G; Goyal, Manu; Karwa, Pankaj; Swarupa, Divya G; Kasaragadda, Mukkanteeswara RaoItem Pain relief during minor procedures: a challenge for gynaecologists.(2015-10) Mittal, Pratima; Goyal, ManuItem Your dilemma, my identity: Unusual immunogenetic profiles of pediatric B cell acute lymphoblastic leukemia.(2014-01) Gupta, Anurag; Goyal, Manu; Nidamanuri, Koteswara Rao; Dattatreya, Palanki SatyaB-cell acute lymphoblastic leukemia (B-ALL) is characterized by CD19 expression, which is one of the most important prerequisites, along with expression of CD10, CD22 and/or CD79a. Rearrangements involving MLL gene are seen in CD10− B-ALL (pro-B cell origin) and t(9;11)(p21;q23) is most commonly reported in acute myeloid leukemia (AML), where it is known to carry very good prognosis in pediatric AMLs and rarely in acute lymphoblastic leukemia (ALL). We report a case of CD10+, CD19− pediatric ALL with rearrangements of MLL gene as a result of t(9;11)(p21;q23), thus conferring a very poor prognosis. The case emphasizes use of comprehensive panel of antibodies for fl ow cytometric immunophenotyping and cytogenetic correlation for correct diagnosis and prognostication. KEY WORDS: Acute lymphoblastic leukemia, CD19, MLL gene, t(9;11)(p21;q23)