Browsing by Author "Ghosh, K"

Now showing 1 - 20 of 82
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    Acquired hemostatic defects during L-asparaginase therapy.
    (1986-07-01) Marwaha, N; Marwaha, R K; Sarode, R; Kaur, S; Ghosh, K; Garewal, G; Mohanty, D
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    Acute megakaryoblastic leukaemia--an underdiagnosed entity.
    (1986-06-01) Bidwai-Bhattacharjee, M; Shome, D K; Srinivas, M; Ghosh, K; Verma, S; Mohanty, D; Bhagwat, A G; Das, K C
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    Acute myeloid leukemia in a hemophilic--a serendipitous coexistence.
    (1984-09-01) Shome, D K; Marwaha, N; Marwaha, R K; Ghosh, K; Garewal, G; Mohanty, D
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    Acute renal failure due to lymphomatous infiltration of kidneys (a case report).
    (1981-08-01) Raina, V; Ghosh, K; Malaviya, A N
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    Acute systemic toxic reactions caused by hornet stings.
    (1988-08-01) Ghosh, K; Singh, S; Pereira, B J; Singhi, S C
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    ANCA: serology in Wegener's granulomatosis.
    (2005-07-03) Pradhan, Vandana D; Badakere, S S; Ghosh, K; Almeida, A
    BACKGROUND AND OBJECTIVES: Wegener's granulomatosis (WG) is being increasingly diagnosed in India, which exists in two forms, the 'limited Wegener's granulomatosis' (LWG) having upper respiratory tract (URT) and lower respiratory tract (LRT) involvement and the 'classical Wegener's granulomatosis' (CWG), with the triad of URT, LRT involvement along with kidney involvement. Cytoplasmic ANCA (C-ANCA) or anti-Proteinase3 (anti-PR3), which is highly diagnostic for WG, rarely perinuclear ANCA (P-ANCA) may exist. AIMS: To detect anti-neutrophil cytoplasmic antibodies (ANCA) and correlate it with serological, hematological parameters, and the Birmingham Vasculitis Activity Score (BVAS). SETTINGS AND DESIGN: Twenty-three clinically and histopathologically proven WG (16 CWG, 7 LWG) were studied. MATERIAL AND METHODS: C-ANCA and P-ANCA patterns were identified by immunofluorescence and specificities were confirmed by 'alpha granule' enzyme linked immunosorbent assay (ELISA), anti-PR3, anti-MPO (myeloperoxidase) and anti-Lactoferrin (anti-LF) by ELISA. RESULTS: LRT involvement was seen in 91.3%, URT in 78.3%, and renal manifestations in 69.6% cases. The BVAS in CWG was significantly higher than BVAS in the LWG. Decreased hemoglobin, increased WBC counts, ESR, CRP and Creatinine were seen in CWG as compared to LWG. The C-ANCA was present in 65.2% patients and P-ANCA in 13% cases. Anti-PR3 was seen in 69.6% patients and anti-LF in 17.4% cases. Severity of disease and ANCA was higher in CWG than in LWG. CONCLUSIONS: Vasculitis syndromes are known to overlap and many go undetected; therefore ANCA testing, along with the clinical and histopathological observations may be helpful in early detection and management of WG cases.
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    APO-1/Fas gene: Structural and functional characteristics in systemic lupus erythematosus and other autoimmune diseases.
    (2009-09) Singh, Richa; Pradhan, Vandana; Patwardhan, Manisha; Ghosh, K
    Systemic lupus erythematosus (SLE) is an autoimmune disorder affecting multiple organ systems. It is characterized by the presence of autoantibodies reactive against various self-antigens. Susceptibility to SLE is found to be associated with many major histocompatibility complex (MHC) and non-MHC genes, one of which is APO-1/Fas gene, which is present on chromosome 10 in humans. The APO-1/Fas promoter contains consensus sequences for binding of several transcription factors that affect the intensity of Fas expression in cells. The mutations in the APO-1/Fas promoter are associated with risk and severity in various autoimmune diseases and other malignancies. The APO-1/Fas receptor is expressed by many cell types. Two forms of APO-1/Fas protein that are involved in regulation of apoptosis have been identified. Fas receptor-mediated apoptosis plays a physiological and pathological role in killing of infected cell targets. In this review, we have focused on APO-1/Fas gene structure, promoter variants and its association with SLE and other autoimmune diseases. Functional aspects of Fas receptor in apoptosis are also discussed.
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    Apoptosis.
    (1995-07-01) Ghosh, K
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    Autoantibodies, immunoglobulins, complement and circulating immune complexes in acute malaria.
    (1997-01-01) Jhaveri, K N; Ghosh, K; Mohanty, D; Parmar, B D; Surati, R R; Camoens, H M; Joshi, S H; Iyer, Y S; Desai, A; Badakere, S S
    BACKGROUND: Malaria caused by Plasmodium vivax and Plasmodium falciparum is common in the Indian subcontinent. Studies conducted elsewhere have suggested that malarial infection causes intense immunostimulation. We screened patients with malarial infection for autoantibodies and measured the immunoglobulin, circulating immune complex and complement levels to determine the extent of immunological alterations in these patients. METHODS: One hundred adults with acute malarial infection confirmed by examination of the peripheral blood smear and 25 age- and sex-matched controls were studied. An autoantibody screen and serum immunoglobulin complement (C3 and C4) and circulating immune complex levels were measured at the time of admission and 4 weeks after they became afebrile. A direct Coomb's test was also done. RESULTS: Anti-ssDNA, anti-dsDNA and rheumatoid factor were positive at the time of admission in 51, 30 and 38 patients respectively. None of the controls were positive for these autoantibodies except for one who was positive for rheumatoid factor. The IgM, IgG and IgA levels were raised in 16, 25 and 36 patients respectively. Circulating immune complex levels were raised in 32 patients and complement C3 and C4 were low in 8 and 31 patients. Follow up studies at 4 weeks in 19 patients showed that the autoantibodies were negative. However, the immunoglobulin, C4 and circulating immune complex levels remained elevated. Six per cent of patients had a positive direct Coomb's test with reticulocytosis at the time of presentation. CONCLUSION: Acute malarial infection can cause false-positive results for anti-ssDNA, anti-dsDNA and rheumatoid factor and may also cause a rise in the serum immunoglobulin, complement and circulating immune complex levels.
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    Carrier detection and prenatal diagnosis in families with haemophilia.
    (2001-03-09) Shetty, S; Ghosh, K; Bhide, A; Mohanty, D
    BACKGROUND: Haemophilias are the commonest X-linked disorders affecting approximately 1 in 10,000 male births. Detection of carrier women in families with haemophilia and subsequent antenatal diagnosis of confirmed carriers are important services for these patients and their relatives. Over the last 6 years we performed carrier detection and antenatal diagnosis in families with patients of haemophilia A and B. METHODS: During the last 6 years, 159 families with haemophilia A and B were analysed for carrier detection by DNA analysis, using various polymorphic markers of factors VIII and IX genes. The polymorphisms used were intron 18 Bcl I, intron 19 Hind III, intron 22 Xbal and DXS52/St14 of the factor VIII gene and intron I Ddel, intron 4 Taql, 3 Hhal and Residue 148 codon Mnll of the factor IX gene. There were 189 probable carriers (whose carrier status was not known) and 99 obligatory carriers (confirmed carriers by family pedigree analysis) from 102 families with haemophilia A. Of the 57 families with haemophilia B analysed, there were 98 probable and 52 obligatory carriers. All the analyses were carried out by polymerase chain reaction. For antenatal diagnosis, prior to polymorphism analysis, the sex of the foetus was detected by Y chromosome-specific amplification. RESULTS: One hundred and four females were diagnosed as carriers and 63 as non-carriers by the intragenic polymorphic markers in families with haemophilia A. Eighteen women were informative with only the extragenic marker of factor VIII gene. Four women were not informative with any of the markers used. In families with haemophilia B, 37 women were diagnosed as carriers and 34 as non-carriers by the intragenic markers and 34 were informative only with the extragenic markers. Seventeen women were not informative with any of the markers used. Of the 25 antenatal diagnoses performed (20 haemophilia A, 5 haemophilia B) using the same markers as those used in carrier detection, 14 were male foetuses and 11 female as detected by Y chromosome-specific polymerase chain reaction. Eight were affected males and 6 unaffected. Among the females, 5 were carriers and 6 normal. CONCLUSION: Using the above polymorphic markers of factors VIII and IX genes, a diagnosis could be made in the majority of families.
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    Cellular composition and reticulin fibrosis in chronic myeloid leukaemia.
    (1988-09-01) Ghosh, K; Varma, N; Varma, S; Dash, S
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    Changes in histone pattern of mouse skin epidermis after single application of 20-methylcholanthrene.
    (1983-01-01) Ghosh, K; Das, S; Chatterjee, R; Chowdhury, J R
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    Chromosomal variants and genetic diseases.
    (2005-05) Rao, V Babu; Ghosh, K
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    Clinical and Immunological Profile of Systemic Lupus Erythematosus.
    (2013-04) Pradhan, V; Patwardhan, M; Rajadhyaksha, A; Ghosh, K
    Pediatric onset systemic lupus erythematosus (SLE) is not uncommon and female to male ratio varies. Pediatric SLE patients have more severe disease at onset, higher rates of organ involvement and more aggressive clinical course than adults. We compared the clinical and immunological parameters among pediatric SLE and adult SLE from Western India. Twenty five children and 60 adult patients fulfilling American College of Rheumatology SLE criteria were included. Anti-nuclear antibodies, anti-dsDNA and complement (C3, C4) levels were tested. Of 25 pediatric SLE patients studied, 24% showed CNS involvement vs. 8.3% in adults SLE (P=0.0499). Lupus nephritis was seen in 75% adult patients vs. 52% among children. Hepatosplenomegaly was noted more among adult SLE 26.8% vs 12% among children. Alopecia was an exclusive features among adult SLE.
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    Clinical approach to breast disorders: A primer for internists.
    (2006-05-17) Ghosh, K; Ghosh, A K
    Women with breast disorders often present first to their internists for evaluation. A delay in accurate diagnosis could often result in worsening of prognosis for the patient. Common diagnostic problems include evaluation of a breast lump, nipple discharge and breast pain. Internist now has a choice of several diagnostic modalities to evaluate breast disorders. An evidence-based approach to common breast symptoms and advances in breast diagnostic techniques has been described to enable early diagnosis of breast cancer, thereby reducing the need for intensive treatments and improve patient satisfaction and clinical outcome.
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    Clinical examination and hematological data in asymptomatic & apparently healthy school children in a boarding school in a tribal area.
    (2002-04-26) Ghosh, K; Mukherjee, M B; Shankar, U; Kote, S L; Nagtilak, S B; Kolah, R B; Surve, R R; Tamankar, A A; Sukumar, S; Mohanty,
    In a boarding school of Maharashtra State of India 314 students (Bhil & Pawar) were examined clinically and blood was examined. Anemia was present in 16.2% male & 38.3% female. B (Beta). Thalasemia trait was present in 1.6% male & 2.4% female. Sickle cell trait was present in 21.3% male and 14.4% female and sickle cell disease in 0.6% student. G6PD deficiency was seen in 5.1% male & 4.8% female students.
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    Combined Down and Klinefelter syndrome.
    (2003-09-08) Babu Rao, V; Ghosh, K
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    Combined staining for esterases in acute non-lymphoblastic leukaemia.
    (1986-08-01) Shome, D K; Ghosh, K; Mohanty, D
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    Comparative case control study of clinical features and human leukocyte antigen susceptibility between familial and nonfamilial vitiligo.
    (2009-11) Misri, Rachita; Khopkar, Uday; Shankarkumar, U; Ghosh, K
    Background: Various studies worldwide suggest that human leukocyte antigen (HLA) region may be involved in the genetic susceptibility of vitiligo but little information is available from India. Aim: To find the HLA associated susceptibility to develop vitiligo in Indian patients and to detect role of HLA in familial vitiligo. Methods: This was a case controlled study which included all patients suffering from vitiligo over a period of one and half years. Clinical details were noted and sera collected from these patients were screened for the presence of HLA class I antibodies. The clinical features and HLA antigens were assessed and comparison was made between patients with familial and nonfamilial vitiligo. Results: Out of 114 patients studied, 84 had family history and 30 had no family history. Patients with family history of vitiligo have higher chances of acquiring vitiligo if first degree relatives are affected compared to if second degree relatives are affected. Family history of vitiligo is associated with an early onset of vitiligo (<20 years). There was no statistically significant difference in the type, stability, and severity of vitiligo in both the groups. HLA results in both the groups revealed increase in HLA A2, A11, A31, A33, B17, B35, B40, and B44 alleles while HLA A9, B13, and B53 alleles were decreased. Family history was associated with HLA A2, A28, A31, and B44 alleles. Early onset of vitiligo (<20 years) was significantly associated with HLA A2, A11, B17, B35, and B44 alleles. The patients with severe affection (>10% area) showed in significant association with HLA A10 and B8. Conclusion: Family history of vitiligo is associated with an early onset of vitiligo. There is no correlation of family history with the type of vitiligo, stability of lesions, and areas involved. Severity is not associated with family history. Apart from other alleles, alleles A2, and B44 play a significant role in vitiligo in the Indian patients.