Indian Journal of Experimental Biology

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Browsing Indian Journal of Experimental Biology by Author "Abaci, Neslihan"

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    Effect of Nigella sativa L. extract and thymoquinone on the genes responsible for cell proliferation, migration and NK cell cytotoxicity in breast cancer
    (The India Publications and Information Directorate (CSIR-NISCAIR), 2024-05) Korak, Tu?can; Emrence, Zeliha; Ekmekci, Sema S?rma; Abaci, Neslihan; Ergül, Emel
    The black cumin, Nigella sativa L. is known to be effective against various diseases including cancer. Thymoquinone (TQ), active ingredient of N. sativa extracts, can inhibit proliferation metastasis and regulating immune system in different cancers as with breast cancer (BC). The mechanisms of action behind TQ are not clearly understood yet. The purpose of the current study is to investigate the effects of TQ, water (WE) and alcohol extracts (AE) of N. sativa on BC cells by focusing attention on the following genes; CDK4, MYC, NF-?B1, VEGFA, FGF1, N-cadherin, ULBP1, ULBP2 and CD155. Conventional protocols were performed in order to obtain extracts. Cell viability was measured by RTCA and MTT assay, and gene expressions were analyzed by qRT-PCR. Association was significant for CDK4 (P =0.07), MYC (P <0.001), NF-?B1 (P =0.011), VEGFA (P =0.013), FGF1 (P <0.001), and ULBP1 (P =0.021) genes. CDK4 and MYC genes may be candidate genes for mechanisms involved in reduced cell proliferation induced by AE and TQ. Increased ULBP1 expression through AE and TQ indicates that N. sativa may trigger ULBP1-mediated NK cell cytotoxicity. Our results support the idea that active ingredients in N. sativa promise an encouraging therapeutic approach in the future.