In vitro estrogenic activities of fenugreek Trigonella foenum graecum seeds.

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dc.contributor.authorSreeja, S
dc.contributor.authorAnju, V S
dc.contributor.authorSreeja, S
dc.date.accessioned2011-11-29T09:11:13Z
dc.date.available2011-11-29T09:11:13Z
dc.date.issued2010-06
dc.description.abstractBackground & objectives: Trigonella foenum graecum commonly known as fenugreek, has been widely cultivated in Asia, Africa and Mediterranean countries for the edible and medicinal values of its seeds. Earlier reports show that fenugreek seeds provide a mastogenic effect resulting in enhanced breast size. However, very little is known about its estrogenic effect. The present study investigated the effect of chloroform extracts of fenugreek seeds (FCE) in breast cancer cells for its estrogenic effect, and to assess its capacity as an alternative to hormone replacement therapy (HRT). Methods: The effect of FCE on cell proliferation of estrogen receptor (ER) positive breast cancer cells, MCF-7 was studied by MTT assay at a concentration range of 20 to 320 μg/ml. The competitive ER binding assay (HAP assay) was done to find out the ER binding capacity of the extract. Transfection and reporter assay (DLR assay), and RT- PCR with an estrogen responsive gene pS2 were done to find out the transcriptional regulatory activity of FCE. Results: FCE stimulated the proliferation of MCF-7 cells, showed binding to ER (IC50 = 185.6 ± 32.8 μg/ml) and acted as an agonist for ER mediated transcription via ERE. It also induced the expression of estrogen responsive gene pS2 in MCF-7 cells. Interpretation & conclusion: Our study provided the evidence for estrogenic activities of fenugreek seeds. Further in vitro and in vivo studies could demonstrate its suitability as an alternative to HRT.en_US
dc.identifier.citationSreeja S, Anju V S, Sreeja S. In vitro estrogenic activities of fenugreek Trigonella foenum graecum seeds. Indian Journal of Medical Research. 2010 Jun; 131(6): 814-819.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/135524
dc.language.isoenen_US
dc.source.urihttps://icmr.nic.in/ijmr/2010/june/0613.pdfen_US
dc.subjectEstrogenen_US
dc.subjectestrogen receptoren_US
dc.subjecthormone replacement therapyen_US
dc.subjectphytoestrogenen_US
dc.subjectTrigonella foenum graecumen_US
dc.subject.meshAntineoplastic Agents/pharmacology
dc.subject.meshAntineoplastic Agents --therapeutic use
dc.subject.meshBreast Neoplasms --drug therapy
dc.subject.meshBreast Neoplasms --pathology
dc.subject.meshBreast Neoplasms --physiopathology
dc.subject.meshCell Proliferation --drug effects
dc.subject.meshFemale
dc.subject.meshHumans
dc.subject.meshPhytoestrogens --pharmacology
dc.subject.meshPlant Extracts --pharmacology
dc.subject.meshPlant Extracts --therapeutic use
dc.subject.meshReceptors, Estrogen --genetics
dc.subject.meshReceptors, Estrogen --metabolism
dc.subject.meshSeeds --chemistry
dc.subject.meshTrigonella --chemistry
dc.subject.meshTumor Cells, Cultured --drug effects
dc.titleIn vitro estrogenic activities of fenugreek Trigonella foenum graecum seeds.en_US
dc.typeArticleen_US
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