Evaluation of Nested Polymerase Chain Reaction for the Diagnosis of Pulmonary Tuberculosis.
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Date
2014-07
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Abstract
Utility of Polymerase Chain Reaction (PCR) test for
diagnosis of pulmonary tuberculosis was evaluated in 50
clinically diagnosed cases. All samples were also processed
for microscopy by Ziehl-Neelsen (ZN) staining and culture
on Lowenstein-Jensen medium (LJ). PCR was positive in
all microscopy and culture positive specimens. There were
11 samples positive by PCR but failed to grow in culture.
With respect to culture the sensitivity (SN), specificity (SP),
Positive Predictive Value (PPV) and Negative Predictive
Value (NPV) of PCR was 100%, 57%, 68% and 100%
respectively. The values for PCR on final evaluation taking
into consideration clinical, radiological, microbiological
evidence and response to antitubercular treatment were SN-
100%, SP-88%, PPV-94% & NPV-100% respectively. PCR
detects M. tuberculosis complex with greater sensitivity
and should be useful for rapid diagnosis of tuberculosis.
Description
Keywords
mycobacterium tuberculosis, polymerase chain reaction, IS 6110, sensitivity, specificity
Citation
Bansal Vishvesh P, Damale Ajit S, Patwardhan Neeta S, Bansal Mangala P. Evaluation of Nested Polymerase Chain Reaction for the Diagnosis of Pulmonary Tuberculosis. Indian Medical Gazette. 2014 Jul ; 148 (7): 259-264.