Preliminary comparative analysis of antibacterial effects of activated and non-activated of expired platelet concentrate by disc diffusion method.
| dc.contributor.author | Zabidi, Muhammad Azrul | |
| dc.contributor.author | Yusoff, Narazah Mohd | |
| dc.contributor.author | Kader, Zainoodin Sheik Abdul | |
| dc.date.accessioned | 2012-10-16T04:30:37Z | |
| dc.date.available | 2012-10-16T04:30:37Z | |
| dc.date.issued | 2012-01 | |
| dc.description.abstract | Background: Platelets release more than 30 cytokines to provide primary hemostatic function. In addition, platelets are also known to release antimicrobial peptides upon activation by thrombin. Materials and Methods: In this study, comparative analysis of antibacterial activity of activated and non-activated expired platelet concentrate was determined against Gram-positive and Gram-negative bacteria by Kirby-Bauer disk diffusion method. Thrombin was used to prepare activated platelet concentrate. Gram-positive bacteria tested in this study were S.aureus and S.pyogenes and Gram-negative bacteria were E.coli and K.oxytoca. All the bacteria used in this study were sensitive strains from clinical isolates. Activated and non-activated platelet showed no zone of inhibition against S.pyogenes and E.coli. Results: Activated platelet showed antibacterial activity against S.aureus and K.oxytoca with the zone of inhibition of 8.3 ± 0.6 mm and 7.7 ± 0.2 mm, respectively. Zone of inhibition observed in non-activated platelet against S.aureus and K.oxytoca were 7.8 ± 0.4 mm and 7.5 ± 0.3 mm, respectively. Conclusions: These findings showed that no significant differences in antibacterial activity produced by activated and non-activated platelet. However, zone of inhibition observed in activated and non-activated platelet indicate the presence of antibacterial property in expired platelet. | en_US |
| dc.identifier.citation | Zabidi Muhammad Azrul, Yusoff Narazah Mohd, Kader Zainoodin Sheik Abdul. Preliminary comparative analysis of antibacterial effects of activated and non-activated of expired platelet concentrate by disc diffusion method. Indian Journal of Pathology & Microbiology. 2012 Jan-Mar 55(1): 47-51. | en_US |
| dc.identifier.uri | https://imsear.searo.who.int/handle/123456789/142175 | |
| dc.language.iso | en | en_US |
| dc.source.uri | https://www.ijpmonline.org/article.asp?issn=0377-4929;year=2012;volume=55;issue=1;spage=47;epage=51;aulast=Zabidi | en_US |
| dc.subject | Disc diffusion assay | en_US |
| dc.subject | expired platelet concentrate | en_US |
| dc.subject | platelet microbicidal peptide | en_US |
| dc.subject.mesh | Antimicrobial Cationic Peptides --metabolism | |
| dc.subject.mesh | Antimicrobial Cationic Peptides --pharmacology | |
| dc.subject.mesh | Blood Platelets --chemistry | |
| dc.subject.mesh | Gram-Negative Bacteria --drug effects | |
| dc.subject.mesh | Gram-Positive Bacteria --drug effects | |
| dc.subject.mesh | Humans | |
| dc.subject.mesh | Microbial Sensitivity Tests | |
| dc.title | Preliminary comparative analysis of antibacterial effects of activated and non-activated of expired platelet concentrate by disc diffusion method. | en_US |
| dc.type | Article | en_US |