Detection of Epstein-Barr virus DNA and HHV-6 DNA in tissue biopsies from patients with nasopharyngeal carcinoma by polymerase chain reaction.

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dc.contributor.authorKositanont, Uen_US
dc.contributor.authorKondo, Ken_US
dc.contributor.authorChongkolwatana, Cen_US
dc.contributor.authorMetheetrairut, Cen_US
dc.contributor.authorPuthavathana, Pen_US
dc.contributor.authorChantarakul, Nen_US
dc.contributor.authorWasi, Cen_US
dc.contributor.authorYamanishi, Ken_US
dc.date.accessioned2009-05-27T14:39:23Z
dc.date.available2009-05-27T14:39:23Z
dc.date.issued1993-09-01en_US
dc.descriptionThe Southeast Asian Journal of Tropical Medicine and Public Health.en_US
dc.description.abstractA total of 34 tissue biopsies were collected from nasopharyngeal carcinoma (NPC) patients and 5 controls with non-NPC. Extracted DNA from tissue biopsies were analyzed for presence of specific gene sequences to EBV type A and type B, and HHV-6 by polymerase chain reaction (PCR). The different sequences of EBV type A and B were parts from the highly divergent forms of the EBV nuclear antigen 2 (EBNA 2). The PCR amplified products for EBNA 2A and EBNA 2B were 115 and 119 base pairs respectively whereas that of HHV-6 DNA was 776 base pairs. The results demonstrated that EBV DNA was detected in 32 of 34 cases (94.1%): 28 (82.3%) with type A, 2 (5.9%) with type B, and 2 (5.9%) with both types. EBV DNA of type A could be detected 1 (20%) of 5 controls. HHV-6 DNA was in 5 of 34 samples (14.7%) whereas HHV-6 DNA was not detectable in biopsy tissues from controls. The results show that in the NPC patient group, A type of EBV is predominant. Detection of HHV-6 DNA in patients group only might be resulted from reactivation of a latent infection or association with EBV-induction of NPC.en_US
dc.description.affiliationDepartment of Microbiology, Faculty of Medicine Siriraj Hospital, Bangkok, Thailand.en_US
dc.identifier.citationKositanont U, Kondo K, Chongkolwatana C, Metheetrairut C, Puthavathana P, Chantarakul N, Wasi C, Yamanishi K. Detection of Epstein-Barr virus DNA and HHV-6 DNA in tissue biopsies from patients with nasopharyngeal carcinoma by polymerase chain reaction. The Southeast Asian Journal of Tropical Medicine and Public Health. 1993 Sep; 24(3): 455-60en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/30722
dc.language.isoengen_US
dc.source.urihttps://www.tm.mahidol.ac.th/seameo/publication.htmen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshBiopsyen_US
dc.subject.meshBlotting, Southernen_US
dc.subject.meshCarcinoma --classificationen_US
dc.subject.meshCase-Control Studiesen_US
dc.subject.meshDNA, Viral --analysisen_US
dc.subject.meshElectrophoresis, Agar Gelen_US
dc.subject.meshHerpesvirus 4, Human --classificationen_US
dc.subject.meshHerpesvirus 6, Human --geneticsen_US
dc.subject.meshHumansen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshNasopharyngeal Neoplasms --classificationen_US
dc.subject.meshPolymerase Chain Reaction --methodsen_US
dc.titleDetection of Epstein-Barr virus DNA and HHV-6 DNA in tissue biopsies from patients with nasopharyngeal carcinoma by polymerase chain reaction.en_US
dc.typeJournal Articleen_US
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