Association between the organic cation transporter 3 methylation and hepatocellular carcinoma
Loading...
Date
2023-03
Journal Title
Journal ISSN
Volume Title
Publisher
CSIR-NIScPR
Abstract
Hepatocellular carcinoma (HCC) is one of the deadliest cancers in the world and has a high death rate in the world. This research while examining the expression of OCT3 at the mRNA level has also studied gene methylation profile in patients with HCC in comparison with people without HCC. The volunteers were: patients with HCC (n=81) and a healthy control group (n=90). The expression of OCT3was studied using the qRT-PCR method. The methylation profile was evaluated by genomic DNA using methylation specific PCR (MSP) method. The expression level of OCT3 marker mRNA in patients has decreased significantly compared to healthy individuals (0.58 ± 0.311 vs 1.20 ± 0.355, P <0.001). No significant statistical relationship was found between demographic data and OCT3 expression in participants (P >0.05). The amount of methylation (UM + MM) in cancer patients has raised vs controls (P <0.001) and has increased the risk of cancer (OR=0.379, 95% CI=1.171-2.839, P <0.001, and OR=2.727, 95% CI=1.251-5.945, P <0.001, respectively).Changes in OCT3 levels appear to be associated with HCC. Also, changing the methylation pattern of this gene can reveal HCC pathology.
Description
Keywords
Hepatocellular carcinoma, Methylation, Organic cation transporters 3, Quantitative real-time PCR
Citation
Heidari Zahra, Asemi-Rad Azam, Moudi Bita, Mahmoudzadeh-Sagheb Hamidreza. Association between the organic cation transporter 3 methylation and hepatocellular carcinoma. Indian Journal of Biochemistry & Biophysics. 2023 Mar; 60(3): 224-230