Characterization of the pili isolated from Vibrio parahaemolyticus O3:K6.

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dc.contributor.authorNakasone, Nen_US
dc.contributor.authorInsisengmay, Sen_US
dc.contributor.authorIwanaga, Men_US
dc.date.accessioned2009-05-27T16:26:31Z
dc.date.available2009-05-27T16:26:31Z
dc.date.issued2000-06-29en_US
dc.descriptionThe Southeast Asian Journal of Tropical Medicine and Public Health.en_US
dc.description.abstractPilus of Vibrio parahaemolyticus O3:K6 strain LVP9 belonging to the newly identified clone was purified and characterized. The molecular mass of the pilin was estimated to be about 18 kDa by SDS-PAGE, and the isoelectric point of the pilin was 5.0 +/- 0.2. The LVP9 pili were antigenically different from the other V. parahaemolyticus Na2 pili and Ha7 pili as previously reported, nevertheless all three had indistinguishable morphology and shared a high degree of homology in their N-terminal amino acid sequences. Strain LVP9 and its purified pili did not agglutinate human and rabbit erythrocytes. The LVP9 organisms and the purified pili were adhesive to the rabbit intestine. The adhesion was inhibited by pretreatment of the rabbit intestine with the purified pili or by pretreatment of the organisms with the Fab fractions of anti-pilus antibody. These results indicate that the LVP9 pilus is an adherent factor to the rabbit intestine.en_US
dc.description.affiliationDepartment of Bacteriology, Faculty of Medicine, University of the Ryukyus, Nishihara, Okinawa, Japan.en_US
dc.identifier.citationNakasone N, Insisengmay S, Iwanaga M. Characterization of the pili isolated from Vibrio parahaemolyticus O3:K6. The Southeast Asian Journal of Tropical Medicine and Public Health. 2000 Jun; 31(2): 360-5en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/35217
dc.language.isoengen_US
dc.source.urihttps://www.tm.mahidol.ac.th/seameo/2000_31_2/27-2465.pdfen_US
dc.subject.meshAmino Acid Sequenceen_US
dc.subject.meshBacterial Adhesionen_US
dc.subject.meshFimbriae Proteinsen_US
dc.subject.meshFimbriae, Bacterial --chemistryen_US
dc.subject.meshHemagglutinationen_US
dc.subject.meshIsoelectric Pointen_US
dc.subject.meshMembrane Proteins --chemistryen_US
dc.subject.meshMicroscopy, Immunoelectronen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshMolecular Weighten_US
dc.subject.meshSerotypingen_US
dc.subject.meshVibrio parahaemolyticus --classificationen_US
dc.titleCharacterization of the pili isolated from Vibrio parahaemolyticus O3:K6.en_US
dc.typeJournal Articleen_US
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