Intratypic differentiation & partial nucleotide sequencing of poliovirus isolates of northern India.

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dc.contributor.authorDhole, T Nen_US
dc.contributor.authorKapoor, Aen_US
dc.contributor.authorAgarwal, Jen_US
dc.contributor.authorIsomura, Sen_US
dc.contributor.authorKew, O Men_US
dc.date.accessioned2000-05-16en_US
dc.date.accessioned2009-05-27T07:11:06Z
dc.date.available2000-05-16en_US
dc.date.available2009-05-27T07:11:06Z
dc.date.issued2000-05-16en_US
dc.description.abstractThe potential resolving power of molecular epidemiological studies has enhanced the precision and reliability of poliovirus (PV) surveillance. PV has an error prone RNA polymerase responsible for rapid evolution of genome (approximately 10(-2) nt substitution/site/year), during inter and intra-human passages. The present study included a serotyped panel of 60 PV (42 PV type-1, 13 PV type-2 and 5 PV type-3) isolated during 1997. They were differentiated into vaccine (Sabin) and wild strains by two methods viz., genotype specific RNA probe hybridization (Rpro-Hy) based on genotypic variability; and ELISA that uses cross-absorbed antiserum (Pab-E) based on phenotypic variability. For obtaining information on molecular epidemiology, partial nucleotide sequencing (VP1/2A region) of five clinical PV isolates was also done. Three of the 60 isolates (two PV type-1 and one PV type-3) intratyped, could not be differentiated correctly by either method. Genotypic characterization of PV isolates was done for confirmation of intratyping results. All five wild PV1 sequenced belonged to the same genotype (> 85% homology) and sequence divergence among the strains was < or = 4.5 per cent. This indicated circulation of a single genetic lineage in the area.en_US
dc.description.affiliationDepartment of Microbiology, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Lucknow.en_US
dc.identifier.citationDhole TN, Kapoor A, Agarwal J, Isomura S, Kew OM. Intratypic differentiation & partial nucleotide sequencing of poliovirus isolates of northern India. Indian Journal of Medical Research. 2000 May; 111(): 151-6en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/19656
dc.language.isoengen_US
dc.source.urihttps://icmr.nic.in/ijmr/ijmr.htmen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshChilden_US
dc.subject.meshChild, Preschoolen_US
dc.subject.meshGenome, Viralen_US
dc.subject.meshHumansen_US
dc.subject.meshIndia --epidemiologyen_US
dc.subject.meshInfanten_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshPoliomyelitis --epidemiologyen_US
dc.subject.meshPoliovirus --geneticsen_US
dc.subject.meshRNA, Viral --geneticsen_US
dc.subject.meshSequence Analysisen_US
dc.titleIntratypic differentiation & partial nucleotide sequencing of poliovirus isolates of northern India.en_US
dc.typeJournal Articleen_US
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