Prevalence of group A genotype human rotavirus among children with diarrhoea in Nepal, 2009–2011.
Loading...
Date
2012-10
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Background: Rotavirus as a causative agent of childhood diarrhea is known to cause serious
illness among children less than 5 years of age. This study examined the epidemiology of rotavirus
disease burden and diversity of G and P genotypes of rotavirus in Nepal.
Methods: Stool samples were tested for rotavirus by Enzyme Immuno Assay and Group A rotaviruses
were detected by using both ELISA and RT-PCR in 2718 samples between 2009 and 2011.
Results: Rotavirus was more frequently detected among inpatients (28.5%) than outpatients
(15.2%). Over the three-year study period, 653 (24.4%) cases were positive for rotavirus by
ELISA. Genotyping by RT-PCR was done on 638 samples. The most prevalent genotype was G12P
[6] (60.4%). Mixed infections were not uncommon (14% in 2009, 29% in 2010 an 7% in 2011).
However, 41 were partially typed and 23 were completely untyped over the study period.
Conclusions: This study highlights the rotavirus disease burden and diversity of rotavirus strains
circulating in Nepal. Continued sentinel surveillance will provide useful information to policy makers
with regard to rotavirus vaccine introduction.
Description
Keywords
Human rotavirus, prevalence, genotype, RT-PCR
Citation
WHO South-East Asia Journal of Public Health 2012; 1(4): 432-440.