Cytochrome P450 1A activity in liver and fixed wavelength fluorescence detection of polycyclic aromatic hydrocarbons in the bile of tongue-fish (Cynoglossus acrolepidotus, Bleeker) in relation to petroleum hydrocarbons in the eastern Gulf of Thailand.

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dc.contributor.authorCheevaporn, Voraviten_US
dc.contributor.authorBeamish, F William Hen_US
dc.date.accessioned2007-10-15en_US
dc.date.accessioned2009-06-02T04:21:42Z
dc.date.available2007-10-15en_US
dc.date.available2009-06-02T04:21:42Z
dc.date.issued2007-10-15en_US
dc.description.abstractThis investigation was conducted in an area of oil spill along the east coast of Thailand to examine the relations among cytochrome P450 1A activity in liver and PAHs in the bile of the tonguefish and petroleum hydrocarbons in the sediments. PAH sediment concentrations in the reference and oil spill areas were 5.03 +/- 0.42 and 0.21 +/- 0.043 microg(-1) dry weight respectively Cytochrome activity in fish liver from oil spill area was 45.40 +/- 3.50 pmoles/ min/mg protein, almost threefold higher than that from the reference sites. Flourescense detection in bile metabolites at the oil spill area, 69.8 +/- 9.9 flourescense unit was significantly higher than that at the reference sites, 22.9 +/- 5.5 and 22.2 +/- 3.5 fluorescence unit. A strong correlation was found among cytochrome P450 1A activity in liver, PAH of bile metabolites and petroleum hydrocarbons. Both cytochrome and bile metabolites activity decreased seaward varying to the distance from the oil polluted area. We concluded that both detections in tonguefish can be regarded as a complementary biomarkers for the exposure of PAHs in tropical marine environments.en_US
dc.description.affiliationDepartment of Aquatic Science, Faculty of Science, Burapha University, Chonburi-20131, Thailand. voravit@buu.ac.then_US
dc.identifier.citationCheevaporn V, Beamish FW. Cytochrome P450 1A activity in liver and fixed wavelength fluorescence detection of polycyclic aromatic hydrocarbons in the bile of tongue-fish (Cynoglossus acrolepidotus, Bleeker) in relation to petroleum hydrocarbons in the eastern Gulf of Thailand. Journal of Environmental Biology. 2007 Oct; 28(4): 701-5en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/113716
dc.language.isoengen_US
dc.source.urihttps://www.geocities.com/j_environ_biol/en_US
dc.subject.meshAnimalsen_US
dc.subject.meshBile --chemistryen_US
dc.subject.meshCytochrome P-450 Enzyme System --metabolismen_US
dc.subject.meshFishesen_US
dc.subject.meshGeologic Sediments --chemistryen_US
dc.subject.meshLiver --enzymologyen_US
dc.subject.meshPetroleum --analysisen_US
dc.subject.meshPolycyclic Compounds --analysisen_US
dc.subject.meshSpectrometry, Fluorescenceen_US
dc.subject.meshWater Pollutants, Chemical --analysisen_US
dc.titleCytochrome P450 1A activity in liver and fixed wavelength fluorescence detection of polycyclic aromatic hydrocarbons in the bile of tongue-fish (Cynoglossus acrolepidotus, Bleeker) in relation to petroleum hydrocarbons in the eastern Gulf of Thailand.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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