Clonal similarities and sequence-type diversity of invasive and carriage Streptococcus pneumoniae in India among children under 5 Years

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dc.contributor.authorVarghese, Ren_US
dc.contributor.authorNeeravi, Aen_US
dc.contributor.authorSubramanian, Nen_US
dc.contributor.authorPavithra, Ben_US
dc.contributor.authorKavipriya, Aen_US
dc.contributor.authorKumar, JLen_US
dc.contributor.authorGirish, Kumar, C, Pen_US
dc.contributor.authorJeyraman, Yen_US
dc.contributor.authorKarthik, Gen_US
dc.contributor.authorVerghese, VPen_US
dc.contributor.authorVeeraraghavan, Ben_US
dc.date.accessioned2020-04-23T07:38:38Z
dc.date.available2020-04-23T07:38:38Z
dc.date.issued2019-09
dc.description.abstractBackground: Pneumococcal pneumonia is one of the major causes of mortality in children less than 5 years in Asia, especially in India. Available PCVs have less serotype coverage in India compared to western countries. Moreover, the baseline pneumococcal serotype and sequence type data is limited and available data doesn't represent the entire India. With this background we aimed to characterize invasive and carriage isolates of S. pneumoniae from a tertiary care hospital in South India. Materials and Methods: A total of 221 S. pneumoniae isolates, invasive (n=138) and carriage (n=83) between the time period of 2012-2018 were included. Isolates was identified and confirmed using standard laboratory protocols. Serotyping was performed by Customized sequential multiplex PCR and MLST as described in www.pubmlst.org. Results: The major serotypes were 19F, 6B, 14, 6A and 19A and the sequence types (ST) were ST63, 236 and 230. Predominant STs in invasive was ST 63 whereas in carriage were ST4894 and 1701. High level ST diversity in carriage was observed. Majority of the STs were SLVs or DLVs of previously reported STs or PMEN clones. Phylogenetic analyses of the STs revealed gradual expansion of three PMEN CCs CC320, 63 and 230. Conclusion: The vaccine serotypes were the predominant ones found to be associated with IPD, PMEN clones, new STs and antimicrobial resistance. Accordingly, PCV13 is expected to provide invasive serotype coverage of 75% in Indian children less than 5 years. This study provides baseline serotype and sequence type data prior to the introduction of PCV in South India.en_US
dc.identifier.affiliationsDepartment of Clinical Microbiology, Christian Medical College, Vellore, Tamil Nadu, Indiaen_US
dc.identifier.affiliationsDepartment of Paediatrics, Christian Medical College, Chennai, Tamil Nadu, Indiaen_US
dc.identifier.affiliationsICMR, National Institute of Epidemiology, Chennai, Tamil Nadu, Indiaen_US
dc.identifier.affiliationsDepartment of Medicine, Christian Medical College, Vellore, Tamil Nadu, Indiaen_US
dc.identifier.citationVarghese R, Neeravi A, Subramanian N, Pavithra B, Kavipriya A, Kumar JL, Girish Kumar C P, Jeyraman Y, Karthik G, Verghese VP, Veeraraghavan B. Clonal similarities and sequence-type diversity of invasive and carriage Streptococcus pneumoniae in India among children under 5 Years. Indian Journal of Medical Microbiology. 2019 Sep; 37(3): 358-362en_US
dc.identifier.issn0255-0857
dc.identifier.issn1998-3646
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/198913
dc.languageenen_US
dc.publisherIndian Association of Medical Microbiologistsen_US
dc.relation.issuenumber3en_US
dc.relation.volume37en_US
dc.source.urihttps://dx.doi.org/10.4103/ijmm.IJMM_19_348en_US
dc.subjectIndiaen_US
dc.subjectmultilocus sequence typingen_US
dc.subjectpneumococcal conjugate vaccines 13en_US
dc.subjectpneumococcal sequence typesen_US
dc.subjectStreptococcus pneumoniaeen_US
dc.titleClonal similarities and sequence-type diversity of invasive and carriage Streptococcus pneumoniae in India among children under 5 Yearsen_US
dc.typeJournal Articleen_US
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