Recognition of major methicillin-resistant Staphylococcus aureus (MRSA) clones by molecular surveillance in Srinagarind Hospital, Khon Kaen

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dc.contributor.authorBoualay Norchaleun; Graduate School, Khon Kaen Universityen_US
dc.contributor.authorChotchana Wilailuckana; Centre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen Universityen_US
dc.contributor.authorNuttanan Hongsrichan; Centre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen Universityen_US
dc.contributor.authorWanlop Kaewkes; Center for Emerging Diseases, Faculty of Medicine, Khon Kaen Universityen_US
dc.contributor.authorChokchai Wilachai; Clinical Microbilogy Laboratory, Srinagarind Hospital, Khon Kaen Universityen_US
dc.contributor.authorAroonlug Lulitanond; Centre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen Universityen_US
dc.contributor.authorAroonwadee Chanawong; Centre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen Universityen_US
dc.contributor.authorPrakai Pitak; Infection control unit, Srinagarind Hospital, Khon Kaen Universityen_US
dc.contributor.authorJanpen Bourpoern; Infection control unit, Srinagarind Hospital, Khon Kaen Universityen_US
dc.contributor.authorSaisamorn Poldongnauk; Infection control unit, Srinagarind Hospital, Khon Kaen Universityen_US
dc.date.accessioned2011-02-20T04:32:57Z
dc.date.available2011-02-20T04:32:57Z
dc.date.created2010-04-11en_US
dc.date.issued2010-04-11en_US
dc.description.abstractMethicillin-resistant Staphylococcus aureus, a well-known nosocomial pathogen in tertiary healthcare facilities, can cause severe life-threatening symptoms. Nowadays, prevention and control of outbreaks related to hospital-acquired infections need molecular information to distinguish and definitely define a real etiology. For the last decade, molecular techniques have been developed and applied to an epidemiological study of infectious diseases. Among them, polymerase chain reaction-based typing techniques are most feasible to be used as molecular tools in clinical microbiology laboratory in Thailand. In this study, PCR-based typing methods, including SCCmec typing, variable numbers of tandem repeats typing of hypervariable region downstream of mecA (HVR) locus and spa gene, were applied in order to determine genetic background, and major endemic clones in Srinagarind Hospital, Khon Kaen. A total of 247 MRSA isolated from 124 patients of Srinagarind Hospital during July 2007 through December 2008 were characterized by the PCR-based typing methods described above. Five SCCmec types were identified as type-III (60.7%), type-IIIA (30.8%), type-II SCCmec (6%), type-III DCS (1.7%), and type-I variant with class C mec complex (0.9%), respectively. HVR and spa typing differentiated MRSA into 5 and 10 groups, respectively. Combination of all genetic markers could identify two major clones, III-15-7 (43.6%), and IIIA-7-7 (22.2%). Medical wards and medical intensive care unit were considered as endemic areas of these two clones. Information in this study may be applied to infection control measure and lead to development of suitable PCR-typing techniques for MRSA in clinical laboratory. en_US
dc.identifier.citationJournal of Medical Technology and Physical Therapy; Volume 22 Number 1, January - April 2010; 26-36en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/130895
dc.language.isoen_USen_US
dc.publisherKhon Kaen Universityen_US
dc.rightsKhon Kaen Universityen_US
dc.source.urihttps://thailand.digitaljournals.org/index.php/JMTPT/issue/archiveen_US
dc.source.urihttps://thailand.digitaljournals.org/index.php/JMTPT/article/view/3377en_US
dc.titleRecognition of major methicillin-resistant Staphylococcus aureus (MRSA) clones by molecular surveillance in Srinagarind Hospital, Khon Kaenen_US
dc.typeORIGINAL ARTICLEen_US
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