Occurrence and molecular characterization of enteropathogenic Escherichia coli serotypes isolated from children with diarrhoea in Najaf, Iraq.

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dc.contributor.authorAl-Hilali, Samer A
dc.contributor.authorAlmohana, Ali M
dc.date.accessioned2012-12-14T09:01:22Z
dc.date.available2012-12-14T09:01:22Z
dc.date.issued2011-10
dc.description.abstractPurpose: Enteropathogenic Escherichia coli (EPEC) are among the most important pathogens infecting children worldwide and are one of the main causes of diarrhoea. The study was carried out to investigate the occurrence of EPEC as a cause of infectious diarrhoea in children younger than 2 years of age and characterize their virulence genes. Materials and Methods: During the study period, a total of 656 faecal specimens from children with diarrhoea and 54 from healthy children were analyzed. E. coli isolates were serotypically identified with EPEC polyvalent and monovalent antisera. The isolated EPEC were examined for the presence of the attaching and effacing (eaeA), bundle-forming pilus (bfpA), Shiga like toxins (stx1 and stx2 ), enterohaemorrhagic E. coli enterohaemolysin (EHEC hlyA) and EPEC adherence factor (EAF) genes by the PCR assay. Results: The study has shown that 22 (3.4%) had diarrhoea due to EPEC, while no EPEC isolates were detected in asymptomatic children. The highest number of the EPEC isolated belonging to polyvalent 2. The primers encoding virulence genes were subjected to all the EPEC isolates. Only 9.1%, 27.3%, and 9.1% isolates gave positive re sults with intimin (eaeA), bfbA and (EAF) genes, respectively. None of the isolates were positive for stx 1, stx 2, and hlyA genes. Typical EPEC (eaeA +, bfpA +) was diagnosed in two isolates, while, atypical EPEC was manifested in four isolates. Conclusions: According to the results, the frequency of EPEC isolates in Najaf was lower than what has been suspected and the investigation including the use of molecular technique and serotyping, are necessary to allow precise identification and epidemiological study of these pathogens.en_US
dc.identifier.citationAl-Hilali Samer A, Almohana Ali M. Occurrence and molecular characterization of enteropathogenic Escherichia coli serotypes isolated from children with diarrhoea in Najaf, Iraq. Indian Journal of Medical Microbiology. 2011 Oct-Dec; 29(4): 383-388.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/143861
dc.language.isoenen_US
dc.source.urihttps://www.ijmm.org/article.asp?issn=0255-0857;year=2011;volume=29;issue=4;spage=383;epage=388;aulast=Alen_US
dc.subjectAtypical EPECen_US
dc.subjectbundle-forming pilusen_US
dc.subjectenteropathogenic Escherichia colien_US
dc.subjectintiminen_US
dc.subjecttypical Enteropathogenic Escherichia colien_US
dc.subject.meshEnteropathogenic Escherichia coli --classification
dc.subject.meshEnteropathogenic Escherichia coli --immunology
dc.subject.meshEnteropathogenic Escherichia coli --isolation & purification
dc.subject.meshEnteropathogenic Escherichia coli --pathogenicity
dc.subject.meshEscherichia coli Infections --microbiology
dc.subject.meshEscherichia coli Proteins --genetics
dc.subject.meshFeces --microbiology
dc.subject.meshGenotype
dc.subject.meshHumans
dc.subject.meshInfant
dc.subject.meshIraq
dc.subject.meshPolymerase Chain Reaction
dc.subject.meshSerotyping
dc.subject.meshVirulence Factors --genetics
dc.titleOccurrence and molecular characterization of enteropathogenic Escherichia coli serotypes isolated from children with diarrhoea in Najaf, Iraq.en_US
dc.typeArticleen_US
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