o-Phthalaldehyde as a probe in the active site of phosphoenolpyruvate carboxylase.

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dc.contributor.authorMaralihalli, G Ben_US
dc.contributor.authorBhagwat, A Sen_US
dc.date.accessioned1990-06-01en_US
dc.date.accessioned2009-05-27T09:37:32Z
dc.date.available1990-06-01en_US
dc.date.available2009-05-27T09:37:32Z
dc.date.issued1990-06-01en_US
dc.description.abstractModification of phosphoenolpyruvate carboxylase with o-phthalaldehyde (OPA) resulted in rapid and irreversible inactivation exhibiting biphasic reaction kinetics. The kinetic analysis and correlation of spectral changes with activity indicated that inactivation by OPA results from the modification of two lysine and two cysteine residues per subunit of the enzyme. PEP plus Mg2+ offered substantial protection against modification. Some of the effectors also gave appreciable protection against modification indicating that the residues may be located at or close to the active site. Thus, the results indicate formation of two isoindoles showing the proximity of the essential lysine and cysteine residues at the active site.en_US
dc.description.affiliationMolecular Biology & Agriculture Division, Bhabha Atomic Research Centre, Trombay, Bombay.en_US
dc.identifier.citationMaralihalli GB, Bhagwat AS. o-Phthalaldehyde as a probe in the active site of phosphoenolpyruvate carboxylase. Indian Journal of Biochemistry & Biophysics. 1990 Jun; 27(3): 141-5en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/28071
dc.language.isoengen_US
dc.source.urihttps://https://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijbb/ijbb0.aspen_US
dc.subject.meshAldehydesen_US
dc.subject.meshBinding Sites --physiologyen_US
dc.subject.meshCarboxy-Lyases --metabolismen_US
dc.subject.meshKineticsen_US
dc.subject.meshPhosphoenolpyruvate Carboxylase --antagonists & inhibitorsen_US
dc.subject.meshZea mays --enzymologyen_US
dc.subject.mesho-Phthalaldehydeen_US
dc.titleo-Phthalaldehyde as a probe in the active site of phosphoenolpyruvate carboxylase.en_US
dc.typeJournal Articleen_US
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