Morphological and molecular identification of the freshwater crab, Barytelphusa cunicularis from Kanniyakumari, India

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dc.contributor.authorRaj, T.G. Teeni Janeten_US
dc.contributor.authorSuganthi, A. Shylaen_US
dc.contributor.authorRaj, T.G. Tyni Joiceen_US
dc.contributor.authorAnilkumar, G.en_US
dc.date.accessioned2024-11-30T11:59:23Z
dc.date.available2024-11-30T11:59:23Z
dc.date.issued2023-11
dc.description.abstractAim: The present study aims to assess the taxonomical identification of commercially important freshwater crab, Barytelphusa cunicularis from Kumba River, Kanniyakumari using morphological and molecular tools. Methodology: Samples of B. cunicularis were collected from Kumba River flowing in Kilamalai, Kanniyakumari district. The species identification was carried out through morphological keys constructed out of a coding matrix and phylogenetic tree (Maximum Parsimony) using Mesquite and PAUP4 software. 18S rRNA sequence was subjected to BLAST analysis, and the phylogenetic tree was constructed through Maximum Likelihood method using MEGA 11 software. Pairwise genetic distance of the species (p-distance,p = nd/n) was also assessed by comparing the K2P values involving phylogenetically close and distant relatives. Results: Coding matrix prepared using the morphological keys, raised out of 28 distinctive characteristics of B. cunicularis and comparison with its phylogenetic relatives, have brought in valuable information on status of the species. These findings were further established by Maximum Likelihood Analysis, using the PCR amplicons of 18S rRNA. The phylogram prepared out of the sequence clearly reveals the candidate species' phylogenetic proximity to other members of the genus Barytelphusa. Further, the species' monophyletic status (with a BS value of 81%) suggests its early divergence from its congeners. The K2P pairwise genetic 'p' distance analysis (p = nd/n) of the 18S rRNA has helped us not only to further ascertain the extent of its genetic identity with its congeners, but as well has clearly provided us with the valuable cues per precise identification of the species. Interpretation: Along with the morphological parameters, the present study, using molecular tools, provides valuable information for precise identification of the commercially important freshwater crab.en_US
dc.identifier.affiliationsDepartment of Zoology, Holy Cross College (Autonomous), Nagercoil, Affiliated to Manonmaniam Sundaranar University, Tirunelveli -627 012, Indiaen_US
dc.identifier.affiliationsDepartment of Zoology, Holy Cross College (Autonomous), Nagercoil-629 004, Indiaen_US
dc.identifier.affiliationsDepartment of Zoology, Holy Cross College (Autonomous), Nagercoil, Affiliated to Manonmaniam Sundaranar University, Tirunelveli -627 012, Indiaen_US
dc.identifier.affiliationsSchool of Biosciences and Technology, Vellore Institute of Technology, Vellore-632 014, Indiaen_US
dc.identifier.citationRaj T.G. Teeni Janet, Suganthi A. Shyla, Raj T.G. Tyni Joice, Anilkumar G.. Morphological and molecular identification of the freshwater crab, Barytelphusa cunicularis from Kanniyakumari, India. Journal of Environmental Biology. 2023 Nov; 44(6): 804-810en_US
dc.identifier.issn0254-8704
dc.identifier.issn2394-0379
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/238479
dc.languageenen_US
dc.publisherTriveni Enterprisesen_US
dc.relation.issuenumber6en_US
dc.relation.volume44en_US
dc.source.urihttps://doi.org/10.22438/jeb/44/6/5088en_US
dc.subjectBarytelphusa cunicularisen_US
dc.subjectCrustaceaen_US
dc.subjectFreshwater craben_US
dc.subjectGecarcinucidaeen_US
dc.subjectPhylogramen_US
dc.subjectTaxonomyen_US
dc.titleMorphological and molecular identification of the freshwater crab, Barytelphusa cunicularis from Kanniyakumari, Indiaen_US
dc.typeJournal Articleen_US
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