Inhibition of fatty acid synthase supresses osteosarcoma cell invasion and migration.

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dc.contributor.authorLiu, Zhi Li
dc.contributor.authorZhou, Yang
dc.contributor.authorLuo, Qing Feng
dc.contributor.authorHu, MinWei
dc.contributor.authorWang, Gao
dc.contributor.authorHuang, Shan Hu
dc.contributor.authorShu, Yong
dc.date.accessioned2012-10-17T04:21:07Z
dc.date.available2012-10-17T04:21:07Z
dc.date.issued2012-04
dc.description.abstractBackground : Fatty acid synthase (FASN) is overexpressed in a variety of human cancers, and may be involved in cancer metastasis. Hence, the strategies targeted on FASN may have therapeutic potential for treating cancer metastasis. Objectives : The aim of this study is to investigate the correlation of FASN expression with metastasis in human osteosarcoma. Materials and Methods : Human osteosarcoma cell lines U2-OS and osteosarcoma biopsy specimens were employed in this study. The expression of FASN protein in osteosarcoma specimens was detected by IHC (immunohistochemistry) and the relationship with metastasis was analyzed. We performed the cerulenin, an inhibitor of FASN, to inhibit FASN expression in U2-OS cells. Western blot and RT-PCR were performed to investigate the expression of FASN in U2-OS cells. Cells mobility was detected by wound healing and Transwell assays. Results : Results showed that the FASN expression level in the cases with pulmonary metastases was significantly higher than in those without metastasis. In vitro, the invasion and migration of U2-OS cells were suppressed by inhibiting FASN. Our findings suggested that FASN may be involved in osteosarcoma metastasisen_US
dc.identifier.citationLiu Zhi Li, Zhou Yang, Luo Qing Feng, Hu MinWei, Wang Gao, Huang Shan Hu, Shu Yong. Inhibition of fatty acid synthase supresses osteosarcoma cell invasion and migration. Indian Journal of Pathology & Microbiology. 2012 Apr-Jun 55(2): 163-169.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/142214
dc.language.isoenen_US
dc.source.urihttps://www.ijpmonline.org/article.asp?issn=0377-4929;year=2012;volume=55;issue=2;spage=163;epage=169;aulast=Liuen_US
dc.subjectFatty acid synthaseen_US
dc.subjectimmunohistochemistryen_US
dc.subjectmetastasisen_US
dc.subjectosteosarcomaen_US
dc.subject.meshAdolescent
dc.subject.meshAntineoplastic Agents --pharmacology
dc.subject.meshBlotting, Western
dc.subject.meshCell Movement --drug effects
dc.subject.meshCell Proliferation --drug effects
dc.subject.meshCells, Cultured
dc.subject.meshCerulenin --pharmacology
dc.subject.meshChild
dc.subject.meshChild, Preschool
dc.subject.meshEnzyme Inhibitors --pharmacology
dc.subject.meshFatty Acid Synthetase Complex, Type I --antagonists & inhibitors
dc.subject.meshFemale
dc.subject.meshGene Expression Profiling
dc.subject.meshHumans
dc.subject.meshImmunohistochemistry
dc.subject.meshMale
dc.subject.meshOsteosarcoma --pathology
dc.subject.meshOsteosarcoma --secondary
dc.subject.meshReal-Time Polymerase Chain Reaction
dc.titleInhibition of fatty acid synthase supresses osteosarcoma cell invasion and migration.en_US
dc.typeArticleen_US
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