Real-time PCR analysis of dog cerebrospinal fluid and saliva samples for ante-mortem diagnosis of rabies.

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dc.contributor.authorSaengseesom, Wachirapornen_US
dc.contributor.authorMitmoonpitak, Channarongen_US
dc.contributor.authorKasempimolporn, Songsrien_US
dc.contributor.authorSitprija, Visithen_US
dc.date.accessioned2009-05-27T15:31:15Z
dc.date.available2009-05-27T15:31:15Z
dc.date.issued2007-01-02en_US
dc.descriptionThe Southeast Asian Journal of Tropical Medicine and Public Health.en_US
dc.description.abstractThe use of a 10-day observation to determine whether a dog is rabid is standard practice. This study was conducted in order to look for evidence of rabies vius in saliva and cerebrospinal fluid (CSF) of suspected live rabid dogs at the time of quarantine by using a SYBR Green real-time RT-PCR based assay for the detection of rabies virus RNA. Saliva and CSF of dogs were collected once on the day of admission for the 10-day quarantine. All test dogs were or became ill and died of rabies within the observation period. Thirteen of 15 dogs (87%) had saliva samples that were positive for rabies RNA. Two dogs with furious rabies had negative saliva samples. Positive CSF samples were found in 4 of 15 dogs (27%) whose saliva samples were positive. The time from sample collection to result was less than 5 hours. Because virus may be absent or present at very low level in both clinical fluids, samples taken for ante-mortem diagnosis cannot definitively rule out rabies.en_US
dc.description.affiliationQueen Saovabha Memorial Institute (WHO Collaborating Center for Research on Rabies), Thai Red Cross Society, Bangkok, Thailand.en_US
dc.identifier.citationSaengseesom W, Mitmoonpitak C, Kasempimolporn S, Sitprija V. Real-time PCR analysis of dog cerebrospinal fluid and saliva samples for ante-mortem diagnosis of rabies. The Southeast Asian Journal of Tropical Medicine and Public Health. 2007 Jan; 38(1): 53-7en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/32987
dc.language.isoengen_US
dc.source.urihttps://www.tm.mahidol.ac.th/seameo/2007_38_1/09-3914.pdfen_US
dc.subject.meshAnimalsen_US
dc.subject.meshComputer Systemsen_US
dc.subject.meshDiagnosisen_US
dc.subject.meshDogsen_US
dc.subject.meshObservationen_US
dc.subject.meshPredictive Value of Testsen_US
dc.subject.meshQuarantineen_US
dc.subject.meshRNA, Viral --analysisen_US
dc.subject.meshRabies --cerebrospinal fluiden_US
dc.subject.meshRabies virus --geneticsen_US
dc.subject.meshReverse Transcriptase Polymerase Chain Reaction --methodsen_US
dc.subject.meshSaliva --virologyen_US
dc.subject.meshThailanden_US
dc.subject.meshTime Factorsen_US
dc.titleReal-time PCR analysis of dog cerebrospinal fluid and saliva samples for ante-mortem diagnosis of rabies.en_US
dc.typeJournal Articleen_US
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