A novel multiplex PCR for molecular characterization of methicillin resistant Staphylococcus aureus recovered from Jeddah, Kingdom of Saudi Arabia.

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dc.contributor.authorMoussa, I M I
dc.contributor.authorKabli, S A
dc.contributor.authorHemeg, H A
dc.contributor.authorAl-Garni, S M
dc.contributor.authorShibl, A M
dc.date.accessioned2012-12-19T05:33:38Z
dc.date.available2012-12-19T05:33:38Z
dc.date.issued2012-07
dc.description.abstractBackground: Molecular characterization of staphylococcal cassette chromosome mec (SCCmec) types of methicillin-resistant Staphylococcus aureus (MRSA) is very essential for studying the epidemiology of MRSA. Objectives: This study reports two multiplex PCR for molecular typing of MRSA collected from Jeddah, Kingdom of Saudi Arabia. Materials and Methods: A total of 101 clinical isolates of strains were collected from major hospital laboratories and public health centres, Jeddah, Kingdom of Saudi Arabia during the period from August 2009 to May 2011. All the strains were tested phenotypically by conventional methods and genotypically by a novel multiplex PCR targeting at the same time S. aureus 16S rRNA, Panton - valentine leucocidin (PVL) and mecA resistance genes. All the strains were tested also by multiplex PCR for typing of SCC mec types. Results: All the 101 strains previously identified phenotypically as S. aureus with bacteriological examination were positive for amplification of 756 base pair fragments specific for 16S rRNA of S. aureus. Moreover, all the strains were positive for amplification of 1339 base pair fragments specific for mecA gene, while only 38 strains (37.6%) showed positive amplification of 433 base pair fragments specific for PVL gene. The most predominant SCC mec type among the examined isolates is type V 43 (42.5) followed by SCCmec type III 39 (38.6%). Conclusion: The newly modified multiplex PCR is rapid and sensitive method for detection of MRSA. Moreover, the most predominant SCC mec type among the examined isolates from Jeddah, King Saudi Arabia is type V (42.5%), followed by Type III (38.6%).en_US
dc.identifier.citationMoussa I M I, Kabli S A, Hemeg H A, Al-Garni S M, Shibl A M. A novel multiplex PCR for molecular characterization of methicillin resistant Staphylococcus aureus recovered from Jeddah, Kingdom of Saudi Arabia. Indian Journal of Medical Microbiology. 2012 Jul-Sept; 30(3): 296-301.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/143973
dc.language.isoenen_US
dc.source.urihttps://www.ijmm.org/article.asp?issn=0255-0857;year=2012;volume=30;issue=3;spage=296;epage=301;aulast=Moussaen_US
dc.subjectMRSAen_US
dc.subjectmultiplex-PCRen_US
dc.subjectPVL geneen_US
dc.subjectStaphylococcus aureusen_US
dc.subjectstaphylococcal cassette chromosome mecen_US
dc.subject.meshBacterial Proteins --genetics
dc.subject.meshBacterial Toxins --genetics
dc.subject.meshExotoxins --genetics
dc.subject.meshGenotype
dc.subject.meshHumans
dc.subject.meshLeukocidins --genetics
dc.subject.meshMethicillin-Resistant Staphylococcus aureus --classification
dc.subject.meshMethicillin-Resistant Staphylococcus aureus --genetics
dc.subject.meshMethicillin-Resistant Staphylococcus aureus --isolation & purification
dc.subject.meshMicrobial Sensitivity Tests
dc.subject.meshMolecular Typing --methods
dc.subject.meshMultiplex Polymerase Chain Reaction --methods
dc.subject.meshPhenotype
dc.subject.meshSaudi Arabia
dc.subject.meshSensitivity and Specificity
dc.subject.meshStaphylococcal Infections --microbiology
dc.subject.meshTime Factors
dc.titleA novel multiplex PCR for molecular characterization of methicillin resistant Staphylococcus aureus recovered from Jeddah, Kingdom of Saudi Arabia.en_US
dc.typeArticleen_US
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