Faecal bifidobacteria in Indian neonates & the effect of asymptomatic rotavirus infection during the first month of life.

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dc.contributor.authorBalamurugan, Ramadass
dc.contributor.authorMagne, Fabien
dc.contributor.authorBalakrishnan, Divya
dc.contributor.authorSuau, Antonia
dc.contributor.authorRamani, Sasirekha
dc.contributor.authorKang, Gagandeep
dc.contributor.authorRamakrishna, Balakrishnan S
dc.date.accessioned2011-12-05T04:01:09Z
dc.date.available2011-12-05T04:01:09Z
dc.date.issued2010-12
dc.description.abstractBackground & Objectives: Bifidobacteria colonize the gut after the first week of life and remain an important component of the gut microbiota in infancy. This study was carried out to characterize the diversity and number of bifidobacteria colonizing the gut in Indian neonates and to investigate whether asymptomatic infection with rotavirus in the first month of life affected gut colonization by bidifobacteria. Methods: DNA was isolated from faeces of 14 term-born neonates who were under surveillance for rotavirus infection. Bacterial and bifidobacterial diversity was evaluated by temporal temperature gradient electrophoresis (TTGE) of 16S rDNA amplified using total bacteria and bifidobacteria-specific primers. Real time PCR, targeting 16S rDNA, was used to quantitate faecal bifidobacteria and enterobacteria. Results: TTGE of conserved bacterial 16S rDNA showed 3 dominant bands of which Escherichia coli (family Enterobacteriaceae) and Bifidobacterium (family Bifidobacteriaceae) were constant. TTGE of Bifidobacterium genus-specific DNA showed a single band in all neonates identified by sequencing as Bifidobacterium longum subsp. infantis. Faecal bifidobacterial counts (log10 cfu/g faeces) ranged from 6.1 to 9.3 and enterobacterial counts from 6.3 to 9.5. Neonates without and with rotavirus infection in the first week of life did not show significant differences in the median count of bifidobacteria (log10 count 7.48 vs. 7.41) or enterobacteria (log10 count 8.79 vs. 7.92). Interpretation & Conclusions: B. longum subsp. infantis was the sole bifidobacterial species colonizing the gut of Indian neonates. Asymptomatic rotavirus infection in the first month of life was not associated with alteration in faecal bifidobacteria or enterobacteria.en_US
dc.identifier.citationBalamurugan Ramadass, Magne Fabien, Balakrishnan Divya, Suau Antonia, Ramani Sasirekha, Kang Gagandeep, Ramakrishna Balakrishnan S. Faecal bifidobacteria in Indian neonates & the effect of asymptomatic rotavirus infection during the first month of life. Indian Journal of Medical Research. 2010 Dec; 132(6): 721-727.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/135652
dc.language.isoenen_US
dc.source.urihttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3102461/en_US
dc.subjectBifidobacteriumen_US
dc.subjectcolonizationen_US
dc.subjectguten_US
dc.subjectneonatesen_US
dc.subjectrotavirusen_US
dc.subject.meshBifidobacterium --genetics
dc.subject.meshBiodiversity
dc.subject.meshDNA Primers --genetics
dc.subject.meshDNA, Ribosomal --genetics
dc.subject.meshDenaturing Gradient Gel Electrophoresis
dc.subject.meshFeces --microbiology
dc.subject.meshGastrointestinal Tract --microbiology
dc.subject.meshHumans
dc.subject.meshIndia
dc.subject.meshInfant, Newborn
dc.subject.meshRotavirus Infections --microbiology
dc.subject.meshSequence Analysis, DNA
dc.subject.meshSpecies Specificity
dc.subject.meshStatistics, Nonparametric
dc.titleFaecal bifidobacteria in Indian neonates & the effect of asymptomatic rotavirus infection during the first month of life.en_US
dc.typeArticleen_US
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