Multiplex PCR on leptospiral isolates from Kolenchery, Kerala, India.

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dc.contributor.authorSugathan, Sen_US
dc.contributor.authorVarghese, T Pen_US
dc.date.accessioned2005-04-02en_US
dc.date.accessioned2009-05-28T09:17:04Z
dc.date.available2005-04-02en_US
dc.date.available2009-05-28T09:17:04Z
dc.date.issued2005-04-02en_US
dc.description.abstractHuman leptospirosis causes severe multiorgan dysfunctions that may end in multiorgan failure and death. The methods in hand for diagnosis of leptospirosis like culture, ELISA and MAT only help to confirm the disease, and are of little value in early detection. The aim of this study was to find out if the two sets of primers described earlier could detect all the isolates from the area, for the purpose of using the resultant database for early detection of leptospires in future from clinical specimens. The study was done on culture isolates from Jan 2000 to June 2002 attending the department of medicine, MOSC medical college hospital, Kolenchery, Kerala, India. DNA of 45 culture isolates were amplified by multiplex PCR using two sets of previously described primers, G1, G2 and B 64-I, B 64-II. Specific amplifications of either 285 or 563 bp size were obtained from all isolates included in the study indicating the utility of the multiplex PCR in the rapid detection of leptospires in clinical samples.en_US
dc.description.affiliationDepartment of Microbiology, MOSC Medical College, Kolenchery, Kerala, India. sheelasugathan@yahoo.co.inen_US
dc.identifier.citationSugathan S, Varghese TP. Multiplex PCR on leptospiral isolates from Kolenchery, Kerala, India. Indian Journal of Medical Microbiology. 2005 Apr; 23(2): 114-6en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/53700
dc.language.isoengen_US
dc.source.urihttps://www.ijmm.orgen_US
dc.subject.meshDNA Primersen_US
dc.subject.meshHospitals, Countyen_US
dc.subject.meshHumansen_US
dc.subject.meshIndiaen_US
dc.subject.meshLeptospira --geneticsen_US
dc.subject.meshLeptospirosis --diagnosisen_US
dc.subject.meshPolymerase Chain Reaction --methodsen_US
dc.subject.meshSpecies Specificityen_US
dc.titleMultiplex PCR on leptospiral isolates from Kolenchery, Kerala, India.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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