Anti-biofilm and anti-adherence activity of Glm-U inhibitors.

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dc.contributor.authorSuman, Ethel
dc.contributor.authorD'souza, Sushma Janet
dc.contributor.authorJacob, Pyari
dc.contributor.authorSushruth, M R
dc.contributor.authorKotian, M Shashidhar
dc.date.accessioned2013-03-19T08:12:27Z
dc.date.available2013-03-19T08:12:27Z
dc.date.issued2011-09
dc.description.abstractBackground: Intravascular catheters and urinary catheters are an important source of hospital-acquired infections. Many microorganisms colonize indwelling catheters, including central venous catheters (CVCs) forming biofilms and cause infections that are difficult to treat. Although various methods have been employed to reduce biofilms, enzymes involved in bacterial cell wall synthesis could provide novel targets for the development of anti-biofilm agents. N-Acetylglucosamine-1-phosphate uridyltransferase (GlmU) is an essential enzyme in aminosugars metabolism and catalyzes the formation of uridine-diphospho-N-acetylglucosamine (UDP-GlcNAc), an important precursor in the peptidoglycan and lipopolysaccharide biosynthesis of Gram-positive and Gram-negative bacteria. Previous study has been conducted on the anti-biofilm effect of GlmU inhibitors such as N-ethyl maleimide (NEM) and NEM analogs along with a cationic polypeptide protamine sulfate (PS), which enhanced its anti-biofilm activity. AIM: The present study aimed at finding the effect of sub-inhibitory concentrations of N-ethyl maleimide (NEM) and protamine sulfate (PS) on the biofilms produced by Pseudomonas aeruginosa and Enterococcus spp. isolated from cases of catheter-associated UTI as well as Klebsiella pneumoniae and Staphylococcus aureus isolated from cases of catheter-related bloodstream infections (CRBSI). Materials and Methods: In order to enhance the activity of NEM and to develop a broad-spectrum anti-microbial composition, NEM (50 μg/ml) was combined with protamine sulfate (50 μg/ml) and tested for anti-biofilm activity using a standard quantitative biofilm assay method. Results and Conclusion: It was observed that NEM had no effect on the biofilm produced by Pseudomonas aeruginosa as well as by Enterococcus spp. NEM also caused a significant decrease in biofilm production by Staphylococcus aureus while it had no effect on the biofilm produced by Klebsiella pneumoniae. There was a significant synergistic inhibitory effect on Staphylococcus aureus and Enterococcus spp., whereas Pseudomonas aeruginosa and Klebsiella pneumoniae remained unaffected. Combination of GlmU inhibitor-plus-protamine sulfate failed to significantly reduce bacterial adherence of Pseudomonas aeruginosa and Klebsiella pneumoniae to catheter and cannula pieces, respectively. We found that the GlmU inhibitor was mainly effective in preventing the adherence and biofilm formation by gram-positive organisms. The combination of NEM and protamine sulfate may, therefore, be tried as anti-infective coatings for medical devices such as catheters and cannulas, and thus help in overcoming microbial resistance in the current era of increasing device-associated hospital infections.en_US
dc.identifier.citationSuman Ethel, D'souza Sushma Janet, Jacob Pyari, Sushruth M R, Kotian M Shashidhar. Anti-biofilm and anti-adherence activity of Glm-U inhibitors. Indian Journal of Medical Sciences. 2011 Sept; 65(9) 387-392.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/145695
dc.language.isoenen_US
dc.source.urihttps://www.indianjmedsci.org/article.asp?issn=0019-5359;year=2011;volume=65;issue=9;spage=387;epage=392;aulast=Sumanen_US
dc.subjectAnti-biofilmen_US
dc.subjectmedical devicesen_US
dc.subjectN-Acetylglucosamine-1-phosphate uridyltransferaseen_US
dc.subjectN-ethyl maleimideen_US
dc.subjectprotamine sulfateen_US
dc.subject.meshBacterial Adhesion --drug effects
dc.subject.meshBiofilms --drug effects
dc.subject.meshCatheters --adverse effects
dc.subject.meshCatheters --microbiology
dc.subject.meshCross Infection --microbiology
dc.subject.meshCross Infection --prevention & control
dc.subject.meshEthylmaleimide --analogs & derivatives
dc.subject.meshMultienzyme Complexes
dc.subject.meshN-Ethylmaleimide-Sensitive Proteins
dc.subject.meshNucleotidyltransferases
dc.subject.meshProtamines
dc.subject.meshSurface Properties
dc.titleAnti-biofilm and anti-adherence activity of Glm-U inhibitors.en_US
dc.typeArticleen_US
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