2-ethylpyridine, a cigarette smoke component, causes mitochondrial damage in human retinal pigment epithelial cells in vitro.
| dc.contributor.author | Mansoor, S | |
| dc.contributor.author | Gupta, N | |
| dc.contributor.author | Falatoonzadeh, P | |
| dc.contributor.author | Kuppermann, B D | |
| dc.contributor.author | Kenney, M C | |
| dc.date.accessioned | 2015-01-27T04:40:52Z | |
| dc.date.available | 2015-01-27T04:40:52Z | |
| dc.date.issued | 2014-01 | |
| dc.description.abstract | Purpose: Our goal was to identify the cellular and molecular effects of 2-ethylpyridine (2-EP, a component of cigarette smoke) on human retinal pigment epithelial cells (ARPE-19) in vitro. Materials and Methods: ARPE-19 cells were exposed to varying concentrations of 2-EP. Cell viability (CV) was measured by a trypan blue dye exclusion assay. Caspase-3/7 and caspase-9 activities were measured by fl uorochrome assays. The production of reactive oxygen/nitrogen species (ROS/RNS) was detected with a 2’,7’-dichlorodihydrofl uorescein diacetate dye assay. The JC-1 assay was used to measure mitochondrial membrane potential (m). Mitochondrial redox potential was measured using a RedoxSensor Red kit and mitochondria were evaluated with Mitotracker dye. Results: Aft er 2-EP exposure, ARPE-19 cells showed signifi cantly decreased CV, increased caspase-3/7 and caspase-9 activities, elevated ROS/RNS levels, decreased m value and decreased redox fl uorescence when compared with control samples. Conclusions: These results show that 2-EP treatment induced cell death by caspase-dependent apoptosis associated with an oxidative stress and mitochondrial dysfunction. These data represent a possible mechanism by which smoking contributes to age-related macular degeneration and other retinal diseases and identify mitochondria as a target for future therapeutic interventions. | en_US |
| dc.identifier.citation | Mansoor S, Gupta N, Falatoonzadeh P, Kuppermann B D, Kenney M C. 2-ethylpyridine, a cigarette smoke component, causes mitochondrial damage in human retinal pigment epithelial cells in vitro. Indian Journal of Ophthalmology. 2014 Jan ; 62 (1): 16-22. | en_US |
| dc.identifier.uri | https://imsear.searo.who.int/handle/123456789/155500 | |
| dc.language.iso | en | en_US |
| dc.source.uri | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3955065/ | en_US |
| dc.subject | 2-ethylpyridine | en_US |
| dc.subject | apoptosis | en_US |
| dc.subject | ARPE-19 cells | en_US |
| dc.subject | cigarett e smoke toxicant | en_US |
| dc.subject | mitochondrial membrane potential | en_US |
| dc.subject | reactive oxygen/nitrogen species | en_US |
| dc.title | 2-ethylpyridine, a cigarette smoke component, causes mitochondrial damage in human retinal pigment epithelial cells in vitro. | en_US |
| dc.type | Article | en_US |